TMEM16A (ANO1) (NM_018043) Human 3' UTR Clone

CAT#: SC215210

3`UTR clone of anoctamin 1 calcium activated chloride channel (ANO1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ANO1
• Synonyms: DOG1; ORAOV2; TAOS2; TMEM16A
• ACCN: NM_018043
• Insert Size: 1569
• Sequence Data:
  Insert Sequence

  >SC215210 3'UTR clone of NM_018043
  The sequence shown below is from the reference sequence of NM_018043. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCAGCCATGCCTACCACGGGGGCGTCCTGTAGCTATGCCAGCGGGGCTGGGCAGGCCAGCCGGGCATCCT
  GACCGATGGGCACCCTCTCCCAGGGCAGGCGGCTTCCCGCTCCCACCAGGGCCCGGTGGGTCCTGGGTTT
  TCTGCAAACATGGAGGACCACTTTCTGATAGGACATTTTCCTTTCTTCTTTCTGTTTTCTTTCCCTTGTT
  TTTGCACAAAGCCATTATGCAGGGAATATTTTTTAATCTGTAGTATTCAAGATGAATCAAAATGATGGCT
  GGTAATACGGCAATAAGGTAGCAAAGGCAGGTGCTTTGCAGAAAGAATGCTTGGAAACTTGAGTCTCCCT
  AGAGGTGAAAAGTGAGCAGAGGCCCGTAGAAACCCTCCTCTGAATCCTCCTAATTCCTTAAGATAGATGC
  AAAATGGTAAGCCGAGGCATCGCGCAAAAGCTGGTGCGATGCTTCAGGGAAAATGGAAAACCCACGCAAG
  AATAATGATTGATTCCGGTTCCAAAAGGTGTCACCTACCTGTTTCAGAAAAGTTAGACTTTCCATCGCCT
  TTTCCTTCCATCAGTTGAGTGGCTGAGAGAGAAGTGCCTCATCCCTGAGCCACACAGGGGGCGTGGGAGC
  ATCCCAGTTATCCCTGGAAAGCTAGAAGGGGACAGAGGTGTCCCTGATTAAGCAGGAAACAGCACCCTTG
  GCGTCCCCAGCAGGCTCCCCACTGTCAGCCACACACCTGCCCCCATCACACCAAGCCGACCTCAGAGTTG
  TTCATCTTCCTTATGGGACAAAACCGGTTGACCAGAAAATGGGCAGAGAGAGATGACCTCGGAAGCATTT
  CCACAGATGGTGTCAGGGTTTCAAGAAGTCTTAGGGCTTCCAGGGGTCCCCTGGAAGCTTTAGAATATTT
  ATGGGTTTTTTTTTCAAATATCAATTATATGGTAGATTGAGGATTTTTTTTCTGTAGCTCAAAGGTGGAG
  GGAGTTTATTAGTTAACCAAATATCGTTGAGAGGAATTTAAAATACTGTTACTACCAAAGATTTTTATTA
  ATAAAGGCTTATATTTTGGTAACACTTCTCTATATTTTTACTCACAGGAATGTCACTGTTGGACAATTAT
  TTTAAAAGTGTATAAAACCAAGTCTCATAAATGATATGAGTGATCTAAATTTGCAGCAATGATACTAAAC
  AACTCTCTGAAATTTCTCAAGCACCAAGAGAAACATCATTTTAGCAAAGGCCAGGAGGAAAAATAGAAAT
  AAATTTGTCTTGAAGATCTCATTGATGTGATGTTACATTCCCTTTAATCTGCCAACTGTGGTCAAAGTTC
  ATAGGTGTCGTACATTTCCATTATTTGCTAAAATCATGCAATCTGATGCTTCTCTTTTCTCTTGTACAGT
  AAGTAGTTTGAAGTGGGTTTTGTATATAAATACTGTATTAAAAATTAGGCAATTACCAAAAATCCTTTTA
  TGGAAACCATTTTTTTAAAAAGTGAATGTACACAAATCCACAGAGGACTGTGGCTGGACATTCATCTAAA
  TAAATTTGAATATACGACACTTTTCTCAC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_018043.5
• Summary: Calcium-activated chloride channel (CaCC) which plays a role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development. [UniProtKB/Swiss-Prot Function]
• Locus ID: 55107