Chimaerin 2 (CHN2) (NM_001039936) Human 3' UTR Clone

CAT#: SC215552

3`UTR clone of chimerin (chimaerin) 2 (CHN2) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CHN2
• Synonyms: ARHGAP3; BCH; CHN2-3; RHOGAP3
• ACCN: NM_001039936
• Insert Size: 1605 bp
• Sequence Data:
  Insert Sequence

  >SC215552 3'UTR clone of NM_001039936
  The sequence shown below is from the reference sequence of NM_001039936. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GATTTTAATAGAAAACGAAGACGTTTTATTCTAATCCATCAGGGAAATGAGCTGAATGGCCCCAGCACCA
  TCCAAGTTGACACAGCTAAAGGAATAAAAACATTTCTTACCACTTGATTTGTTTTCCAAGCAAGTGCTAG
  AATTTGCTGGACTGCAGAGGATCGCTGAGTGGGGTACTGTGTCTCATAGACATGCGCCACCTCCACGTGA
  GAACAAGGGTGAAGGTGAGGGAAGCCCCTCACCTTGGGTCTTTTGCTGTGCCTCCTATGTATGTCTGGTT
  TGCTGGAAGAGTGATTAATACATCTTTAATTTATTAAAAAACAATGTAGACCTTTAAACTTCAGTCTTAT
  TGGTAATAAAAGGGAACTTAATTCATACAGGTACTTGATACAGTTATACATTTTCCACTTACAAAAAGAA
  GACAATTCTGTTAAATGAAACGTGTATCGTAAAATGTATTTTTATTTTACCCACGAGAATGTTGTTATTT
  TTAGCAAATAGAACTCAATGCAGTGCATTGGTTATTACCCTGTGTACCTTGTCCCTCATTTTGCTGTGAC
  ACCCTGAAAAAGCTGACCACAAATGCAGTATTATCTTGACATACCTCTGTCCTCCTCAGTGCTTTTTAAT
  GAAATTTCACCTGCCCCTTGTGTTCCTGCTTTGGATAGTTTTTGCTCTTAAGCATTGGCATTCTTCTATT
  GTATGGTATGTATTTATAGCAATTGTAGGATGGTCTGAAATGTCCACACACTTTCTATGCATACAAATTT
  TGTGAATTCCCAAAGTATGCTTTGGAATTGGTCATAGTCTTTTCGTATCATGAGTGTGTATCCCAGAAGA
  TACAGAATGCGGTCATTTTACCTGAAATTATTTGAGAACACTGGATGTATCTGGTCTGTGTAAAAAGTTA
  GCACTTACCCTCTTTTCTTCCTGGCCAAGCCCTCTGAATTCATCTGTTAATATCAAATTGTATTTTTGCC
  ACATTTCTGTATTGGTGAAAAGCGAATCTGCAGAATAACTGTCAACGTTTTTCATTCTTGTTTTACAAAC
  CTATTTTTTAAAGTGAGAATAAGGTTGGTTTTTACCAAATATTTGTTCCTCCTTGACAAAAGTAGCTCTG
  TGTGGATAATATGATTTTATTACTACAGTTCCAGTCACTTGGTTATATTTATCTTAGCATGAGCCTTTCA
  CACCAAGATTTCTATATTTTGTAACATAGGTGAAATATTTAAAACATCAAAAACTGTAAATCTAGATTTT
  TAAAAAATCCAACTGCAATGTCTTTTCCTTTGATTGAGATGATTTGTGTAAACTCACCAGTCTTGCTTTG
  GAGTGAGCAGAAGAGAATGACTATTTTACGTGGAGCATCATTGTGTGACTGTTGACCTGGACAGTCCCAA
  GGGCTATGCAGATGGACTCCATTGGCACAGGGAGGTTTGACCTCTTCCCTGCTATTATCCCTCCTCCCAA
  CTGTACAGATTTGTTTGTTGTAGTTTATGTACTTCTTGATACTGTCAAAAAATTATCTGGAAATGGTTTT
  ATTTTGTGAAGTGAACAATACTTTGTAATTTATGATAGTGTAAATGGAAGGCAAAGCATCACATG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001039936.1
• Summary: 'This gene encodes a guanosine triphosphate (GTP)-metabolizing protein that contains a phorbol-ester/diacylglycerol (DAG)-type zinc finger, a Rho-GAP domain, and an SH2 domain. The encoded protein translocates from the cytosol to the Golgi apparatus membrane upon binding by diacylglycerol (DAG). Activity of this protein is important in cell proliferation and migration, and expression changes in this gene have been detected in cancers. A mutation in this gene has also been associated with schizophrenia in men. Alternative transcript splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, May 2014]'
• Locus ID: 1124