SHC (SHC1) (NM_001130040) Human 3' UTR Clone

CAT#: SC215055

3`UTR clone of SHC (Src homology 2 domain containing) transforming protein 1 (SHC1) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: SHC1
• Synonyms: SHC; SHCA
• ACCN: NM_001130040
• Insert Size: 1530 bp
• Sequence Data:
  Insert Sequence

  >SC215055 3'UTR clone of NM_001130040
  The sequence shown below is from the reference sequence of NM_001130040. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTGTGTCTACAGCAACCTGTGGAGCGGAAACTGTGATCTGCCCTAGCGCTCTCTTCCAGAAGATGCCCTC
  CAATCCTTTCCACCCTATTCCCTAACTCTCGGGACCTCGTTTGGGAGTGTTCTGTGGGCTTGGCCTTGTG
  TCAGAGCTGGGAGTAGCATGGACTCTGGGTTTCATATCCAGCTGAGTGAGAGGGTTTGAGTCAAAAGCCT
  GGGTGAGAATCCTGCCTCTCCCCAAACATTAATCACCAAAGTATTAATGTACAGAGTGGCCCCTCACCTG
  GGCCTTTCCTGTGCCAACCTGATGCCCCTTCCCCAAGAAGGTGAGTGCTTGTCATGGAAAATGTCCTGTG
  GTGACAGGCCCAGTGGAACAGTCACCCTTCTGGGCAAGGGGGAACAAATCACACCTCTGGGCTTCAGGGT
  ATCCCAGACCCCTCTCAACACCCGCCCCCCCCATGTTTAAACTTTGTGCCTTTGACCATCTCTTAGGTCT
  AATGATATTTTATGCAAACAGTTCTTGGACCCCTGAATTCAATGACAGGGATGCCAACACCTTCTTGGCT
  TCTGGGACCTGTGTTCTTGCTGAGCACCCTCTCCGGTTTGGGTTGGGATAACAGAGGCAGGAGTGGCAGC
  TGTCCCCTCTCCCTGGGGATATGCAACCCTTAGAGATTGCCCCAGAGCCCCACTCCCGGCCAGGCGGGAG
  ATGGACCCCTCCCTTGCTCAGTGCCTCCTGGCCGGGGCCCCTCACCCCAAGGGGTCTGTATATACATTTC
  ATAAGGCCTGCCCTCCCATGTTGCATGCCTATGTACTCTACGCCAAAGTGCAGCCCTTCCTCCTGAAGCC
  TCTGCCCTGCCTCCCTTTCTGGGAGGGCGGGGTGGGGGTGACTGAATTTGGGCCTCTTGTACAGTTAACT
  CTCCCAGGTGGATTTTGTGGAGGTGAGAAAAGGGGCATTGAGACTATAAAGCAGTAGACAATCCCCACAT
  ACCATCTGTAGAGTTGGAACTGCATTCTTTTAAAGTTTTATATGCATATATTTTAGGGCTGTAGACTTAC
  TTTCCTATTTTCTTTTCCATTGCTTATTCTTGAGCACAAAATGATAATCAATTATTACATTTATACATCA
  CCTTTTTGACTTTTCCAAGCCCTTTTACAGCTCTTGGCATTTTCCTCGCCTAGGCCTGTGAGGTAACTGG
  GATCGCACCTTTTATACCAGAGACCTGAGGCAGATGAAATTTATTTCCATCTAGGACTAGAAAAACTTGG
  GTCTCTTACCGCGAGACTGAGAGGCAGAAGTCAGCCCGAATGCCTGTCAGTTTCATGGAGGGGAAACGCA
  AAACCTGCAGTTCCTGAGTACCTTCTACAGGCCCGGCCCAGCCTAGGCCCGGGGTGGCCACACCACAGCA
  AGCCGGCCCCCCCTCTTTTGGCCTTGTGGATAAGGGAGAGTTGACCGTTTTCATCCTGGCCTCCTTTTGC
  TGTTTGGATGTTTCCACGGGTCTCACTTATACCAAAGGGAAAACTCTTCATTAAAGTCCG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001130040.1
• Summary: 'This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]'
• Locus ID: 6464