GLRB (NM_001166061) Human 3' UTR Clone

CAT#: SC215897

3`UTR clone of glycine receptor beta (GLRB) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: GLRB
• Synonyms: HKPX2
• ACCN: NM_001166061
• Insert Size: 1658 bp
• Sequence Data:
  Insert Sequence

  >SC215897 3'UTR clone of NM_001166061
  The sequence shown below is from the reference sequence of NM_001166061. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ACGCGAGTGCTGCCAGAGTGCCCCTGGGTTGGTGAGACCAGATGCAAAAAAGTTTGTACTTCTAAGTCTG
  ATCTGAGATCTAATGACTTCAGCATTGTTGGAAGCTTACCAAGAGATTTTGAACTATCCAATTATGACTG
  CTATGGAAAACCCATTGAAGTTAACAACGGACTTGGGAAATCTCAGGCTAAGAACAACAAGAAGCCTCCC
  CCTGCGAAACCTGTTATTCCAACAGCAGCAAAGCGAATTGATCTTTATGCAAGAGCATTGTTTCCTTTCT
  GCTTCTTGTTCTTCAATGTTATATATTGGTCTATATATTTATGATAAATCTTTTCCATTTGTACAAAATA
  AAATTCCATTTCATTGTGACCTACTCCTTTCATAAATGCCAATCTGTGAGAACTTTTGAATTTTCATAGC
  AACATTGCATTTTGGATGCCATTTGATTGTAATAAAACTGTGGCACCTTAATTTTGAATGGCAGCATGAT
  CATGTAATATCTGTGCTCTAATAACGATGTATATATGTATAGTGAACATATTGCTTAGTAACAAATGAAG
  GACAAGCATACTACATAATATAATCCATACAATTCTCTTCAGTTAGTGTAAACTGCAAATACTACAGATA
  ATTCTGATAATAAAATGATATGCACGCTGAATCCTGCTATGGTCACCATTCTAATGTATGTAGTATTTCA
  AATTTCCTTCCTTGTAACTTTCAAAGAAAGCCATCTTATTCTTGTAAAATTTTAGATGGTATTATCACAG
  ATTTAAAAAGGTTGTATTACATATTGTTTAAACTTTGTAAGTAGAAATATATCTGTTATAATTATACAGG
  CTCTGTGGAGAAATAAAGTTCAAAATATATTAATTTGTAAAATCAGCTCGTTTTAAAGTGTGCTTGTGTT
  GTCAAAAATATCAGATAGTAATACACAGTGAGCATTTTTAAACAAAGGGAAACCTATATTTATGTAACTG
  TATACTGAATTCTGACAAAATAAAAAAAGATACCTTATTGACGAAATATTTAGGATAAACAAAATTCTAT
  TTAATCCACCTTAAAACCTAAATGTATTTTCATGGATTTCATTTGTTGGTACATATTACACAAAACATTG
  TGCCTTAAAATGAGTCATACATCTTTTAAATTGGAATGCAGTAATAGATATGTGATTTTACATCATTTTT
  AAGAAACCAAGGGGAAGTAATAAGTTGAAAAAGAAATCCATAACTATTAAAAGATTTTAACTTTTTTATT
  TTATTAAAATGCTTGCATATTTTAAGTAAAATTAAAAATGTTTACTGAATTTATTTTTTTATTTGAATAT
  TTTGGGATTAGTTACAAAATATTTAGAGATTAAATAAATAATAGCTTTGTTTGTGTTAGACTTCACTTCT
  ACTTGTATTTTCTTTCTTGTTAAAAGTCTAGACAACTGAGATTTACTTACTTTGAACTTGTTCCAATCCA
  AAAGTAAGCACTAAGTCTCATTTTATGAGACCACCATTTCTTAATATCACATCCAGTGCACCTTTGTCTT
  TCTGCCATATCTGAAATAAAACTATCAGTAATTCACATAGATAAACATAAGACTAAAGAAGTATATTTAC
  ATTATCTGGAGAGTTTTGTTGCAGCTACGAGTTTGTATGGCAAATTCA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001166061.1
• Summary: 'This gene encodes the beta subunit of the glycine receptor, which is a pentamer composed of alpha and beta subunits. The receptor functions as a neurotransmitter-gated ion channel, which produces hyperpolarization via increased chloride conductance due to the binding of glycine to the receptor. Mutations in this gene cause startle disease, also known as hereditary hyperekplexia or congenital stiff-person syndrome, a disease characterized by muscular rigidity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]'
• Locus ID: 2743