PPCDC (NM_021823) Human 3' UTR Clone

CAT#: SC215180

3`UTR clone of phosphopantothenoylcysteine decarboxylase (PPCDC) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PPCDC
• Synonyms: coaC; MDS018; PPC-DC
• ACCN: NM_021823
• Insert Size: 1503
• Sequence Data:
  Insert Sequence

  >SC215180 3'UTR clone of NM_021823
  The sequence shown below is from the reference sequence of NM_021823. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGAAGTCCTCTTCCAGCACAGTGGCTTCCAGCAGAGTTGACCTGGGATTTCTGTCATGGGTGTCCCTCTG
  TACTCAGAATGGGTTCAGGCCAAGTCGGTGAAGATGGATGTTGGCAAAATAGGAGGATACCCTCATTTGC
  TGAATGGGGGACCTGCTCTGAGCCTGCCCAGGGGCCAGGCCTGCTCCAGGTTAAACTGGACGGAAGGCCC
  AGGTCTCAGTTTCTTTCAACCAGGAGAGGCCGCTGCCTAGAGCCCCTCCCCACCTTTTCCTGGATGGGTG
  AGGCAAGCCAGGAGAGCAAGCAGTGTTGTCCTCACGGGAGGAGGACTGAGCGACTGGGAAAACTCGGCTC
  TACATCTCACCCAGAACGGCTTTTAGAAACACCACAGCTGGAGAGTCCTGGCTGAGCCTTGGGAGTTTCA
  GCTCTTTGGCGGGGTGCCCAGGTGCCATGCGATCAGCGAAGCCTGCGAGTTGGCAGGACTCTGAGGTTTC
  CTGCAGACCATGCCATGAGATTGAAGGTGCGGGGAAATAAAGAAAAATCACCATTTAGGAGACTCCATTC
  TTTCCCTACAACCCAGCTGTGGTCCCAGAGATCAGGGGGTGTTGCCAGGTGTGGCTGGGGAAGGGTCTGG
  GTTCACAACTCACCGGCACTCTTTAGTCCCCGTATAACATGGTGGTTAAGGATAAAGATCTGAAGCCAGA
  CAGCCCCTAGTTCCAATCCCAGCTCTGCCGCTTACTAGCAGTGGCAGTTTGGTTCAGGACTTAGCCTTCC
  TGACCTTCACTTACCTCAGCTATCTTGTTATCCTACCCTCAGGGAGTTGTTGAGAGGATTCAGTGAAATT
  TTGTGTGCAGAATACCCATGCCACTGAGTGCCTGGAACGTAGTAAATGTCAAATCAATGGAAGTTAAAAC
  TAGGCATGCATATATGCCAGAGAGCTTTCCTAGCATTTACTGCAATGCCCTGTCTCATTTTGGGAAAAAG
  GAAATCCTGGATGCTAAGTTGACACATCCCTTTATGCAAGTGACAGGTAAGCAGCCAGGAGGGTTGACCC
  TGCCCTGGGTCCCAGGCCTCTTGCCTGCTTCTTGGTTTCCTCTGTTCTCATCCTTTCAGCTTTGCTGGAC
  CTGAGGCAGGGGCCTGGGTGTCTGTGCCTCCCTACCCCCCAGTACACATGACCAAGGGAAGTAGATCCAG
  AGACACCATGCACTCTGGCCCAGAAACCCTCTGCTACCCAGCTTTTGACTTAATTGCCATTTGGTGTGGC
  TGCAGCGGGGAGGGCTTGTCTGCGTATTTGGAGCTGAGGCTGAGGCTGGCTTCCTGCCCTCTGGCTTCCA
  GGCCAGGTGTGAGGGACATCCCATGTGGGCTCTGAGAGCACTGAGAGTAGAGGTGCCTTGAGGGGACCAG
  GAAGCCTACTGGCTGGAATCAGTGCTCCAGCAGCCTCCACAGCCTTTATCTCAACAGCACTTGACTTCAC
  CAGGTGACCCTTTGGGATAAAATTGCCTTAGGG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_021823.3
• Summary: Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCDC (EC 4.1.1.36), one of the last enzymes in this pathway, converts phosphopantothenoylcysteine to 4-prime-phosphopantetheine (Daugherty et al., 2002 [PubMed 11923312]). [supplied by OMIM, Mar 2008]
• Locus ID: 60490