CYP51A1 (NM_001146152) Human 3' UTR Clone

CAT#: SC215038

3`UTR clone of cytochrome P450 family 51 subfamily A polypeptide 1 (CYP51A1) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CYP51A1
• Synonyms: CP51; CYP51; CYPL1; LDM; P450-14DM; P450L1
• ACCN: NM_001146152
• Insert Size: 1531 bp
• Sequence Data:
  Insert Sequence

  >SC215038 3'UTR clone of NM_001146152
  The sequence shown below is from the reference sequence of NM_001146152. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCCTGAAAACCCAGTTATCCGTTACAAACGAAGATCAAAATGAAAAAGGTTGCAAGGAACGAATATATGT
  GATTATCACTGTAAGCCACAAAGGCATTCGAAGAGAATGAAGTGTACAAAACAACTCTTGTAGTTTACTG
  TTTTTTTAAGTGTGTAATTCTAAAAGCCAGTTTATGATTTAGGATTTTGTTAACTGAATGGTTCTATCAA
  ATATAATAGCATTTGAAACATTTTCTAATAGTTATGATACTTATACATGTGCTTTCAGGAAGTTCCTTGG
  TGAAACAATTGTTGAGGGGGGATCTAGGTAATTGGCAGATTCTAAATAATATAATTTCCAGATAGTAATT
  TTAAGAGTACTCATCGCTCTTGCCAAATAAGTTCAGGGTATTCAAATCTTGGACTAGTCCTGCAAGGTAT
  AAAGAATAAAAATCCCAGTGAGATACTTGGAAACCACAGTTTATTATTATTTATCTGGGCAATTATTGTG
  TGTGTGAGGATGGAAGGGTAGGGAATAATCGAACATCTAAAGCCTTGAATAAGAGAATACTAATTGTTTT
  GGTATGATGATACTCAGAAATGGAGATATTATAGGAAAAAGAAATCCTTTGGAATTTTAACTAAAATCAC
  TGCATATGGGAAATTAAGAGATCCAGGACCATATTTGATAAGAGTTCCTAAAAATAATGTAATTATTAAT
  GCTAAAGACTGCTCATGTATCTTGATCTAATTACTAAATAATTACATATTTATTTACCTGATAAATATGT
  ATCTAGTTCTACAAGGTCACATTTATGTGGAAGTCCAAAGTCAAGTCCTTAGGGGATAATTTTGTTTTGG
  CTCAGTTGTTCCCTGCTTCCTTTTTTTTTTTTTTTTTTTGAGATGGAGTCTCGCTCTGTTGCCCAGGCTG
  GAGTGCAGTGGTGCGATCTCAGCTCACTGCATCCTCTGCCTCCCGGGTTCAAGCAATTCTCTGCCTCAGC
  CTCCCAAGTAGTTGGGATTACAGGCACCTGCCACCATGCCTGGCTAATTTTTTGTATTTTTAGTAGAGAC
  GGGGGTTTCACTATGTTGGCTAGGCTGGTCTTGAACTCCTGACCTCGTGATCCACCCGCCTTGGCCTCCC
  AAAGTGCTGGGATTACAGGCATGAGCCACCGCACCTGGCCTTCCCTGCTTCCTCTCTAGAATCCAATTAG
  GGATGTTTGTTACTACTCATATTGATTAAAACAGTTAACAAACTTTTTTCTTTTTAAAATGTGAGATCAG
  TGAACTCTGGTTTTAAGATAATCTGAAACAAGGTCCTTGGGAGTAATAAAATTGGTCACATTCTGTAAAG
  CACATTCTGTTTAGGAATCAACTTATCTCAAATTGTAACTCGGGGCCTAACTATATGAGATGGCTGAAAA
  AATACCACATCGTCTGTTTTCACTAGGTGATGCCAAAATATTTTGCTTTATGTATATTACAGTTCTTTTT
  AAAACACTGGAAGACTCATGTTAAACTCTAATTGTGAAGGCAGAATCTCTGCTAATTTTTC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001146152.1
• Summary: 'This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. Homologous genes are found in all three eukaryotic phyla, fungi, plants, and animals, suggesting that this is one of the oldest cytochrome P450 genes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]'
• Locus ID: 1595