E74 like factor 1 (ELF1) (NM_001145353) Human 3' UTR Clone

CAT#: SC215104

3`UTR clone of E74-like factor 1 (ets domain transcription factor) (ELF1) transcript variant 2 for miRNA target validation

Reconstitution Protocol

USD 670.00

4 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Symbol ELF1
Synonyms EFTUD1; RIA1
ACCN NM_001145353
Insert Size 1504 bp
Sequence Data
>SC215104 3'UTR clone of NM_001145353
The sequence shown below is from the reference sequence of NM_001145353. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon


CAATTGGCAGAGCTCAGAATTCAAGCGATCGC

ACTGCTGGAACCCAACTCTTTTTAGTTAATATACCAAAGCTTATGAATAATTGTTTGTTAATTGAACATT
TTCAATTATATGCAGACTGACTGATTCTAAGATAAATTCTAAGGAGGTTTCTAATTTTGTAATTGTTAAA
AATAGAGTTAATTTTGACTTTGTTAGATGAGGGAGGAAAACTCAACTGTTTCTCTTTGTTATCTAAATGT
TTCAGAATTCAATCGTGAAGGAACAGGCATTTTACACTATGAAGACATTCTTTTGAGATTTTTATTTCAG
TTGCTATATCATAAGCATTTTTAAAGTTTCTTTTCTAATTTTACATTGTATTAGATTTTCTGATTCTTTT
GTAAATACAGAACTTAAATAGAAGGCAACAGGAAATTTATATAGGAACTATTTTCATTCCACTTGTGTAA
GTTAAGTCTTGACTCTTTCAAATGCAAAAAACCTATTTTATGCTTTGTTAAAATTATGGTGTCACTTAGA
TTGACTTTAGTTGACTGCACTATATAATATAGAACTATGAATATGTAGAATAACATGAAAAATTGGAGGT
GCTGGTGGTATGGCTGACCCTGTTTCAGAAGCAGGATAGTATAAAAGCATCAGCCTAAGAATGGCACTCC
CACTAACTAGCTATGTAATCTTGACCTCTTTGGGCTTTAGTTCCTCTCATAAAAGGAAGAGATGTATTGG
ATTAGACTAGATGATCACCACTTTCTCTTCTAGTTCTAATTTTTTTAATTCTAATACCTATATTTTCAAG
TTATGTCAATTAAATCATTATCAGGTTATTTCCTAATGTAAGAATAGCTAAAATGTTGCAGAGAAATAAG
TGACCCAACAAAATTTATTCATCTGTTATGGGTAAGATCTGCCATAAATTCTTCCTAAATAATTTGTTTA
CTAACTCTTTAGGCCACTGTGCTTTGCGGTCCATTAGTAAACTTGTGTTGCTAAGTGCTAAACAGAATAC
TGCTATTTTGAGAGAGTCAAGACTCTTTCTTAAGGGCCAAGAAAGCAACTTGAGCCTTGGGCTAATCTGG
CTGAGTAGTCAGTTATAAAAGCATAATTGCTTTATATTTTGGATCATTTTTTACTGGGGGCGGACTTGGG
GGGGGTTGCATACAAAGATAACATATATATCCAACTTTCTGAAATGAAATGTTTTTAGATTACTTTTTCA
ACTGTAAATAATGTACATTTAATGTCACAAGAAAAAAATGTCTTCTGCAAATTTTCTAGTATAACAGAAA
TTTTTGTAGATGAAAAAAATCATTATGTTTAGAGGTCTAATGCTATGTTTTCATATTACAGAGTGAATTT
GTATTTAAACAAAAATTTAAATTTTGGAATCCTCTAAACATTTTTGTATCTTTAATTGGTTTATTATTAA
ATAAATCATATAAAAATTCTCAGTGTCTGTTTTCAGGCAAAAGTTTCTTAAAGAATAAGTGTGCAGAGAA
TATTACTAGAACATCAGCATTACTTAATGTTTAT

ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
Restriction Sites SgfI-MluI     
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Reference Data
RefSeq NM_001145353.1
Summary 'This gene encodes an E26 transformation-specific related transcription factor. The encoded protein is primarily expressed in lymphoid cells and acts as both an enhancer and a repressor to regulate transcription of various genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2009]'
Locus ID 1997

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.