MAPKAP Kinase 2 (MAPKAPK2) (NM_032960) Human 3' UTR Clone

CAT#: SC215446

3`UTR clone of mitogen-activated protein kinase-activated protein kinase 2 (MAPKAPK2) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: MAPKAPK2
• Synonyms: MAPKAP-K2; MK-2; MK2
• ACCN: NM_032960
• Insert Size: 1582
• Sequence Data:
  Insert Sequence

  >SC215446 3'UTR clone of NM_032960
  The sequence shown below is from the reference sequence of NM_032960. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGCGGAAGAAAGCTCGGGCCCTGGAGGCTGCGGCTCTGGCCCACTGAGCCACCGCGCCCTCCTGCCCACG
  GGAGGACAAGCAATAACTCTCTACAGGAATATATTTTTTAAACGAAGAGACAGAACTGTCCACATCTGCC
  TCCTCTCCTCCTCAGCTGCATGGAGCCTGGAACTGCATCAGTGACTGAATTCTGCCTTGGTTCTGGCCAC
  CCCAGAGTGGGAGAGGCTGGGAGGTTGGGAGGCTGTGGAGAGAAGTGAGCAAGGTGCTCTTGAACCTGTG
  CTCATTTTGCAATTTTATCAGTAATTTGACTTAGAGTTTTTACGAAACCTCTTTTGTTGTCCTTGCCCCA
  CTCCTCTCCACCAGACGCCTTCCTCTCTGGATACTGCAAAGGCTTGTGGTTTGTTAGAGGGTATTTGTGG
  AAACTGTCATAGGGATTGTCCCTGTGTTGTCCCATCTGCCCTCCCTGTTTCTCCACAACAGCCTGGGGTT
  GTCCCCGCTGGCTCACGCGTTCTGGGAGCTCAAGGCCACCTTGGAGGAGGATGCCACGCACTTCCTCTCT
  CGGAGCCCTCAGACATCTCCAGTGTGCCAGACAAATAGGAGTGAGTGTATGTGTGTGTGTGTGTGTGTGT
  GTGTGCACACGTGTGTATGAGTGCGCAGATCTGTGCCTGGGATCGTGCATTTGAGGGGCCAGGGGCAGGC
  AGGGCTGCAGAGGGAGACGGCCCTGCTGGGGCTTAGGAACCTTCTCCCTTCTTGGGTCTGCCCTGCCCAT
  ACTGAGCCTGCCAAAGTGCCTGGGAAGCCCACCCAGATTCTGAAACAGGCCCTCTGTGGCCTGTCTCTAT
  TAGCTGGGTTCCGGGAGGCAGAGAGGAGTGACCGGGCACTGGCACTGCGATCAGGAAGACTGGACCCCCA
  GCCCCCAGGGCCCCCCTCCCCCCACTTAGTGCTGGTCCTAGGTCCTCTGAGGCACTCATCTACTGAATGA
  CCTCTCTACTTCCCCTTCTTGCCATTATTAACCCATTTTTGTTTATTTTCCTTAAATTTTTAGCCATTTC
  TCCATGGGCCACCGCCCAGCTCATGTAGGTGAGCCTGGGCAGCTTCTGTTGGCAGAGCTTTTGCATTTCC
  TGTGTTTGTCCTGGGTTCTGGGGCATCAGCCAGCTACCCCTTGTGGGCAAAGGCAGGGCCACTTTTGAAG
  TCTTCCCTCAGATTTCCATTGTGTGGCCTGGTGGGTCAGGGGGAGTCTTTGCACCAAAGATGTCCTGACT
  TTGCCCCCTTGCCCATCAGCCATTTGCCATCACCCCAAACAACTCAGCTTCGGGGCCGGTGAGGGGAGGG
  GCCTCCCCCAGCACAGATGAGGAGCAGCTGGGGTAGGCTGTCTGTGCCATGGCCCCCCACTCCCCCTTCC
  CTTGGAGGGAGAGGTGGCAGGAATACTTCACCTTTCCTCTCCCTCAGGGGCAGGTGGTGGAGGGGCGCCC
  AGGGTCGTCTTTGTGTATGGGGGAAGGCGCTGGGTGCCTGCAGCGCCTCCCTTGTCTCAGATGGTGTGTC
  CAGCACTCGATTGTTGTAAACTGTTGTTTTGTATGAGCGAAA
  
  CGGACCGTTACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-RsrII
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_032960.2
• Summary: This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Locus ID: 9261