EDG2 (LPAR1) (NM_057159) Human 3' UTR Clone

CAT#: SC215867

3`UTR clone of lysophosphatidic acid receptor 1 (LPAR1) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: LPAR1
• Synonyms: edg-2; EDG2; Gpcr26; GPR26; LPA1; Mrec1.3; rec.1.3; vzg-1; VZG1
• ACCN: NM_057159
• Insert Size: 1676 bp
• Sequence Data:
  Insert Sequence

  >SC215867 3'UTR clone of NM_057159
  The sequence shown below is from the reference sequence of NM_057159. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CACCATCTTGGCTGGAGTTCACAGCAATGACCACTCTGTGGTTTAGAACGGAAACTGAGATGAGGAACCA
  GCCGTCCTCTCTTGGAGGATAAACAGCCTCCCCCTACCCAATTGCCAGGGCAAGGTGGGGTGTGAGAGAG
  GAGAAAAGTCAACTCATGTACTTAAACACTAACCAATGACAGTATTTGTTCCTGGACCCCACAAGACTTG
  ATATATATTGAAAATTAGCTTATGTGACAACCCTCATCTTGATCCCCATCCCTTCTGAAAGTAGGAAGTT
  GGAGCTCTTGCAATGGAATTCAAGAACAGACTCTGGAGTGTCCATTTAGACTACACTAACTAGACTTTTA
  AAAGATTTTGTGTGGTTTGGTGCAAGTCAGAATAAATTCTGGCTAGTTGAATCCACAACTTCATTTATAT
  ACAGGCTTCCCTTTTTTATTTTTAAAGGATACGTTTCACTTAATAAACACGTTTATGCCTATCAGCATGT
  TTGTGATGGATGAGACTATGGACTGCTTTTAAACTACCATAATTCCATTTTTTCCCTTACATAGGAAAAC
  TGTAAGTTGGAATTATCTTTTGTTTAGAAAGCATGCATGTAATGTATGTATGCAGTATGCCTTACTTAAA
  AAGATTAAAAGGATACTAATGTTAAATCTTCTAGGAAATAGAACCTAGACTTCAAAGCCAGTATTTGTTT
  AGGTCATGAAGCAAACAATGCTCTAATCACAATATTAACTGTTTAATTAAAATGTTGTAACAAGTATAAA
  ACAGGGAATGTAAGTTTATTACCAAAGTGATATGTATTCCAAAAAAGTCATAGAAGATGAAGCACTATAA
  TATTGTTCCCATATATTTAAAATACCCAAGTACATTCTAATTACCAGTATATCAGAGGAAAATTTTCGTA
  GTCTTTGTAAAATAATATACTCATCATAGAAAACTTGAAAAATGCAGAAATGTATAAAAAAGCAAAAATG
  ATTACTGATAATATCACAACCCAGAAGTAACCACCTTTAAAAAGCAACCCCCATGTATGCCTATATGTGT
  ATTGTATACTTTTTTTACATAATTGGAGTCATACTGTAAACAGTTTTATAAGTAGATCTTTTTCATTGCA
  AAATTGCCACATTTTCTTATGGCATTAAAAATTTTACAAAAACATAATTTTAATGGCTATATTATATTCC
  ATTTAATGGATGCAACTCAGTTTATTTAACCATTCCCATGTTGTTAACTATTTAGGTTGTTTCTAATTTT
  CATTATTATAAAGTTGCAGAAATTTGGTGTACATAAAACTGTCTCCATATAATTGATTATTAGGATATAT
  TCCCATGAAGGATTCTTTTTTTAAAAAAATGTGAAATGTCATCTTGTACTTACACCTTTCATGAAAAGGG
  ATTTCCTGCTTTTGTACTGCATGGGTGGCAGTTGTGAGGAAAAGCCAGTCAAATGACCTTTTTACAAAAG
  AAATGCAGTGGTCACTTCAGTTGAGAGTGACTTTTTAATACAACAAGATCAACTAGAAGAATTCAACTGT
  CTCAAGAATCAAGGTACCCCAATATATCTCGCAATTCCAAACTTTGTTTGAGGGACTCGTTATCCAGCTC
  TTGGTAGCCACACCTGCAATGTAAAATGGAAGAAAATGCAAAGAAACCAAATGTGCCGAGTGAATA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_057159.2
• Summary: 'The integral membrane protein encoded by this gene is a lysophosphatidic acid (LPA) receptor from a group known as EDG receptors. These receptors are members of the G protein-coupled receptor superfamily. Utilized by LPA for cell signaling, EDG receptors mediate diverse biologic functions, including proliferation, platelet aggregation, smooth muscle contraction, inhibition of neuroblastoma cell differentiation, chemotaxis, and tumor cell invasion. Two transcript variants encoding the same protein have been identified for this gene [provided by RefSeq, Jul 2008]'
• Locus ID: 1902