UBE2D3 (NM_003340) Human 3' UTR Clone

CAT#: SC214672

3`UTR clone of ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog yeast) (UBE2D3) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: UBE2D3
• Synonyms: E2(17)KB3; UBC4/5; UBCH5C
• ACCN: NM_003340
• Insert Size: 1440 bp
• Sequence Data:
  Insert Sequence

  >SC214672 3'UTR clone of NM_003340
  The sequence shown below is from the reference sequence of NM_003340. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGACTCAGAAGTATGCCATGTGATGCTACCTTAAAGTCAGAATAACCTGCATTATAGCTGGAATAAACTT
  TAAATTACTGTTCCTTTTTTGATTTTCTTATCCGGCTGCTCCCCTATCAGACCTCATCTTTTTTAATTTT
  ATTTTTTGTTTACCTCCCTCCATTCATTCACATGCTCATCTGAGAAGACTTAAGTTCTTCCAGCTTTGGA
  CAATAACTGCTTTTAGAAACTGTAAAGTAGTTACAAGAGAACAGTTGCCCAAGACTCAGAATTTTTAAAA
  AAAAAAATGGAGCATGTGTATTATGTGGCCAATGTCTTCACTCTAACTTGGTTATGAGACTAAAACCATT
  CCTCACTGCTCTAACATGCTGAAGAAATCATCTGAGGGGGAGGGAGATGGATGCTCAGTTGTCACATCAA
  AGGATACAGCATTATTCTAGCAGCATCCATTCTTGTTTAAGCCTTCCACTGTTAGAGATTTGAGGTTACA
  TGATATGCTTTATGCTCATAACTGATGTGGCTGGAGAATTGGTATTGAATTTATAGCATCAGCAGAACAG
  AAAATGTGATGTATTTTATGCATGTCAATAAAGGAATGACCTGTTCTTGTTCTACAGAGAATGGAAATTG
  GAAGTCAAACACCCTTTGTATTCCAAAATAGGGTCTCAAACATTTTGTAATTTTCATTTAAATTGTTAGG
  AGGCTTGGAGCTATTAGTTAATCTATCTTCCAATACACTGTTTAATATAGCACTGAATAAATGATGCAAG
  TTGTCAATGGATGAGTGATCAACTAATAGCTCTGCTAGTAATTGATTTATTTTTCTTCAATAAAGTTGCA
  TAAACCAATGAGTTAGCTGCCTGGATTAATCAGTATGGGAAACAATCTTTTGTAAATGCAAAGCTGTTTT
  TTGTATATACTGTTGGGATTTGCTTCATTGTTTGACATCAAATGATGATGTAAAGTTCGAAAGAGTGAAT
  ATTTTGCCATGTTCAGTTAAAGTGCACAGTCTGTTACAGGTTGACACATTGCTTGACCTGATTTATGCAG
  AATTAATAAGCTATTTGGATAGTGTAGCTTTAATGTGCTGCACATGATACTGGCAGCCCTAGAGTTCATA
  GATGGACTTTTGGGACCCAGCAGTTTTGAAATGTGTTTATGGAGTTTAAGAAATTTATTTTCCAGGTGCA
  GCCCCTGTCTAACTGAAATTTCTCTTCACCTTGTACACTTGACAGCTGAAAAAAAACAACATGGGAGTAA
  TAATGGGTCAAAATTTGCAAAATAAAGTACTGTTTTGGTGTGGGAGTTGTCATGAGGCTGTGTTGAAGTG
  ACTTATCTATGTGGGATATTGAGTATCCATTGAAATGGATTTGTTCAGCCATTTACATTAATGAGCATTT
  AAATGCAACAGATATCATTTCAGGTGACTTAACATGAATG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003340.5
• Summary: 'The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. [provided by RefSeq, Jan 2017]'
• Locus ID: 7323