DLST (NM_001933) Human 3' UTR Clone

CAT#: SC214562

3`UTR clone of dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex) (DLST) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: DLST
• Synonyms: DLTS
• ACCN: NM_001933
• Insert Size: 1464 bp
• Sequence Data:
  Insert Sequence

  >SC214562 3'UTR clone of NM_001933
  The sequence shown below is from the reference sequence of NM_001933. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TCAAGGCAGCGGTAGAGGATCCCAGAGTCCTCCTCCTGGATCTTTAGGAGGAACCCACACACCCTACAAG
  TTGATCATGCAGGAACTGAAAACCAGTCTTCTCCCTGTCCCCTCATGGGTCCCGGGTTAGCCTGGTGACA
  GGCAGACACATGCTGTTGGCCTCAAGCAAGGAAGCAGAGCACTGTGTAACCAGCAGTCACAGGTCTTTTC
  TTGGCGTTCCTGCCAGGCTCTCCCTCTCTGCACCTGTCTCATAGCCTCGAATATCTTAATTCCTTAGGCT
  TAAGAGAGAGAGCCTTAATGGATGCTCATTCATATTCCTGCCTTTCTTCCATCAGCTCTCTGCAAAGATG
  ATTTTGCTTTTCCCTAGTGCTGGTATACTATAGAGAAACCCCTGGGGACCATGTGATTAAGTTCCTATCT
  TTTGAAAGTTTGTTCTGCAGAGACTTCTAGGAGGATGCTGTGCCTCCCAAGCTCAGAGCAGCCTCTGTCC
  TGGCTGTGCACATTCTCCCTTGATTCCACTTGTGTGGAGGGATTGAACACAGGCAAAGAGGTGCTGCTTT
  GCTTCTTCAATGGCACCTTCATTCTCCGTTGTCATTGACTTCAAGATGCCTCTTCTACCTCTTCCAGGAA
  GCACAGGCCAGGGGATCTGGGTGTGTGAGTGGGAGGAGAGGGCAGAGGTCCCCTGAGGTCATGCATTGTA
  ATCATCATAGAAGGAGAGCCCAGGCCTGCCCTCACGCTCTCCATCATAGGCTGACACCAAGAAGACTCGT
  CTTGGCACAATCTCACACAGCTGGGGCTGTAGCAACCCTTTCCAACCCCTTTGCTGGTTGCTGGGCCTCA
  TTCTAGCACCTTGTTCTTAGAGCAGATTCTAGCACATCATGGCAGTGGGACCAAGCGTGGTCCCGAGGAA
  GGGCCAGAGCCTGGTAGAGACTAGGGAAGGGAGGTCTCCTCTAGACTGACTCACATTGCCTTGAGCTTTT
  CAGTTAAGTTGCTGTAAGCACCTGGGCTGAGGAGGCAGTTTTTGTTCCTTCCTGCGTTATAGCGGGGCCT
  TGTCTCTTCCTCTGCAGGACACAGATCTGGAGGACGTGGACTGGGGTAGGAAACCACCCTGAGGGTGTTA
  GTACCTAGTGGTGAAACGGATGAGGTCATTTCTAAGGTGTGTTGCCCGTGGATCTGGGCACAATCATTGG
  AATTCCTTGGAGCCACTGGGATTCATGGCTTTGTATCCAACTGCATCCAGGCCTGAGGCTGCTGACGTTT
  GACACCAGGGCCAGTAGAGAGTGCCCTTTTGTATCTTAAGCCAAGAAGTGAGGCCTGGGGGTGGGGGAGG
  GGGGAAGGGGTGGGAGCCAATACTGAGTGCCTGCAGCATCTACTACTCTGTCTTCACTATTCAGAACTTG
  TAACTAAAGTATTTAAAGAAACTGATTTTAAATGCAAATTAAAGGGCAGATATTCTCAAACAGG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001933.4
• Summary: 'This gene encodes a mitochondrial protein that belongs to the 2-oxoacid dehydrogenase family. This protein is one of the three components (the E2 component) of the 2-oxoglutarate dehydrogenase complex that catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]'
• Locus ID: 1743