CREM (NM_182721) Human 3' UTR Clone

CAT#: SC215330

3`UTR clone of cAMP responsive element modulator (CREM) transcript variant 8 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CREM
• Synonyms: CREM-2; hCREM-2; ICER
• ACCN: NM_182721
• Insert Size: 1595 bp
• Sequence Data:
  Insert Sequence

  >SC215330 3'UTR clone of NM_182721
  The sequence shown below is from the reference sequence of NM_182721. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGAACTCAAGGCCCTCAAAGATCTTTATTGCCATAAAGTAGAGTAACTGTCTTTGACTTGGACCTTGTTT
  ACTCTAATCAAGGCAGGAGATGCAGCAGTCCTACTTATTGCCATGTGGACTTGTGGGAAGGACACGTGTG
  ACCCTTAAGAATCCAGTTTGGATTAGTGTTTGAAATTGAATTGGGAATGTTGTTCCAGGATGTGGAATGC
  AGCCGTGATCACACTTACCGAGCTTACTTTGATCTGTTTGTCAATAGCATGCAAAAAATGCTTTGTTTGC
  CCTTTGCTTCTGCTTTTTTTCAGGGAAGCTGCCAAAGAATGTCGACGTCGAAAGAAAGAATATGTAAAAT
  GTCTGGAGAGCCGAGTTGCAGTGCTGGAAGTCCAGAACAAGAAGCTTATAGAGGAACTTGAAACCTTGAA
  AGACATTTGTTCTCCCAAAACAGATTACTAGAAATATTTAACTATGAACTGAAGGCAGCATGTATAGTTG
  CTTTTGAAGGAATACAATATATAGCTGGCAAGAATGGTGGCTTCTTTTCTTTGTATCATTCATCTTCTTC
  TTTAATCACTTAACATTCCTAAAATGCTTCACTGTACGTAGTTAAGTCGTAGCTATAACTTCAAATTTTT
  TAAAAGAGACAAACTGTAAAAAATGTGTGTATTCTTAAAATGCAATATTTGTAAGGCTTGTTCCAATGCC
  ACATACTTGCAGCTCCCATTCTATGTGTCATCAATAGTGTCCTATGCAATAAAATTATTTGCAGGTCTTT
  AAATCATTTTAGGAAAGGATGATCAAAAATAATGCATCCAGCAGTACAATAAAAGTAAACCACAAAAAAA
  TACCTCAGGAAAGAATAGAAAGAAAGTCTATCTAATGACATGCCTATATGAGAAGAATAGCCTAGATATG
  AATATATGGCATTTGCAGATTTTTATATTAGTTGCTTTGTTAAAAAAAAAAAGATTGTATTGCTGTCCTT
  GAATGCCATAGTCAAAGAGAGTTTTTAATAGAACCATGTTGGTTGCACTTTGTAGTGTTTGGTGCTCATT
  TAAATATCTGAACATTTACTACAGTTTTTAACTCTACTGTGTAATATAAAAGATCTTGCAGAAGTTCTTA
  GTGTTGGTTTAATATTACCTAATGAAAGTGATGACATATTTTTTATATCTGGAATGAGCCTGTTGGGATC
  GCATTGCATACCTTCGGGGTACAAGTCAGTTTCTACACTGGGTGCTGATCCTTGCACGCGCCCTTTCTAC
  CATCTCACGGTGGGGATGGCCGCAGGGCTGTGCACCCAGAAGAAGATGGCTGTTAGCGTTTCGGCCTTCA
  TAATGGCCTGAGACTTTCTTTCTGTAGGTGGTCTGGAGCTGTCCGGCTGGTGGCCCCCTATTTTGCCATT
  TAGCGAACAACCACAGGAATTTTAAAAACAAAAACATCCCAAGATTTTTTCATTTCAAAATGCTTCAAAG
  TCCACATTAGATCAGATACTCCGCTGTCGGCACATTCAGCTGAGGTTCATTACAATCGAGACTGCAATGT
  GATCTATGTTTCATCTTGTTTTTATAATAAAAAGCTTCAGGGAACAAGCCCAAAG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_182721.1
• Summary: 'This gene encodes a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is an important component of cAMP-mediated signal transduction during the spermatogenetic cycle, as well as other complex processes. Alternative promoter and translation initiation site usage allows this gene to exert spatial and temporal specificity to cAMP responsiveness. Multiple alternatively spliced transcript variants encoding several different isoforms have been found for this gene, with some of them functioning as activators and some as repressors of transcription. [provided by RefSeq, Jul 2008]'
• Locus ID: 1390