UBE2D3 (NM_181890) Human 3' UTR Clone

CAT#: SC214669

3`UTR clone of ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog yeast) (UBE2D3) transcript variant 6 for miRNA target validation

Reconstitution Protocol

USD 560.00

4 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Symbol UBE2D3
Synonyms E2(17)KB3; UBC4/5; UBCH5C
ACCN NM_181890
Insert Size 1440 bp
Sequence Data
>SC214669 3'UTR clone of NM_181890
The sequence shown below is from the reference sequence of NM_181890. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon


CAATTGGCAGAGCTCAGAATTCAAGCGATCGC

GGACTCAGAAGTATGCCATGTGATGCTACCTTAAAGTCAGAATAACCTGCATTATAGCTGGAATAAACTT
TAAATTACTGTTCCTTTTTTGATTTTCTTATCCGGCTGCTCCCCTATCAGACCTCATCTTTTTTAATTTT
ATTTTTTGTTTACCTCCCTCCATTCATTCACATGCTCATCTGAGAAGACTTAAGTTCTTCCAGCTTTGGA
CAATAACTGCTTTTAGAAACTGTAAAGTAGTTACAAGAGAACAGTTGCCCAAGACTCAGAATTTTTAAAA
AAAAAAATGGAGCATGTGTATTATGTGGCCAATGTCTTCACTCTAACTTGGTTATGAGACTAAAACCATT
CCTCACTGCTCTAACATGCTGAAGAAATCATCTGAGGGGGAGGGAGATGGATGCTCAGTTGTCACATCAA
AGGATACAGCATTATTCTAGCAGCATCCATTCTTGTTTAAGCCTTCCACTGTTAGAGATTTGAGGTTACA
TGATATGCTTTATGCTCATAACTGATGTGGCTGGAGAATTGGTATTGAATTTATAGCATCAGCAGAACAG
AAAATGTGATGTATTTTATGCATGTCAATAAAGGAATGACCTGTTCTTGTTCTACAGAGAATGGAAATTG
GAAGTCAAACACCCTTTGTATTCCAAAATAGGGTCTCAAACATTTTGTAATTTTCATTTAAATTGTTAGG
AGGCTTGGAGCTATTAGTTAATCTATCTTCCAATACACTGTTTAATATAGCACTGAATAAATGATGCAAG
TTGTCAATGGATGAGTGATCAACTAATAGCTCTGCTAGTAATTGATTTATTTTTCTTCAATAAAGTTGCA
TAAACCAATGAGTTAGCTGCCTGGATTAATCAGTATGGGAAACAATCTTTTGTAAATGCAAAGCTGTTTT
TTGTATATACTGTTGGGATTTGCTTCATTGTTTGACATCAAATGATGATGTAAAGTTCGAAAGAGTGAAT
ATTTTGCCATGTTCAGTTAAAGTGCACAGTCTGTTACAGGTTGACACATTGCTTGACCTGATTTATGCAG
AATTAATAAGCTATTTGGATAGTGTAGCTTTAATGTGCTGCACATGATACTGGCAGCCCTAGAGTTCATA
GATGGACTTTTGGGACCCAGCAGTTTTGAAATGTGTTTATGGAGTTTAAGAAATTTATTTTCCAGGTGCA
GCCCCTGTCTAACTGAAATTTCTCTTCACCTTGTACACTTGACAGCTGAAAAAAAACAACATGGGAGTAA
TAATGGGTCAAAATTTGCAAAATAAAGTACTGTTTTGGTGTGGGAGTTGTCATGAGGCTGTGTTGAAGTG
ACTTATCTATGTGGGATATTGAGTATCCATTGAAATGGATTTGTTCAGCCATTTACATTAATGAGCATTT
AAATGCAACAGATATCATTTCAGGTGACTTAACATGAATG

ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
Restriction Sites SgfI-MluI     
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Reference Data
RefSeq NM_181890.1
Summary 'The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. [provided by RefSeq, Jan 2017]'
Locus ID 7323

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.