MMP14 (NM_004995) Human 3' UTR Clone

CAT#: SC215401

3`UTR clone of matrix metallopeptidase 14 (membrane-inserted) (MMP14) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: MMP14
• Synonyms: MMP-14; MMP-X1; MT-MMP; MT-MMP 1; MT1-MMP; MT1MMP; MTMMP1; WNCHRS
• ACCN: NM_004995
• Insert Size: 1570 bp
• Sequence Data:
  Insert Sequence

  >SC215401 3'UTR clone of NM_004995
  The sequence shown below is from the reference sequence of NM_004995. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TGCTCTACTGCCAGCGTTCCCTGCTGGACAAGGTCTGACGCCCACCGCCGGCCCGCCCACTCCTACCACA
  AGGACTTTGCCTCTGAAGGCCAGTGGCAGCAGGTGGTGGTGGGTGGGCTGCTCCCATCGTCCCGAGCCCC
  CTCCCCGCAGCCTCCTTGCTTCTCTCTGTCCCCTGGCTGGCCTCCTTCACCCTGACCGCCTCCCTCCCTC
  CTGCCCCGGCATTGCATCTTCCCTAGATAGGTCCCCTGAGGGCTGAGTGGGAGGGCGGCCCTTTCCAGCC
  TCTGCCCCTCAGGGGAACCCTGTAGCTTTGTGTCTGTCCAGCCCCATCTGAATGTGTTGGGGGCTCTGCA
  CTTGAAGGCAGGACCCTCAGACCTCGCTGGTAAAGGTCAAATGGGGTCATCTGCTCCTTTTCCATCCCCT
  GACATACCTTAACCTCTGAACTCTGACCTCAGGAGGCTCTGGGCACTCCAGCCCTGAAAGCCCCAGGTGT
  ACCCAATTGGCAGCCTCTCACTACTCTTTCTGGCTAAAAGGAATCTAATCTTGTTGAGGGTAGAGACCCT
  GAGACAGTGTGAGGGGGTGGGGACTGCCAAGCCACCCTAAGACCTTGGGAGGAAAACTCAGAGAGGGTCT
  TCGTTGCTCAGTCAGTCAAGTTCCTCGGAGATCTGCCTCTGCCTCACCTACCCCAGGGAACTTCCAAGGA
  AGGAGCCTGAGCCACTGGGGACTAAGTGGGCAGAAGAAACCCTTGGCAGCCCTGTGCCTCTCGAATGTTA
  GCCTTGGATGGGGCTTTCACAGTTAGAAGAGCTGAAACCAGGGGTGCAGCTGTCAGGTAGGGTGGGGCCG
  GTGGGAGAGGCCCGGGTCAGAGCCCTGGGGGTGAGCCTGAAGGCCACAGAGAAAGAACCTTGCCCAAACT
  CAGGCAGCTGGGGCTGAGGCCCAAAGGCAGAACAGCCAGAGGGGGCAGGAGGGGACCAAAAAGGAAAATG
  AGGACGTGCAGCAGCATTGGAAGGCTGGGGCCGGGCAGGCCAGGCCAAGCCAAGCAGGGGGCCACAGGGT
  GGGCTGTGGAGCTCTCAGGAAGGGCCCTGAGGAAGGCACACTTGCTCCTGTTGGTCCCTGTCCTTGCTGC
  CCAGGCAGCGTGGAGGGGAAGGGTAGGGCAGCCAGAGAAAGGAGCAGAGAAGGCACACAAACGAGGAATG
  AGGGGCTTCACGAGAGGCCACAGGGCCTGGCTGGCCACGCTGTCCCGGCCTGCTCACCATCTCAGTGAGG
  GGCAGGAGCTGGGGCTCGCTTAGGCTGGGTCCACGCTTCCCTGGTGCCAGCACCCCTCAAGCCTGTCTCA
  CCAGTGGCCTGCCCTCTCGCTCCCCCACCCAGCCCACCCATTGAAGTCTCCTTGGGCCACCAAAGGTGGT
  GGCCATGGTACCGGGGACTTGGGAGAGTGAGACCCAGTGGAGGGAGCAAGAGGAGAGGGATGTCGGGGGG
  GTGGGGCACGGGGTAGGGGAAATGGGGTGAACGGTGCTGGCAGTTCGGCTAGATTTCTGTCTTGTTTGTT
  TTTTTGTTTTGTTTAATGTATATTTTTATT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004995.2
• Summary: 'Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq, Jul 2008]'
• Locus ID: 4323