IKK beta (IKBKB) (NM_001556) Human 3' UTR Clone

CAT#: SC214745

3`UTR clone of inhibitor of kappa light polypeptide gene enhancer in B-cells kinase beta (IKBKB) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: IKBKB
• Synonyms: IKK-beta; IKK2; IKKB; IMD15; IMD15A; IMD15B; NFKBIKB
• ACCN: NM_001556
• Insert Size: 1482 bp
• Sequence Data:
  Insert Sequence

  >SC214745 3'UTR clone of NM_001556
  The sequence shown below is from the reference sequence of NM_001556. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGAAGAGCACAGCTGCCTGGAGCAGGCCTCATGATGTGGGGGGACTCGACCCCCTGACATGGGGCAGCCC
  ATAGCAGGCCTTGTGCAGTGGGGGGACTCGACCCCCTGACATGGGGCTGCCTGGAGCAGGCCGCGTGACG
  TGGGGCTGCCTGGCCGCGGCTCTCACATGGTGGTTCCTGCTGCACTGATGGCCCAGGGGTCTCTGGTATC
  CAGATGGAGCTCTCGCTTCCTCAGCAGCTGTGACTTTCACCCAGGACCCAGGACGCAGCCCTCCGTGGGC
  ACTGCCGGCGCCTTGTCTGCACACTGGAGGTCCTCCATTACAGAGGCCCAGCGCACATCGCTGGCCCCAC
  AAACGTTCAGGGGTACAGCCATGGCAGCTCCTTCCTCTGCCGTGAGAAAAGTGCTTGGAGTACGGTTTGC
  CACACACGTGACTGGACAGTGTCCAATTCAAATCTTTCAGGGCAGAGTCCGAGCAGCGCTTGGTGACAGC
  CTGTCCTCTCCTGCTCTCCAAAGGCCCTGCTCCCTGTCCTCTCTCACTTTACAGCTTGTGTTTCTTCTGG
  ATTCAGCTTCTCCTAAACAGACAGTTTAATTATAGTTGCGGCCTGGCCCCATCCTCACTTCCTCTTTTTA
  TTTCACTGCTGCTAAAATTGTGTTTTTACCTACTACTTTGGTGGTTGTCCTCTTTTCGGCAAAGTTGGAG
  CGAGTGCCAAGCTCTCCATCTGTGGTCCTTTCTGCCAAGAGCGACTCATAGTAACCAGGATGGGAGAGCA
  GCTGCCTTATTCTGAATCCCAAAAATTACTTGGGGGTGATTGTCACAGAGGAGGGACAGAAAGGGTATCT
  GCTGACCACCAGCCTGCCTACCCATGCCCATGTCTCCATTCCTGCTCAAGCGTGTGTGCTGGGCCGGGGA
  GTCCCTGTCTCTCACAGCATCTAGCAGTATTATTAAATGGATTCATTTTAAAAATAGCTCCTATATTTTG
  TAACATGTCTCAAACACTCATACTGGGTTCCACAATCCACTGTTAGAATACCTATGGTTAGGGCTTCTGA
  ACTAAAATAATGGAAAATTTTAACAATTTGTATAGTGCCTGGATCATTACTAGTGCCATAACCCTGCTTC
  TTCAACATTTCACAGAACTTCTCTTTTATATAAAGGCAAGAGCACAAAATGAGTTCAGATGATCACAAAC
  AGGTGAGTTTTGTTGGAGAAGAAAGTTGGAGTAGGAGACTTTCACAAGTGGTTTCCATGGAGATAGAATG
  AAGCATTCTGTGGTCAAGTAAGTTTAGGGAGCTATTCATGTTTCACTTGCTTTGTGGAGATTCACACTAT
  GCACTGGGAAAGTATCTGAAAAGTCTTATAATAAAGAAACAGGCTTAACTTTGTGTAAGAACACTGTTTA
  TCAATGTCATTTGGCTATAGAAACATTTTCTCCTGCTGATTGTGTGTGTGAAACATGTATTAACATTCCA
  ATGAACTAGCAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001556.1
• Summary: 'The protein encoded by this gene phosphorylates the inhibitor in the inhibitor/NF-kappa-B complex, causing dissociation of the inhibitor and activation of NF-kappa-B. The encoded protein itself is found in a complex of proteins. Several transcript variants, some protein-coding and some not, have been found for this gene. [provided by RefSeq, Sep 2011]'
• Locus ID: 3551