ME1 (NM_002395) Human 3' UTR Clone

CAT#: SC215142

3`UTR clone of malic enzyme 1 NADP(+)-dependent cytosolic (ME1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ME1
• Synonyms: HUMNDME; MES
• ACCN: NM_002395
• Insert Size: 1547 bp
• Sequence Data:
  Insert Sequence

  >SC215142 3'UTR clone of NM_002395
  The sequence shown below is from the reference sequence of NM_002395. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCCTGAAGAGGTGCAGAAAATACAGACCAAAGTTGACCAGTAGGATAATAGCAAACATTTCTAACTCTAT
  TAATGAGGTCTTTAAACCTTTCATAATTTTTAAAGGTTGGAATCTTTTATAATGATTCATAAGACACTTA
  GATTAAGATTTTACTTTAACAGTCTAAAAATTGATAGAAGAATATCGATATAAATTGGGATAAACATCAC
  ATGAGACAATTTTGCTTCACTTTGCCTTCTGGTTATTTATGGTTTCTGTCTGAATTATTCTGCCTACGTT
  CTCTTTAAAAGCTGTTGTACGTACTACGGAGAAACTCATCATTTTTATACAGGACACTAATGGGAAGACC
  AAAATTACTAATAAATTGACATAACCAACATTAAAACTCATAATTATTTTGTTGACCATTTTGTTAAAAT
  CTACTTTTCAAAAAAAAAAAGCTAGAAATGAATCTAGGCGTAGGTGAACTTTTGCTAAGCAGAAATAACA
  CTACTTTGTTGCCTAGAGAAAGATAACTTCTCAAGTATTTTTATTCCAGTCCTAGATCATATATGTTCTT
  TTGTGCAACGGAATTCTAACAGTTCTAAGAGAAAGATCACTGCTGTTTACAGCGCCTTGTGCAGCCTTAG
  ATTTTAATATTCTTTTGTCATTGTTACATCTCATAGAGTAAAGCTCTTATTACCTTGATCCTGAGTCAGA
  AATCCCACCTGAAATCACCTTTTTTCCCCCTTGATCAAACATCCCATCCTTCAGCTACCATACTGTTGCT
  ACAGGGATTTTGTGGACTGTGGCCCCTGTCCTGAGGTTGGCACCTTCAGTTCAGCACAGCCTGAGCAGTG
  AGAAGGTCTGAAAGGAGAGTATATAGTTAAGATCCTTGAGAAAGGGCTGCCTGAGGAACTGACCTCTTAA
  AGATCTCAGGATCTTTAAGACAACAAGTTAGGTTCCTACTGGAGTTACCTGCCAGAATGGCCTCTTAATT
  AACTCAGGTAATGAAGAGCTAACTGTGTTATAATCATCTTGCTTTTGCCTGAATTTGGAGAAAGTATTAT
  AATTAAGTTCCCAGTATCAGAAATGTCCTTACATAAGATTAAAATATCTTGATGACTAATACCATTCTAT
  GAGAAAGAGTAGTTATATGCCCAGACTGTATTAATTTACTTTAGAAACTAATGTTTGAAGTAATGGAAAA
  AATTTTAAATTATAAAGCTAAGGTGCAATAACATTTGCTACTTATTTATAGAATTATTTGAAGAATTTTG
  TTTTTGAAGTAATGCTTTAAGGAGTATAAGATATTCAAGATAAATTATACTATAAAATGATTTTATTGAA
  AGTTGAAGGTTACACAAATTGTTTTAGGTATGAGCAGAAGAGGTTAAGGTATTTCTAAAGGTAACATATA
  GTCAAGAGTTTCCTCAAAATAGTTATTTGGAGAAGAATCAGAATGTCTGTGTATTTCTTGTCTGTTTCTA
  TGTTGTCTTATAGCTCTGACTAAATGTGTTTACCTATGCAAAAGATTTATTAAAGCATAGAAAAGGTGAA
  TGAATAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002395.3
• Summary: 'This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq, Jul 2008]'
• Locus ID: 4199