Stromal interaction molecule 1 (STIM1) (NM_003156) Human 3' UTR Clone

CAT#: SC214586

3`UTR clone of stromal interaction molecule 1 (STIM1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: STIM1
• Synonyms: D11S4896E; GOK; IMD10; STRMK; TAM; TAM1
• ACCN: NM_003156
• Insert Size: 1434 bp
• Sequence Data:
  Insert Sequence

  >SC214586 3'UTR clone of NM_003156
  The sequence shown below is from the reference sequence of NM_003156. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCTCAAAATCTTTAAGAAGCCTCTTAAGAAGTAGGCAGGATGGGGTGGCAGTAAAGGGACAGCTTGTCCT
  TCCCTGGGTGTTCTGTCTCTCCTTCCCTCCCTTCCTTCAAGATAACTGGCCCCAAGAGTGGGGCATGGGA
  AGGGCTGGTCCAGGGGTCTGGGCACTGTACATACCTGCCCCCTCATCCTTGGGTCCTTCATTATTATTTA
  TTAACTGACCACCATGGCCTGCCTGCCCTGCCTCCGTCCCAACCATGGGCTGCTGCTGTCACTCCCTCTC
  CACTTCAGTGCATGTCTTAGTTGCTGTTCCCTCAGCTCCCAGCTCCACCTCTGGGGTTCAGCTTCTGTCT
  CTGCTGTCCCAGTTTTGAGGTTTGGTTTCTTGTTTCTGTCTCTTGCTTTCGGGCTCCTCCCTCCCACCAC
  TCCCCAACTTCCCCTAGCAGTTGCAGGGAAGATAGGACGAGTAGCTTCTGACATGTGTGCCTCAGATCTG
  TTCCACCCCACTCACAGTGGTTCTGTTTGCTCCAGACTGGGGCTAGGGCCTAATCTTTGAAGTTTGTTCT
  TTGGTATTGATGTGGGTCAGAAGGAGCCTCATCCTAATCTCACTCAGGCCTCCAGGGATCCATGGGGGAG
  TGAAACCAATTCTCAGAGAACAACCCACCAGAGACTTTTAAAGAGAGGCCAGGCTTGGGAATGGGTTGGG
  AGAGGCATCTGTTCATTGGAGCATGAGTGGATGCCAGAACTGTAGGTTATAAGGCAGTCACTTTTTCTCT
  CTACTCCCACCCACACCTGCCTCCCTCTTACCCCTGCTCCCCCACACTGCAGGAGGATTTGTCTCTAAGA
  GGTGCTGCCCCAAAGCTCCCCAAGCATCAATACTCCTAGGGCTCAGGACAAGTGGCTCCCCTGGCCAGGA
  GAGCCACAGCCATGATACAGGGCTCTTATGGAGCCCTGGAGTTGTTGGGCAAGGATGCTGTCATTTTTTG
  AACCAAAAGACAAACAGGTTAAAAGGAAAAAAAGTAATCTGAATTTCCCAAGTGCCTACGCTGCATATTC
  CCCTTGTTAGATCCCATTTTCATGTTACTTTGTAGCCTTGGCCAGAGGCTCAAAAAGGACACAACCAGTT
  TGGGGAAGGGGTGGCTAAGGAAGATGGTATAGGTGAAGGCGGCTGTGTGACCACTTTCCCCCACCCTTCC
  CACCCTCTAGACAACTCTCTCCCTTACCTGTTTTTGCTATGGCTGTAAAGGTATTTTTCCTCTGCCCCAC
  TCCCTGCCATACCTTTATCCTGGGATCCTATTTTGGGCCTGGGGTGGGTATACCTGGGGCTGGTCTTAGG
  AGGGTGCTAGGCTGCAGACTGCCTTGTACTCCCTGGACACCCTCAAATGGGGTTTTCTGTGTTATTTCAT
  AAAATTCTTTGAAGTCCAATAAAGCATGTAGGAG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003156.3
• Summary: 'This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classic Kaposi sarcoma, immunodeficiency due to defects in store-operated calcium entry (SOCE) in fibroblasts, ectodermal dysplasia and tubular aggregate myopathy. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]'
• Locus ID: 6786