GLUD1 (NM_005271) Human 3' UTR Clone
CAT#: SC215287
3`UTR clone of glutamate dehydrogenase 1 (GLUD1) nuclear gene encoding mitochondrial protein for miRNA target validation
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Specifications
Product Data | |
Vector | pMirTarget |
Species | Human |
Transfection Reporter | RFP |
Assay Reporter | Luciferase |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Symbol | GLUD1 |
Synonyms | GDH; GDH1; GLUD |
ACCN | NM_005271 |
Insert Size | 1547 bp |
Sequence Data |
>SC215287 3'UTR clone of NM_005271
The sequence shown below is from the reference sequence of NM_005271. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon CAATTGGCAGAGCTCAGAATTCAAGCGATCGC AAGCTGGTGTGACCTTCACATAGATGGATCATGGCTGACTTCCTCACTATCCTCTTCACATGTAACTTCT GCAGACCTATCACAAGTTTACATGTAACCACAGAAATCCCTTTCTCTCCTGACTCATTAATAATGGATAC CATTCTCAACAAGTCAATCCAAGTCAGCCCGTTAAGGAGAAAGAAATTAAGGTTAGCGGATCATGTACAA GCTGAGTGTGAAAGTAGAAATCACCTACACCAGAGAGCCATTTTGGTATTTTGCCTTTAAATAAAAAGCC TCCTTTATCTGGCTGTGCAGCCTTGCTCTGTGGCTTTTCCCAACACAATCAGTGCTAGTGCTGGGGAGGA ACAGTCAAGAGCAGTCAGTTGCTTGCTTATTTTTTCTGGATGAGTCTGGGACACACTGTAACTTTAACAC ATTTAAGAAGTAGGTGTGTGGCCTTTTCAGAAGGTGGCATGGTCCTCAAGTGAGTTCTTAGTATTTTATA TCAGCAAAATAATTCAATTTTGCAGGTTGCAAACAAATATAAAACCTGTTTCTGTTTATGAATATTATTC TTTTAGAATAGAATAAGTACATGCTGCTGTAATAAAATTGCCTTTAATCACTTAACAAGCCTAACCTTGA CTCAAACAGTGAATGCCTATAGAAATAATAAATGAAAAAAACTAGTATTTTTATATCATAAAACAATGTC ATTTATAGCTTATCATTCATGTATTGTCCAGCAGACATTAAAAGCCCTGTGGATAATTAAGTTATCTTCA TACCTGCAAAATGGTGGAGGCTATTTTCATTAAAACTGTCAGAATTTGCTTACTATAATTATGATACAGT CCAAAGAATGCAGTCACTTTTTATCATGTTAACTAATTGTTCTCTTTTGAAGATCTATGGTTGACTAATT AAACAATAATTCAAGTAGAGTGTCCCAGAAAAAAACCACTTGGGCTCCCTGTTTGGAGTCTGGCTGGCTC TGAGCATTGCCAATGGCCCCTACTCACCTGACTTTGTATCCTCTCCTTTTAGAGGCTTTGCATTCTGCAC CCAGCTTCACTAACAGTGGGCTGAAAACATCCTTGGGTTGAGTGTTTCATTTGGGAGTTATTTGGCCAGG GCCTTTTGAACAGTAGTGTCCCCATGAAGTGCTAGATAATATATGTGTAAGAGTCAGCTTTTTTTTTTTT TTTAACTCTAACACCCTTCAGAAATTTCTAACTACTTTGTAACTGCATGGCTTAACCTGGTGATAAAAGC AGTTATTAAAAGTCTACGTTTTCCAAAACTTACGTTTCTTTTCTGTGTTTTTACATGTGGTAGTTTCTCT TTTCATAAGTTATAATACTGCAATTGGATTTCTGAAATGTTTATAGCGACCACCTGTATAACATTTCCTT CCACTTTATTGTGAGCTGCCCAGATTTTATTCTTGAATTGTTTTTTTTTTTTTTGTTCGGTGCTTTACAC GTTCAGAGAAACTTCCCGAGTAACGAACTATAGAAATGATCCCTGAAAGCATAGTCTTTATTCTCGAATT ATTTTGT ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG |
Restriction Sites | SgfI-MluI |
OTI Disclaimer | Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs). |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. |
Reference Data | |
RefSeq | NM_005271.3 |
Summary | 'This gene encodes glutamate dehydrogenase, which is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X. [provided by RefSeq, Jan 2016]' |
Locus ID | 2746 |
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