GLUD1 (NM_005271) Human 3' UTR Clone

CAT#: SC215287

3`UTR clone of glutamate dehydrogenase 1 (GLUD1) nuclear gene encoding mitochondrial protein for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: GLUD1
• Synonyms: GDH; GDH1; GLUD
• ACCN: NM_005271
• Insert Size: 1547 bp
• Sequence Data:
  Insert Sequence

  >SC215287 3'UTR clone of NM_005271
  The sequence shown below is from the reference sequence of NM_005271. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AAGCTGGTGTGACCTTCACATAGATGGATCATGGCTGACTTCCTCACTATCCTCTTCACATGTAACTTCT
  GCAGACCTATCACAAGTTTACATGTAACCACAGAAATCCCTTTCTCTCCTGACTCATTAATAATGGATAC
  CATTCTCAACAAGTCAATCCAAGTCAGCCCGTTAAGGAGAAAGAAATTAAGGTTAGCGGATCATGTACAA
  GCTGAGTGTGAAAGTAGAAATCACCTACACCAGAGAGCCATTTTGGTATTTTGCCTTTAAATAAAAAGCC
  TCCTTTATCTGGCTGTGCAGCCTTGCTCTGTGGCTTTTCCCAACACAATCAGTGCTAGTGCTGGGGAGGA
  ACAGTCAAGAGCAGTCAGTTGCTTGCTTATTTTTTCTGGATGAGTCTGGGACACACTGTAACTTTAACAC
  ATTTAAGAAGTAGGTGTGTGGCCTTTTCAGAAGGTGGCATGGTCCTCAAGTGAGTTCTTAGTATTTTATA
  TCAGCAAAATAATTCAATTTTGCAGGTTGCAAACAAATATAAAACCTGTTTCTGTTTATGAATATTATTC
  TTTTAGAATAGAATAAGTACATGCTGCTGTAATAAAATTGCCTTTAATCACTTAACAAGCCTAACCTTGA
  CTCAAACAGTGAATGCCTATAGAAATAATAAATGAAAAAAACTAGTATTTTTATATCATAAAACAATGTC
  ATTTATAGCTTATCATTCATGTATTGTCCAGCAGACATTAAAAGCCCTGTGGATAATTAAGTTATCTTCA
  TACCTGCAAAATGGTGGAGGCTATTTTCATTAAAACTGTCAGAATTTGCTTACTATAATTATGATACAGT
  CCAAAGAATGCAGTCACTTTTTATCATGTTAACTAATTGTTCTCTTTTGAAGATCTATGGTTGACTAATT
  AAACAATAATTCAAGTAGAGTGTCCCAGAAAAAAACCACTTGGGCTCCCTGTTTGGAGTCTGGCTGGCTC
  TGAGCATTGCCAATGGCCCCTACTCACCTGACTTTGTATCCTCTCCTTTTAGAGGCTTTGCATTCTGCAC
  CCAGCTTCACTAACAGTGGGCTGAAAACATCCTTGGGTTGAGTGTTTCATTTGGGAGTTATTTGGCCAGG
  GCCTTTTGAACAGTAGTGTCCCCATGAAGTGCTAGATAATATATGTGTAAGAGTCAGCTTTTTTTTTTTT
  TTTAACTCTAACACCCTTCAGAAATTTCTAACTACTTTGTAACTGCATGGCTTAACCTGGTGATAAAAGC
  AGTTATTAAAAGTCTACGTTTTCCAAAACTTACGTTTCTTTTCTGTGTTTTTACATGTGGTAGTTTCTCT
  TTTCATAAGTTATAATACTGCAATTGGATTTCTGAAATGTTTATAGCGACCACCTGTATAACATTTCCTT
  CCACTTTATTGTGAGCTGCCCAGATTTTATTCTTGAATTGTTTTTTTTTTTTTTGTTCGGTGCTTTACAC
  GTTCAGAGAAACTTCCCGAGTAACGAACTATAGAAATGATCCCTGAAAGCATAGTCTTTATTCTCGAATT
  ATTTTGT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005271.3
• Summary: 'This gene encodes glutamate dehydrogenase, which is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X. [provided by RefSeq, Jan 2016]'
• Locus ID: 2746