CACNA1A (NM_001127221) Human 3' UTR Clone

CAT#: SC215555

3`UTR clone of calcium channel voltage-dependent P/Q type alpha 1A subunit (CACNA1A) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CACNA1A
• Synonyms: APCA; BI; CACNL1A4; CAV2.1; EA2; EIEE42; FHM; HPCA; MHP; MHP1; SCA6
• ACCN: NM_001127221
• Insert Size: 1602 bp
• Sequence Data:
  Insert Sequence

  >SC215555 3'UTR clone of NM_001127221
  The sequence shown below is from the reference sequence of NM_001127221. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CGAGAGCACATGGCGCACCGGCAGTAGTTCCGTAAGTGGAAGCCCAGCCCCCTCAACATCTGGTACCAGC
  ACTCCGCGGCGGGGCCGCCGCCAGCTCCCCCAGACCCCCTCCACCCCCCGGCCACACGTGTCCTATTCCC
  CTGTGATCCGTAAGGCCGGCGGCTCGGGGCCCCCGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCA
  GCAGGCGGTGGCCAGGCCGGGCCGGGCGGCCACCAGCGGCCCTCGGAGGTACCCAGGCCCCACGGCCGAG
  CCTCTGGCCGGAGATCGGCCGCCCACGGGGGGCCACAGCAGCGGCCGCTCGCCCAGGATGGAGAGGCGGG
  TCCCAGGCCCGGCCCGGAGCGAGTCCCCCAGGGCCTGTCGACACGGCGGGGCCCGGTGGCCGGCATCTGG
  CCCGCACGTGTCCGAGGGGCCCCCGGGTCCCCGGCACCATGGCTACTACCGGGGCTCCGACTACGACGAG
  GCCGATGGCCCGGGCAGCGGGGGCGGCGAGGAGGCCATGGCCGGGGCCTACGACGCGCCACCCCCCGTAC
  GACACGCGTCCTCGGGCGCCACCGGGCGCTCGCCCAGGACTCCCCGGGCCTCGGGCCCGGCCTGCGCCTC
  GCCTTCTCGGCACGGCCGGCGACTCCCCAACGGCTACTACCCGGCGCACGGACTGGCCAGGCCCCGCGGG
  CCGGGCTCCAGGAAGGGCCTGCACGAACCCTACAGCGAGAGTGACGATGATTGGTGCTAAGCCCGGGCGA
  GGTGGCGCCCGCCCGGCCCCCCACGCACCCCACGCACACACCCCACCCGAGGAGCCGCGCAGAGGCCGCG
  GGGGCCCAGCACAGAGGGCCCGGGAGAGGGCCAGCCGGGAGACCCCAGACTCTGGAGAGGCCAGGGCTGG
  GCCACAAGGGTGTCCCGCAGAGACCCTCGGCCAAAAGAGACCCTCCTGGGCAGCCACGGCGCCCCCCAAC
  CAGCCCCGATCCCCCCACCCACGACAGGGGCTCTCGGGTGGGAGGCAGGGAGCAGACAAACCACACAGCC
  AAGGGATTTGAATTAACTCAGCCATTTTTGGAGAACTTTGGGGAACATGAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAACATTTTTAAAAGAAAAAACGGGGAGAAAAAAATAGCTTCTATTGATGAGTTTTATCATCTCA
  ATTGAATCTTTCCTTTCCCTGATGAAGACAGCTGGTGGCCGAGTGCGGCAAAGAAGCCAGAAGGAACCAG
  AATCCCAGTGCCCTACACCCACCACCAGACACACTCACACCCACACACGTTCTCAGACACACACAAGAGT
  GCTTGCCGGTTATACCAAACCCTACTATTACTGCCTGCAGAAATCAATTTAAAAAAATAATAATAACAAT
  AAACAATTTTAAAAAGGACAAAAAAATTAATGATTGAGAAAAGAGGCATTTTTTTCTGACATTTGGTCCT
  GCTTGAAACAACAAAAGAAGAAGAAAAACCCACCATCACCACCGATTCCTTTGCTTCTTTTTTCCTTTTT
  TCCTACCTTGTTTGAAAACCGTGGGCTTGGGACTGTGAATTATTGCATGACATTCAAAAAGA
  
  CGGACCGTTACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-RsrII
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001127221.1
• Summary: 'Voltage-dependent calcium channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, and gene expression. Calcium channels are multisubunit complexes composed of alpha-1, beta, alpha-2/delta, and gamma subunits. The channel activity is directed by the pore-forming alpha-1 subunit, whereas, the others act as auxiliary subunits regulating this activity. The distinctive properties of the calcium channel types are related primarily to the expression of a variety of alpha-1 isoforms, alpha-1A, B, C, D, E, and S. This gene encodes the alpha-1A subunit, which is predominantly expressed in neuronal tissue. Mutations in this gene are associated with 2 neurologic disorders, familial hemiplegic migraine and episodic ataxia 2. This gene also exhibits polymorphic variation due to (CAG)n-repeats. Multiple transcript variants encoding different isoforms have been found for this gene. In one set of transcript variants, the (CAG)n-repeats occur in the 3' UTR, and are not associated with any disease. But in another set of variants, an insertion extends the coding region to include the (CAG)n-repeats which encode a polyglutamine tract. Expansion of the (CAG)n-repeats from the normal 4-18 to 21-33 in the coding region is associated with spinocerebellar ataxia 6. [provided by RefSeq, Jul 2016]'
• Locus ID: 773