PREX1 (NM_020820) Human 3' UTR Clone

CAT#: SC215722

3`UTR clone of phosphatidylinositol-345-trisphosphate-dependent Rac exchange factor 1 (PREX1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PREX1
• Synonyms: P-REX1
• ACCN: NM_020820
• Insert Size: 1648
• Sequence Data:
  Insert Sequence

  >SC215722 3'UTR clone of NM_020820
  The sequence shown below is from the reference sequence of NM_020820. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TCTACCGCCTCTGCCAGCCGCCGGTGGATGGGGACCTCTGAACACCCAAATGCCCCACGCTGGGCCGCGG
  CCTCTGGAGCTGGGATTTGGGAGGACACAGCAGGCAGCGCTGGCCTTCTCCAGGGATGGCCCAAGGCTTC
  CGCAGCCGCCCGTTCCGGGACCTGCCCAGCGTCCTCCCTGCCTCCTTCCGGGACAAGCCTGGCCACCCTC
  GCTGTGATGACGAGCTGGCTGATTGGCCCTGGGCCGGCCCATTCTTCACACGCCTGCCAGAAGCTGGAGG
  GGTGCTGGAGACCCATAGAGCTGATGGGAGCAGCTGGTGCCTGGCCTTCGGCTCCTGCGTCCCCAGAACC
  CAAGGGAACGTCATGGAGGCCACATGGGGCCACCCGGCTCCCTCGGGATGGCTCCGCCTGCACTTTTGAA
  ACCCCGGTTTCCTTCAACGTCCACATTCCAGGTGACCACACGTGTCTCCTCCTCCTCATCTTAGCTTCCA
  GGTTCACCCTAACCCTGTACTAACCTGCTTGGTGGACTTGGAAAAGACTTGGCTCTGTCGGGAAAGGAGA
  GACGGGGCCTCCATCACGCCTGTTACCAGAGGATCCCCGAGAGCCACACCAGCTCTGGACATCACCGCCC
  CTGGAACTGGGGCCACCAGCCCTGGGCACGAGATTTGCTCTGACTTTATTTATATGGCATGAAATCTCTG
  GTTTATTTTGGGATTTTTTGTTGTTGGTGTTGTCAAAGTTTGTTTTTTCTAAAGTTGTGTGATTATATAT
  TTGACATTTTACATTTCAAAGAAAGGTATGTTGTCTAACAGGGGACCAACAGAAGGTAGTATTGACAACT
  GTTCCTGCTTCTACTAAAAAAAAAAGAGCACAAAAGAAAAACTAAATTATTGAAAAATTAAAAAATGTCA
  TTGTTTCCTGTTTGTTAATATTAGGGTTGTAAGGTGTCGTTTTGAGGTATCGACTGTGATTCCTTCCCCC
  ACCCTCCATTCTCCAGCGGTTGGCCGGTGTTAGAACTCGCTCTCTTTGAGTGACTGGCTACAAGGGCCTG
  AGAGGTGGCCAGCCAGGGTTGGAGCTGGAGGGGATGGAGCCCCACCTGAGGTGCCGTGTCACACGGGTTA
  GAGGGTCACTGGGAAACACCGGGCGGTGGCTTCTGTGATTTATTTTCTTGATGGTAACTTCTCAGAGCAG
  GGCGATTGGGACATCACCAGCCAGAGCACAGGAAGCCACCCTGCCTGCTGGGGAGGAGGGACCCACACAA
  GCCCCCTCGGCAGTTTGTCCCCCCAGCTTCGGTATGCCTTCAGGGAAAGGTCACAGCTGGGGAGGAAGCG
  GGGGGACGCCTGTCACCCCTGGCAGGTGGTGAGTTCAGGTGGGGGCTCCCTGCTGCCCCCAGGCCTGGGA
  GCTTGAAGCCCTCCCGGCATCTGGCATCCGAGCCTCCCGCCCTCCAGGGTGCGCTTCCCTCTCTTGCCGC
  AGCATACACGAGGGCAGGCAGTGGCCTTGTCACTGTATCTTGCATCAGAGACAAAGGAGGACCCGCTTTA
  GCCCTGCTGCGGGAAATGGGGGAGGGCCCAGGGCCAGCGCATTGTGCACTGGTTTACTTTAAAATGTACA
  GATTCTTCTCGTTAAATTCTTGATAGATTTTTTATTAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_020820.3
• Summary: The protein encoded by this gene acts as a guanine nucleotide exchange factor for the RHO family of small GTP-binding proteins (RACs). It has been shown to bind to and activate RAC1 by exchanging bound GDP for free GTP. The encoded protein, which is found mainly in the cytoplasm, is activated by phosphatidylinositol-3,4,5-trisphosphate and the beta-gamma subunits of heterotrimeric G proteins. [provided by RefSeq, Jul 2008]
• Locus ID: 57580