ROCK1 (NM_005406) Human 3' UTR Clone

CAT#: SC215786

3`UTR clone of Rho-associated coiled-coil containing protein kinase 1 (ROCK1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ROCK1
• Synonyms: P160ROCK; ROCK-I
• ACCN: NM_005406
• Insert Size: 1651 bp
• Sequence Data:
  Insert Sequence

  >SC215786 3'UTR clone of NM_005406
  The sequence shown below is from the reference sequence of NM_005406. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGTGGTCAAAAATACATCTGGAAAAACTAGTTAACCATGTGACTGAGTGCCCTGTGGAATCGTGTGGGAT
  GCTACCTGATAAACCAGGCTTCTTTAACCATGCAGAGCAGACAGGCTGTTTCTTTGACACAAATATCACA
  GGCTTCAGGGTTAAGATTGCTGTTTTTCTGTCCTTGCTTTGGCACAACACACTGAGGGTTTTTTTTATTG
  CGGGTTTGCCTACAGGTAGATTAGATTAATTATTACTATGTAATGCAAGTACAGTTGGGGGAAAGCTTAG
  GTAGATATATTTTTTTTAAAAGGTGCTGCCTTTTTGGATTTATAAGAAAATGCCTGTCAGTCGTGATAGA
  ACAGAGTTTTCCTCATATGAGTAAGAGGAAGGGACTTTCACTTTCAAGTGGAACAGCCATCACTATCAAG
  ATCAGCTCATGGAAGGAGTAAAGAAAATATCTCAAAATGAGACAAACTGAAGTTTTGTTTTTTTTTTAAT
  GACTTAAGTTTTTGTGCTCTTGCAAGACTATACAAAACTATTTTAAGAAAGCAGTGATATCACTTGAACT
  TCAGTGCCCTCACTGTAGAATTTAAAAGCCTTACTGTTGATTGCCCATGTTGGACTTGATGGAGAAATTA
  AATATCTTTCATTATGCTTTACAAAATACTGTATATGTTTCAGCAAGTTTGGGGAATGGGAGAGGACAAA
  AAAAAGTTACATTTAATCTATGCATTTTTGCCAAGCCATATTGAGTTATTTTACTACTAGAGACATTAGG
  AAACTAACTGTACAAAAGAACCAAGTTTAAAAGCATTTTGTGGGGTACATCATTTCTATAATTGTATAAT
  GTATTTCTTTGTGGTTTTAAATGATAAAGACATTAAGTTAACAAACATATAAGAAATGTATGCACTGTTT
  GAAATGTAAATTATTCTTAGAACACTTTCAATGGGGGTTGCATTGTCCTTTTAGTGCCTTAATTTGAGAT
  AATTATTTTACTGCCATGAGTAAGTATAGAAATTTCAAAAAATGTATTTTCAAAAAATTATGTGTGTCAG
  TGAGTTTTTCATTGATAATTGGTTTAATTTAAAATATTTAGAGGTTTGTTGGACTTTCATAAATTGAGTA
  CAATCTTTGCATCAAACTACCTGCTACAATAATGACTTTATAAAACTGCAAAAAATGTAGAAGGTTGCAC
  CAACATAAAAAGGAAATATGGCAATACATCCATGATGTTTTCCAGTTAACATAGGAATTACCAGATAAAT
  ACTGTTAAACTCTTGTCCAGTAACAAGAGTTGATTCATATGGACAGTATGATTTATTGTTTATTTTTTTA
  ACCAAATACCTCCTCAGTAATTTATAATGGCTTTGCAGTAATGTGTATCAGATAAGAAGCACTGGAAAAC
  CGATCGTCTCTAGGATGATATGCATGTTTCAAGTGGTATTGAAAGCCGCACTGATGGATATGTAATAATA
  AACATATCTGTTATTAATATACTAATGACTCTGTGCTCATTTAATGAGAAATAAAAGTAATTTATGGATG
  GGTATCTTTAATTTTTACTGCAATGTGTTTTCTCATGGCTGAAATGAATGGAAAACATACTTCAAATTAG
  TCTCTGATTGTATATAAATGTTTGTGAAATTCCATGGTTAG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005406.2
• Summary: 'This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18. [provided by RefSeq, Aug 2015]'
• Locus ID: 6093