MADH7 (SMAD7) (NM_005904) Human 3' UTR Clone

CAT#: SC215141

3`UTR clone of SMAD family member 7 (SMAD7) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: SMAD7
• Synonyms: CRCS3; MADH7; MADH8
• ACCN: NM_005904
• Insert Size: 1517 bp
• Sequence Data:
  Insert Sequence

  >SC215141 3'UTR clone of NM_005904
  The sequence shown below is from the reference sequence of NM_005904. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GAGGTCATCTTCAACAGCCGGTAGCCGCGTGCGGAGGGGACAGAGCGTGAGCTGAGCAGGCCACACTTCA
  AACTACTTTGCTGCTAATATTTTCCTCCTGAGTGCTTGCTTTTCATGCAAACTCTTTGGTCGTTTTTTTT
  TTGTTTGTTGGTTGGTTTTCTTCTTCTCGTCCTCGTTTGTGTTCTGTTTTGTTTCGCTCTTTGAGAAATA
  GCTTATGAAAAGAATTGTTGGGGGTTTTTTTGGAAGAAGGGGCAGGTATGATCGGCAGGACACCCTGATA
  GGAAGAGGGGAAGCAGAAATCCAAGCACCACCAAACACAGTGTATGAAGGGGGGCGGTCATCATTTCACT
  TGTCAGGAGTGTGTGTGAGTGTGAGTGTGCGGCTGTGTGTGCACGCGTGTGCAGGAGCGGCAGATGGGGA
  GACAACGTGCTCTTTGTTTTGTGTCTCTTATGGATGTCCCCAGCAGAGAGGTTTGCAGTCCCAAGCGGTG
  TCTCTCCTGCCCCTTGGACACGCTCAGTGGGGCAGAGGCAGTACCTGGGCAAGCTGGCGGCTGGGGTCCC
  AGCAGCTGCCAGGAGCACGGCTCTGTCCCCAGCCTGGGAAAGCCCCTGCCCCTCCTCTCCCTCATCAAGG
  ACACGGGCCTGTCCACAGGCTTCTGAGCAGCGAGCCTGCTAGTGGCCGAACCAGAACCAATTATTTTCAT
  CCTTGTCTTATTCCCTTCCTGCCAGCCCCTGCCATTGTAGCGTCTTTCTTTTTTGGCCATCTGCTCCTGG
  ATCTCCCTGAGATGGGCTTCCCAAGGGCTGCCGGGGCAGCCCCCTCACAGTATTGCTCACCCAGTGCCCT
  CTCCCCTCAGCCTCTCCCCTGCCTGCCCTGGTGACATCAGGTTTTTCCCGGACTTAGAAAACCAGCTCAG
  CACTGCCTGCTCCCATCCTGTGTGTTAAGCTCTGCTATTAGGCCAGCAAGCGGGGATGTCCCTGGGAGGG
  ACATGCTTAGCAGTCCCCTTCCCTCCAAGAAGGATTTGGTCCGTCATAACCCAAGGTACCATCCTAGGCT
  GACACCTAACTCTTCTTTCATTTCTTCTACAACTCATACACTCGTATGATACTTCGACACTGTTCTTAGC
  TCAATGAGCATGTTTAGACTTTAACATAAGCTATTTTTCTAACTACAAAGGTTTAAATGAACAAGAGAAG
  CATTCTCATTGGAAATTTAGCATTGTAGTGCTTTGAGAGAGAAAGGACTCCTGAAAAAAAACCTGAGATT
  TATTAAAGAAAAAAATGTATTTTATGTTATATATAAATATATTATTACTTGTAAATATAAAGACGTTTTA
  TAAGCATCATTATTTATGTATTGTGCAATGTGTATAAACAAGAAAAATAAAGAAAAGATGCACTTTGCTT
  TAATATAAATGCAAATAACAAATGCCAAATTAAAAAAGATAAACACAAGATTGGTGTTTTTTCCTATGGG
  TGTTATCACCTAGCTGAATGTTTTTCTAAAGGAGTTTATGTTCCATT
  
  CGGACCGTTACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-RsrII
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005904.2
• Summary: 'The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. Expression of this gene is induced by TGFBR1. Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]'
• Locus ID: 4092