ORP150 (HYOU1) (NM_001130991) Human 3' UTR Clone

CAT#: SC214545

3`UTR clone of hypoxia up-regulated 1 (HYOU1) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: HYOU1
• Synonyms: GRP-170; Grp170; HSP12A; IMD59; ORP-150; ORP150
• ACCN: NM_001130991
• Insert Size: 1407
• Sequence Data:
  Insert Sequence

  >SC214545 3'UTR clone of NM_001130991
  The sequence shown below is from the reference sequence of NM_001130991. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCCTTTGAAGAACGACGAACTATAACCCCCACCTCTGTTTTCCCCATTCATCTCCACCCCCTTCCCCCAC
  CACTTCTATTTATTTAACATCGAGGGTTGGGGGAGGGGTTGGTCCTGCCCTCGGCTGGAGTTCCTTTCTC
  ACCCCTGTGATTTGGAGGTGTGGAGAAGGGGAAGGGAGGGACAGCTCACTGGTTCCTTCTGCAGTACCTC
  TGTGGTTAAAAATGGAAACTGTTCTCCTCCCCAGCCCCACTCCCTGTTCCCTACCCATATAGGCCCTAAA
  TTTGGGAAAAATCACTATTAATTTCTGAATCCTTTGCCTGTGGGTAGGAAGAGAATGGCTGCCAGTGGCT
  GATGGGTCCCGGTGATGGGAAGGGTATCAGGTTGCTGGGGAGTTTCCACTCTTCTCTGGTGATTGTTCCT
  TCCCTCCCTTCCTCTCCCACCATGCGATGAGCATCCTTTCAGGCCAGTGTCTGCAGAGCCTCAGTTACCA
  GGTTTGGTTTCTGAGTGCCTATCTGTGCTCTTTCCTCCCTCTGCGGGCTTCTCTTGCTCTGAGCCTCCCT
  TCCCCATTCCCCATCCAGCTCCTTTCCCCCTGGGTTTCCTTGGCTTCCTGCAGCAAATTGGGCAGTTCTC
  TGCCCCTTGCCTAAAAGCCTGTACCTCTGGATTGGCGGAAGTAAATCTGGAAGGATTCTCACTCGTATTT
  CCCACCCCTAGTGGCCAGAGGAGGGAGGGGCACAGTGAAGAAGGGAGCCCACCACCTCTCCGAAGAGGAA
  AGCCACGTAGAGTGGTTGGCATGGGGTGCCAGCATCGTGCAAGCTCTGTCATAATCTGCATCTTCCCAGC
  AGCCTGGTACCCCAGGTTCCTGTAACTCCCTGCCTCCTCCTCTCTTCTGCTGTTCTGCTCCTCCCAGACA
  GAGCCTTTCCCTCACCCCCTGACCCCCTGGGCTGACCAAAATGTGCTTTCTACTGTGAGTCCCTATCCCA
  AGATCCTGGGGAAAGGAGAGACCATGGTGTGAATGTAGAGATGCCACCTCCCTCTCTCTGAGGCAGGCCT
  GTGGATGAAGGAGGAGGGTCAGGGCTGGCCTTCCTCTGTGCATCACTCTGCTAGGTTGGGGGCCCCCGAC
  CCACCATACCTACGCCTAGGGAGCCCGTCCTCCAGTATTCCGTCTGTAGCAGGAGCTAGGGCTGCTGCCT
  CAGCTCCAAGACAAGAATGAACCTGGCTGTGTCAGTCATTTTGTCTTTTCCTTTTTTTTTTTTTTTTGCC
  ACATTGGCAGAGATGGGACCTAAGGGTCCCACCCCTCACCCCACCCCCACCTCTTCTGTATGTTTGAATT
  CTTTCAGTAGCTGTTGATGCTGGTTGGACAGGTTTGAGTCAAATTGTACTTTGCTCCATTGTTAATTGAG
  AAACTGT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_001130991.1
• Summary: The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
• Locus ID: 10525