SDF1 (CXCL12) (NM_199168) Human 3' UTR Clone

CAT#: SC215420

3`UTR clone of chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) (CXCL12) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CXCL12
• Synonyms: IRH; PBSF; SCYB12; SDF1; TLSF; TPAR1
• ACCN: NM_199168
• Insert Size: 1607 bp
• Sequence Data:
  Insert Sequence

  >SC215420 3'UTR clone of NM_199168
  The sequence shown below is from the reference sequence of NM_199168. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GTGGATTCAGGAGTACCTGGAGAAAGCTTTAAACAAGTAAGCACAACAGCCAAAAAGGACTTTCCGCTAG
  ACCCACTCGAGGAAAACTAAAACCTTGTGAGAGATGAAAGGGCAAAGACGTGGGGGAGGGGGCCTTAACC
  ATGAGGACCAGGTGTGTGTGTGGGGTGGGCACATTGATCTGGGATCGGGCCTGAGGTTTGCCAGCATTTA
  GACCCTGCATTTATAGCATACGGTATGATATTGCAGCTTATATTCATCCATGCCCTGTACCTGTGCACGT
  TGGAACTTTTATTACTGGGGTTTTTCTAAGAAAGAAATTGTATTATCAACAGCATTTTCAAGCAGTTAGT
  TCCTTCATGATCATCACAATCATCATCATTCTCATTCTCATTTTTTAAATCAACGAGTACTTCAAGATCT
  GAATTTGGCTTGTTTGGAGCATCTCCTCTGCTCCCCTGGGGAGTCTGGGCACAGTCAGGTGGTGGCTTAA
  CAGGGAGCTGGAAAAAGTGTCCTTTCTTCAGACACTGAGGCTCCCGCAGCAGCGCCCCTCCCAAGAGGAA
  GGCCTCTGTGGCACTCAGATACCGACTGGGGCTGGGCGCCGCCACTGCCTTCACCTCCTCTTTCAACCTC
  AGTGATTGGCTCTGTGGGCTCCATGTAGAAGCCACTATTACTGGGACTGTGCTCAGAGACCCCTCTCCCA
  GCTATTCCTACTCTCTCCCCGACTCCGAGAGCATGCTTAATCTTGCTTCTGCTTCTCATTTCTGTAGCCT
  GATCAGCGCCGCACCAGCCGGGAAGAGGGTGATTGCTGGGGCTCGTGCCCTGCATCCCTCTCCTCCCAGG
  GCCTGCCCCACAGCTCGGGCCCTCTGTGAGATCCGTCTTTGGCCTCCTCCAGAATGGAGCTGGCCCTCTC
  CTGGGGATGTGTAATGGTCCCCCTGCTTACCCGCAAAAGACAAGTCTTTACAGAATCAAATGCAATTTTA
  AATCTGAGAGCTCGCTTTGAGTGACTGGGTTTTGTGATTGCCTCTGAAGCCTATGTATGCCATGGAGGCA
  CTAACAAACTCTGAGGTTTCCGAAATCAGAAGCGAAAAAATCAGTGAATAAACCATCATCTTGCCACTAC
  CCCCTCCTGAAGCCACAGCAGGGTTTCAGGTTCCAATCAGAACTGTTGGCAAGGTGACATTTCCATGCAT
  AAATGCGATCCACAGAAGGTCCTGGTGGTATTTGTAACTTTTTGCAAGGCATTTTTTTATATATATTTTT
  GTGCACATTTTTTTTTACGTTTCTTTAGAAAACAAATGTATTTCAAAATATATTTATAGTCGAACAATTC
  ATATATTTGAAGTGGAGCCATATGAATGTCAGTAGTTTATACTTCTCTATTATCTCAAACTACTGGCAAT
  TTGTAAAGAAATATATATGATATATAAATGTGATTGCAGCTTTTCAATGTTAGCCACAGTGTATTTTTTC
  ACTTGTACTAAAATTGTATCAAATGTGACATTATATGCACTAGCAATAAAATGCTAATTGTTTCATGGTA
  TAAACGTCCTACTGTATGTGGGAATTTATTTACCTGAAATAAAATTCATTAGTTGTTAGTGATGGAG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_199168.3
• Summary: 'This antimicrobial gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]'
• Locus ID: 6387