IDH3A (NM_005530) Human 3' UTR Clone

CAT#: SC215392

3`UTR clone of isocitrate dehydrogenase 3 (NAD+) alpha (IDH3A) nuclear gene encoding mitochondrial protein for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: IDH3A
• Synonyms: H-IDH alpha; isocitrate dehydrogenase (NAD+) alpha chain; isocitrate dehydrogenase 3 (NAD+) a; isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; isocitric dehydrogenase; NAD(H)-specific isocitrate dehydrogenase alpha subunit; NAD+-specific ICDH
• ACCN: NM_005530
• Insert Size: 1552 bp
• Sequence Data:
  Insert Sequence

  >SC215392 3'UTR clone of NM_005530
  The sequence shown below is from the reference sequence of NM_005530. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGAGGAAATCTGTCGCCGAGTAAAAGATTTAGATTAACACTTCTACAACTGGCATTTACATCAGTCACTC
  TAAATGGACACCACATGAACCTCTGTTTAGAATACCTACGTATGTATGCATTGGTTTGCTTGTTTCTTGA
  CAGTACATTTTTAGATCTGGCCTTTTCTTAACAAAATCTGTGCAAAAGATGCAGGTGGATGTCCCTAGGT
  CTGTTTTCAAAGAACTTTTTCCAAGTGCTTGTTTTATTTATTAAGTGTCTACCTGGTAAATGTTTTTTTT
  GTAAACTCTGAGTGGACTGTATCATTTGCTATTCTAAACCATTTTACACTTAAGTTAAAATAGTTTCTCT
  TCAGCTGTAAATAACAGGATACAGAATTAACAAGAGAAAATGTCTAACTTTTTAAGAAAAACCTTATTTT
  CTTCGGTTTTTGAAAAACATAATGGAAATAAAACAGGATATTGACATAATAGCACAAAATGACACTCTTC
  TAAAACTAAATGGGCACAAGAGAATTTTCCTGGGAAAGTTCACATCAAAAAGAGTGAATGTGGTATATTT
  CTAAATGATATGGAAAATAGAGACAGATTTGTCCTTTACAGAAATTACTGAGTGTGAATAAAAACTTCAG
  ATCCAAGAAATATATAATGAGAGATATAATTTTTGTTAATAAGACAAAGGTAATATATTGGATACAAAGA
  CACAAATGTATTGTGTGTTCAATTATTTTGTTGTCTTGAGATTTAATATTCTTTCCAAGAGCTTTTAATG
  AAGCAGAGAGCTAGTACTTCATTTTCACTGGATACATTTTCAGCATCATGAGTTGTCACAGCCTCTGAGC
  CCCTGATCTGAAGCCAGAAGGGCTGAGTGTATTGTAAACTTATTCTTGCATGTTGCTGTCTGGGAATGGA
  CCACACTACAGCAGGTAGTTCTGGGGGCGATACTGCCGAAAGGCCCGAACACATGTATTTTGGCTGCAAT
  TGAGGAACTTGGGATGCTATTAATTTTGTATTTCAGCAACTGCCCCTTCTCCTATCCCAAAGCACCAATT
  ACTGCCCTCTGCCTCAGCAGTACCAGTATAAGATGACATTCCAAAGACTGGAGGCAACTCAGCCTGAGTT
  AATTCACAAAATTATGCCATGCTGGGGCTTGAGCTTGAGCTTGGGCTTAGGCTTGGGCTCAGCTTTTGAC
  CCTCAGGCATCTCCTTTCCCTTCCTGTCTTCCTCTCCCTTCTCCTCTGCTGCAGCATGATTTTCTTAATC
  TTCAGACACTCACTATTTTCATGAACAGTTACCCTCTGTCCCCACAACCAAAGACAACTCATGGCCTCCT
  TTGGCCCTTGTGTAACATTGCAAACCTGTGGCTTTGCAAAATGTACCCAGGTCACAAGGGGATTTTTTTT
  TTTTTAGCAATGATATCCCTGTCTGGGTCACTTTTTAAGCTTGTAACCGCCCCCCCAGACTTATAATCTT
  AAATGTATTTTCCTTTGTTTAAGCTGCTGCTTCCTCTGTTTCATTGGATTGTGCCAGTTATCAGTGGCTC
  TTGGGTTCAAAG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005530.2
• Summary: 'Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. [provided by RefSeq, Jul 2008]'
• Locus ID: 3419