B3GALT2 (NM_003783) Human 3' UTR Clone

CAT#: SC215324

3`UTR clone of UDP-Gal:betaGlcNAc beta 13-galactosyltransferase polypeptide 2 (B3GALT2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: B3GALT2
• Synonyms: beta3Gal-T2; BETA3GALT2; GLCT2
• ACCN: NM_003783
• Insert Size: 1569
• Sequence Data:
  Insert Sequence

  >SC215324 3'UTR clone of NM_003783
  The sequence shown below is from the reference sequence of NM_003783. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CAGGTATCGCCACCGTAAACTACATTAGAAAAGACAATTTTTTTTCAAATGTGCAATTTGTAAATATTGC
  TAAAAGCATGTATAGTTAGAACTGATTACATCCGTAGGACAAGTTTTAGTTAAAACTCATCACATAAAGA
  AATTCAAGAAGTATTTTTTTAATTTCTGAAGAAGTTAATTCTTAAAACTATAACATTATATAACAAAAAA
  GGTTTCCCAAAACAATCTATTTAAAAAACTGTATAAGGAGATTCTGTGTATTAACATGCAATAACAAGCA
  TGCATAAATCAATGGTTCAAGTCTTCTGTTAGGGGCCAATAAAATGTATCTGCATATGTTTTCCACATAA
  ATTTTAATTCAAGAAATGACAGTCAAAAGATCCTTCATTTTAGATTAAGCTTTTCATTTTAATATATAAT
  TTAATGTAAATAAAACATCACTATCAATTTTAAGGAAACTTTTTAATTGTGCAAAGGATAAATTTTTTGA
  CCTATTTTAGGGTTCTAAATGCAATAAGATTTAGTTGAGTTATTCCACAAACACATTATAAAGTTCAGAT
  GTTTCATCAATGCAGTTCTCACGAAAGTATTTACTTTTTAAAAATAACTGAGATATTATTTTAAATTTCT
  TTTATTAATACTTTCTTTTATTAATATATGGGGGAAAATTATTTTGACATGACGTGGTAAAATGTGAAAA
  ACTAATGTGTCTCAGGCTCAAGTTTTTATAGTTATTAAATGTTTCAAAATAGACAAGTTTTGTTTCCTCA
  TTGATGTTAAGAACCAAACTCCTATTTCAATGAGTTATTGGATTAGACCAATTACTGCACTCTTAAACAG
  CACCACCATTTAATTTCATGTATATCTAACTTCGAATATATCTGTAAAGATAATCGAAGCAAAAGTAATC
  ACTTAAAGGCACAAATAGGATGTACTGTTGAAAAAGATAAAGAGTGCAGGTGCAGTTTCATTCAACACAT
  TTTTAAGATGCATGTCTGCCAAAATGCAACATACGGGAAGTTTATTTCCTGACAGCAGGTGTACACATGC
  CAACACTTAATCATTTTATGGCACCTATTTCTTTCTTGGAGTGCCAAGTTTGCAAACCTGCAGTTTTTAA
  TTTGGTAGATGACAAATATTCTGAATCACCAATTAAAAACCTTTTTGGGAGGGATGGGGAAAACTACAAA
  CGTTTGACAAACACAATTCTAGGATGAACAATGTATACAATGCACTTTTATGAAGTTTTTAAAAATAAAG
  GAAAACAAAAAACTTTACTGAGTGTTATAAGTATTTTCACAATAATTTTGTTTGACTCCATCATGAATTT
  TAAAATCAAGAAATACCAACCTACTAACTAAAATAGTGTTTTTATTCCATACAAGTATGTAAATCTGTCA
  ATCTTAATTTAGTCAGAATGTTTCTTAATTCTAATAACAATCATTTTTACCTCTTATTCTATTATTAAGG
  GATAAATCACCAGAAGAGAATTGTAACATTTGTGAAACTTGAAAACACTAAAACTGTTTAAGTGCTTATC
  TCCATTTCAAATCAGTAGCCTAAGTGTTC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_003783.3
• Summary: This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5). This gene encodes a protein that functions in N-linked glycoprotein glycosylation and shows strict donor substrate specificity for UDP-galactose. [provided by RefSeq, Jul 2008]
• Locus ID: 8707