GATA4 (NM_002052) Human 3' UTR Clone

CAT#: SC215152

3`UTR clone of GATA binding protein 4 (GATA4) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: GATA4
• Synonyms: ASD2; TACHD; TOF; VSD1
• ACCN: NM_002052
• Insert Size: 1521 bp
• Sequence Data:
  Insert Sequence

  >SC215152 3'UTR clone of NM_002052
  The sequence shown below is from the reference sequence of NM_002052. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGGGACATAATCACTGCGTAATCTTCCCTCTTCCCTCCTCAAATTCCTGCACGGACCTGGGACTTGGAGG
  ATAGCAAAGAAGGAGGCCCTGGGCTCCCAGGGGCCGGCCTCCTCTGCCTGGTAATGACTCCAGAACAACA
  ACTGGGAAGAAACTTGAAGTCGACAATCTGGTTAGGGGAAGCGGGTGTTGGATTTTCTCAGATGCCTTTA
  CACGCTGATGGGACTGGAGGGAGCCCACCCTTCAGCACGAGCACACTGCATCTCTCCTGTGAGTTGGAGA
  CTTCTTTCCCAAGATGTCCTTGTCCCCTGCGTTCCCCACTGTGGCCTAGACCGTGGGTTTTGCATTGTGT
  TTCTAGCACCGAGGATCTGAGAACAAGCGGAGGGCCGGGCCCTGGGACCCCTGCTCCAGCCCGAATGACG
  GCATCTGTTTGCCATGTACCTGGATGCGACGGGCCCCTGGGGACAGGCCCTTGCCCCATCCATCCGCTTG
  AGGCATGGCACCGCCCTGCATCCCTAATACCAAATCTGACTCCAAAATTGTGGGGTGTGACATACAAGTG
  ACTGAACACTTCCTGGGGAGCTACAGGGGCACTTAACCCACCACAGCACAGCCTCATCAAAATGCAGCTG
  GCAACTTCTCCCCCAGGTGCCTTCCCCCTGCTGCCGGCCTTTGCTCCTTCACTTCCAACATCTCTCAAAA
  TAAAAATCCCTCTTCCCGCTCTGAGCGATTCAGCTCTGCCCGCAGCTTGTACATGTCTCTCCCCTGGCAA
  AACAAGAGCTGGGTAGTTTAGCCAAACGGCACCCCCTCGAGTTCACTGCAGACCCTTCGTTCACCGTGTC
  ACACATAGAGGGGTTCTGAGTAAGAACAAAACGTTCTGCTGCTCAAGCCAGTCTGGCAAGCACTCAGCCC
  AGCCTCGAGGTCCTTCTGGGGAGAGTGTAAGTGGACAGAGTCCTGGTCAGGGGGCAGGAGTGTCCCAAGG
  GCTGGCCCACCTGCTGTCTGTCTGCTCCTCCTAGCCCTTGGTCAGATGGCAGCCAGAGTCCCTCAGGACC
  TGCAGCCTCGCCCCGGCAGAAGTCTTTTGTCCAGGAGGCAAAAAGCCAGAGATTCTGCAACACGAATTCG
  AAGCAAACAAACACAACACAACAGAATTCCTGGAAAGAAGACGACTGCTAAGACACGGCAGGGGGGCCTG
  GAGGGAGCCTCCGACTCTGAGCTGCTCCGGGATCTGCCGCGTTCTCCTCTGCACATTGCTGTTTCTGCCC
  CTGATGCTGGAGCTCAAGGAGACTCCTTCCTCTTTCTCAGCAGAGCTGTAGCTGACTGTGGCATTACTAC
  GCCTCCCCACACGCCCAGACCCCTCACTCCAAAATCCTACTGGCTGTAGCAGAGAATACCTTTGAACCAA
  GATTCTGTTTTAATCATCATTTACATTGTTTTCTTCCAAAGGCCCCCTCGTATACCCTCCCTAACCCACA
  AACCTGTTAACATTGTCTTAAGGTGAAATGGCTGGAAAATCAGTATTTAAC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002052.3
• Summary: 'This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function, and is necessary for normal testicular development. Mutations in this gene have been associated with cardiac septal defects. Additionally, alterations in gene expression have been associated with several cancer types. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]'
• Locus ID: 2626