Adducin 2 (ADD2) (NM_017484) Human 3' UTR Clone

CAT#: SC215819

3`UTR clone of adducin 2 (beta) (ADD2) transcript variant beta-3b for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ADD2
• Synonyms: ADDB; Adducin-2 (beta); adducin 2; adducin 2 (beta); beta adducin
• ACCN: NM_017484
• Insert Size: 1634 bp
• Sequence Data:
  Insert Sequence

  >SC215819 3'UTR clone of NM_017484
  The sequence shown below is from the reference sequence of NM_017484. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGGTCTTCTGTGCCTGGGGCTGGGTTGCAGGACCCCTGAGTCCTCATACCCACCTTCCCCTGTGATCTTG
  GGTGCCGGTGCTCTTGCTGCAAAATGGAGGCCAACCTCACAGGGGTGCTGTGAGGACCAGGGAGGACAAT
  GTCTGATGGCCCCTGTGGGCACTGGGCTGGAGAACAGCAGCCCAGCCTGCCTGCAGGAGTGATGCGGCCG
  GCTTCCGTACCTCCTGCCATGGAACTCCACTCTGTCCTCCCTTACTACCCCGTGAAGTGGGGACTCATAG
  GCTTGGGGCTGGAGACTCCCAGGTGCCCCAGGTGTGCCCAGCCCTGAGAGTCTCTGACCTCTGAGCCTGG
  TATGACATCATCCAACCAGAGCTCTGGAAAGATGATCATGTACCCATAGAGAAGGGGTATATAGACAGGA
  CCCATGCTCCTACAGGATGGGAAGGGACAATATTACCCACACAAACCCACATTGAGTTAGAAAGTATTGT
  AATTGTTTTTGTGCTTGAGATAGAACAGTGTTTTGTCTAGTGCTTAGCAAAAGGGGTGAAGTTCAGAATT
  CTGGCATGTGTTTGAAATCACTGCTGCTCCTTGCAAGGTAGCAATGGGCATGACATTTTCCTTAGCAGTA
  AAAATTGGGGAAATACAGGAAATGTTCCTCTCTGCAGGGCAAATTGTGATGATGCAATGATTTCAGGTCA
  AGACTGCAGCCCACAGGGTGGGTGCCCATTGGCAGGAGAAGTGTGAAAGTTGTATCATCACACTTACCTC
  CCCCCAACCCCAACTCAGCCAAGATGGGGAACACTGAATATCCCGCAGGGTCAAGTATAGAAATAGCCAT
  GTTCTGGCGGGAGGTGAGAGTTCATTACAATGAGCAACACTGTTAAAAAAACAAACAAAAATCTCCCCTG
  CCATTTATACCAAGTGCTCTTCTCGGAGTCCCTCTTCTTGGGTTTTTGGTCTTGGATTCTCGGTTGACTC
  CATGATTCCCTACATTAGGCCTACTGTGGCCTCCCTTTTGGAAGGGGCCCAGGGGCAGAAGACCTGGCGG
  GCCAGGGCAGGGAGGACTGGCAGCCTCCAGGCTCACCTCCCTTTGGCTTCACTCTCTTTGCTCCTCAGCT
  TCAGAGCACTGCATCCTCAAAGGCCAAGATGAGGATGCTGAATTAGGAAAAAAGGCTGTGGAGTCTGAAT
  TTTAGTTCCTATCTTGGGCTAGAGGGGTCAAAGAATTCCTGAAGCTTATGTAAGGGCCGGCTGGTCAGCT
  CCCTAGAACCACGGGCTCCACAGAGGTGGCAAAACCCAGTGATTTTGCCAGTATCCCTGCAGGGACCTGC
  GGTACAGGCAGGTTGAAGATCTGAGACTCAGGAACCTTGGCCCCTTTGTCACTTACAACTGTGTGTGAAA
  CAGGACTAACAGTTTGGACTGAGAGCACTGAAGCTGCTGTGTGTTGTGCATATAACTTAATGCTGTGTGT
  TGTGGCTTGCACTGCTGTGGGGGCGGGGAGGGCAGTGGTGGGGAATGGCTGGCATGAAGCTTGCTTGAGC
  CAATGCTATGCTTGCTGGGCTTTGGCTGACTGGCTTTGGGCCACCAACTGTATTTGCATAGTGATGGCTT
  GGAAGTGTCCATAGAAGGGATTGC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_017484.2
• Summary: 'Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jun 2010]'
• Locus ID: 119