PRKACA (NM_207518) Human 3' UTR Clone

CAT#: SC214564

3`UTR clone of protein kinase cAMP-dependent catalytic alpha (PRKACA) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PRKACA
• Synonyms: PKACA; PPNAD4
• ACCN: NM_207518
• Insert Size: 1441 bp
• Sequence Data:
  Insert Sequence

  >SC214564 3'UTR clone of NM_207518
  The sequence shown below is from the reference sequence of NM_207518. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GAGAAGTGTGGCAAGGAGTTTTCTGAGTTTTAGGGGCATGCCTGTGCCCCCATGGGTTTTCTTTTTTCTT
  TTTTCTTTTTTTTGGTCGGGGGGGTGGGAGGGTTGGATTGAACAGCCAGAGGGCCCCAGAGTTCCTTGCA
  TCTAATTTCACCCCCACCCCACCCTCCAGGGTTAGGGGGAGCAGGAAGCCCAGATAATCAGAGGGACAGA
  AACACCAGCTGCTCCCCCTCATCCCCTTCACCCTCCTGCCCCCTCTCCCACTTTTCCCTTCCTCTTTCCC
  CACAGCCCCCCAGCCCCTCAGCCCTCCCAGCCCACTTCTGCCTGTTTTAAACGAGTTTCTCAACTCCAGT
  CAGACCAGGTCTTGCTGGTGTATCCAGGGACAGGGTATGGAAAGAGGGGCTCACGCTTAACTCCAGCCCC
  CACCCACACCCCCATCCCACCCAACCACAGGCCCCACTTGCTAAGGGCAAATGAACGAAGCGCCAACCTT
  CCTTTCGGAGTAATCCTGCCTGGGAAGGAGAGATTTTTAGTGACATGTTCAGTGGGTTGCTTGCTAGAAT
  TTTTTTAAAAAAACAACAATTTAAAATCTTATTTAAGTTCCACCAGTGCCTCCCTCCCTCCTTCCTCTAC
  TCCCACCCCTCCCATGTCCCCCCATTCCTCAAATCCATTTTAAAGAGAAGCAGACTGACTTTGGAAAGGG
  AGGCGCTGGGGTTTGAACCTCCCCGCTGCTAATCTCCCCTGGGCCCCTCCCCGGGGAATCCTCTCTGCCA
  ATCCTGCGAGGGTCTAGGCCCCTTTAGGAAGCCTCCGCTCTCTTTTTCCCCAACAGACCTGTCTTCACCC
  TTGGGCTTTGAAAGCCAGACAAAGCAGCTGCCCCTCTCCCTGCCAAAGAGGAGTCATCCCCCAAAAAGAC
  AGAGGGGGAGCCCCAAGCCCAAGTCTTTCCTCCCAGCAGCGTTTCCCCCCAACTCCTTAATTTTATTCTC
  CGCTAGATTTTAACGTCCAGCCTTCCCTCAGCTGAGTGGGGAGGGCATCCCTGCAAAAGGGAACAGAAGA
  GGCCAAGTCCCCCCAAGCCACGGCCCGGGGTTCAAGGCTAGAGCTGCTGGGGAGGGGCTGCCTGTTTTAC
  TCACCCACCAGCTTCCGCCTCCCCCATCCTGGGCGCCCCTCCTCCAGCTTAGCTGTCAGCTGTCCATCAC
  CTCTCCCCCACTTTCTCATTTGTGCTTTTTTCTCTCGTAATAGAAAAGTGGGGAGCCGCTGGGGAGCCAC
  CCCATTCATCCCCGTATTTCCCCCTCTCATAACTTCTCCCCATCCCAGGAGGAGTTCTCAGGCCTGGGGT
  GGGGCCCCGGGTGGGTGCGGGGGCGATTCAACCTGTGTGCTGCGAAGGACGAGACTTCCTCTTGAACAGT
  GTGCTGTTGTAAACATATTTGAAAACTATTACCAATAAAGT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_207518.1
• Summary: 'This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. [provided by RefSeq, Jan 2015]'
• Locus ID: 5566