Calbindin (CALB1) (NM_004929) Human 3' UTR Clone

CAT#: SC215316

3`UTR clone of calbindin 1 28kDa (CALB1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CALB1
• Synonyms: CALB; D-28K
• ACCN: NM_004929
• Insert Size: 1587 bp
• Sequence Data:
  Insert Sequence

  >SC215316 3'UTR clone of NM_004929
  The sequence shown below is from the reference sequence of NM_004929. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TGTACCGAACGGATCTTGCTCTTATTCTCTGTGCTGGGGATAACTAGAGTTGGTGGCCGCAACCACTTGC
  TAGTGATACACTGTATCTAAAAAATAACTGTGCACTATAAGGGAGTAGGCTGTATTTTCTTTTATATCTG
  TAAATTTAACTGCATATAGATAATTATCCAGGATGTGTGGCTCATTCTTTTCAGCTTGTTTCTATACTGT
  TTGTAATATACAGTTTTTGTAACCATATGATTGAAAAGAAGAAAGTCTATGCTTAGGCCAGTCAGTACAC
  CCAATTTTAAAAAATAACATATTCTTGCTTTCACAAATATAGTTGAACAAGATTTCCCTAAAAATTCCAC
  CAGGATTAATCTCTAAAATTCTAGTCTCTGATTTGCAAATGCACATTTGTCACTGAATAATGGAATTATG
  TATAACAAGCCAAACATTCTTATTTTAGACAACCATAGAACTGTCCCACAAAATATTTCTAAGCTTATTT
  CTAACTATTAGGAGGAATGTGCTTTTCCATCTAAAATACTCACCAAAATATAGTTAATTGTGGCTTTATG
  AAGTTAACAGTCTCATTACAGATTTAGTTTACCAATCAACAGCATGTCTACTGCTTGGATCCATACAAAA
  CTATCGGTTCAAGTTGATGTGACAAGGGAAGGGAGCACCAGATGACACATAAATCTGTCTGATTCTATGC
  CTGTATTTCCAACAAACTTACTGTCAGAGAATATGACCTAAATCCATTTTCTAAACTGTTTTCATGTGTT
  GCAAATTATTCTAGTCAACTGCTGTTTTATGTCATACTCTGTGTAATCTCTGATTAAATTTAATATACTG
  CATATCCTGGTGTCTAGTTTGCATACTTCCTGGATTTTCTTTCTATGTAGAACTGTTCATTTCCACCAAG
  GGTATCTGCTGCCTCTGAAAATATTTTTTTCTAGCTATAACAACTCTATTTTTTACTACATAATTAAATT
  TTAATGTAAAATTCATAGCATCCTGATTATTGAATGTTATATCATCAATACTTTTGTGTATTCTGTGGAT
  TCTATATTTCATATTGAGATCAGCATTCAAAATAGTTCTATTTCTATCTGCAAATAGTTTCAAATGAGTT
  TAAAAAAATAACATCTGAAAAGAAATGCTAATGTAATCATTTATCTTATCTAGCAAGAAGATTCTAAAAC
  ATTCTTTAACATACATCTAAGTCAGTTTCACATATTTGTAGCTAGAATATCCTATACTGGTTATAGTTGA
  TATGTAACAGTTGGTGATTTTAGATTTCTTTGATTGTGAAACAGGGAGCTATGAGAGATGTGTCCATGTG
  AAATTTACAGTTACTGCCTAGGAGTTAATGATCGTTCTGGGTCAGCTTGAATGTCCCCATTCTATAAATT
  CAACACTTATTTTCTGAATTCATAAAAATAACCAAAAAATGTGAGCTATAATGTTTCCCTCAAGAACAAA
  CAGAAACGAGATTTGCCAAAAACTAAAATTCAACAAATGATGTTGAGTGGGAGATTGGCTTTGCCTTTAG
  CGTGTAAATGGAAGCACTGCCATTAGACTGAATTTAACTACTAAGAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004929.2
• Summary: 'The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease. [provided by RefSeq, Jan 2015]'
• Locus ID: 793