TJP1 (NM_003257) Human 3' UTR Clone

CAT#: SC214630

3`UTR clone of tight junction protein 1 (zona occludens 1) (TJP1) transcript variant 1 for miRNA target validation

Reconstitution Protocol

USD 560.00

2 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Symbol TJP1
Synonyms ZO-1
ACCN NM_003257
Insert Size 1477 bp
Sequence Data
>SC214630 3'UTR clone of NM_003257
The sequence shown below is from the reference sequence of NM_003257. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon


CAATTGGCAGAGCTCAGAATTCAAGCGATCGC

TCTCGTTGGAGCAAACTGTGTTTCTGTCCTTATTGACCACTTTTAACTCTTGAAATATAGGAACTTAAAT
AATGTGAAACTGGATTAAACTTAATCTAAATGGAACCACTCTATCAAGTATTATACCTTTTTTAGAGTTG
ATACTACAGTTTGTTAGTATGAGGCATTTGTTTGAACTGATAAAGATGAGTGAGCATGCCCCTGAACCAT
GGTCGGAAAACATGCTACACACTGCATGTTTGTGATTGACGGGACTGTTGGTATTGGCTAGAGGTTCAAA
GATATTTTGCTTTGTGATTTTTGTAATTTTTTTATCGTCACTGCTTAACTTCACATATTGATTTCCGTTA
AAATACCAGCCAGTAAATGGGGGTGCATTTGAGGTCTGTTCTTTCCAAAGTACACTGTTTCAAACTTTAC
TATGGCCCTGGCCTAGCATACGTACACATTTTATTTTATTATGCATGAAGTAATATGCACACATTTTTTA
AATGCACCTGGAATATATAACCAGTGTTGTGGATTTAACAGAAATGTACAGCAAGGAGATTTACAACTGG
GGGAGGGTGAAGTGAAGACAATGACTTACTGTACATGAAAACACATTTTTCTTAGGGAAGGATACAAAAG
CATGTGAGACTGGTTCCATGGCCTCTTCAGATCTCTAACTTCACCATATTACCACAGACATACTAACCAG
CAGAAATGCCTTACCCTCATGTTCTTAATTCTTAGCTCATTCTCCTTGTGTTACTAAGTTTTTATGGCTT
TTGTGCATTATCTAGATACTGTATCATGACAAAGACTGAGTACGTTGTGCATTTGGTGGTTTCAGAAATG
TGTTATCACCCAGAAGAAAATAGTGGTGTGATTTGGGGATATTTTTTTCTTTTCTTTTCTTTTCTTTTTT
TTTTTTTTTGACAAGGGGCAGTGGTGGTTTTCTGTTCTTTCTGGCTATGCATTTGAAAATTTTGATGTTT
TAAGGATGCTTGTACATAATGCGTGCATACCACTTTTGTTCTTGGTTTGTAAATTAACTTTTATAAACTT
TACCTTTTTTATACATAAACAAGACCACGTTTCTAAAGGCTACCTTTGTATTCTCTCCTGTACCTCTTGA
GCCTTGAACTTTGACCTCTGCAGCAATAAAGCAGCGTTTCTATGACACATGCAAGGTCATTTTTTTTAAG
AAAAAGGATGCACAGAGTTGTTACATTTTTAAGTGCTGCATTTAAAAGATACAGTTACTCAGAATTCTCT
AGTTTGATTAAATTCTTGCAAAGTATCCCTACTGTAATTTGTGATACAATGCTGTGCCCTAAAGTGTATT
TTTTTACTAATAGACAATTTATTATGGCACATCAGCACGATTTCTGTTTAGATAATACACCACTACATTC
TGTTAATCATTAGGTGTGACTGAATTTCTTTTGCCGTTATTAAAAATCTCAAATTTCTAAATCTCCAAAA
TAAAACT

ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
Restriction Sites SgfI-MluI     
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Reference Data
RefSeq NM_003257.3
Summary 'This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]'
Locus ID 7082

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.