B7-2 (CD86) (NM_006889) Human 3' UTR Clone

CAT#: SC215945

3`UTR clone of CD86 molecule (CD86) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CD86
• Synonyms: B7-2; B7.2; B70; CD28LG2; LAB72
• ACCN: NM_006889
• Insert Size: 1619 bp
• Sequence Data:
  Insert Sequence

  >SC215945 3'UTR clone of NM_006889
  The sequence shown below is from the reference sequence of NM_006889. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CGAAGACATCTTCATGCGACAAAAGTGATACATGTTTTTAATTAAAGAGTAAAGCCCATACAAGTATTCA
  TTTTTTCTACCCTTTCCTTTGTAAGTTCCTGGGCAACCTTTTTGATTTCTTCCAGAAGGCAAAAAGACAT
  TACCATGAGTAATAAGGGGGCTCCAGGACTCCCTCTAAGTGGAATAGCCTCCCTGTAACTCCAGCTCTGC
  TCCGTATGCCAAGAGGAGACTTTAATTCTCTTACTGCTTCTTTTCACTTCAGAGCACACTTATGGGCCAA
  GCCCAGCTTAATGGCTCATGACCTGGAAATAAAATTTAGGACCAATACCTCCTCCAGATCAGATTCTTCT
  CTTAATTTCATAGATTGTGTTTTTTTTTTAAATAGACCTCTCAATTTCTGGAAAACTGCCTTTTATCTGC
  CCAGAATTCTAAGCTGGTGCCCCACTGAATTTTGTGTGTACCTGTGACTAAACAACTACCTCCTCAGTCT
  GGGTGGGACTTATGTATTTATGACCTTATAGTGTTAATATCTTGAAACATAGAGATCTATGTACTGTAAT
  AGTGTGATTACTATGCTCTAGAGAAAAGTCTACCCCTGCTAAGGAGTTCTCATCCCTCTGTCAGGGTCAG
  TAAGGAAAACGGTGGCCTAGGGTACAGGCAACAATGAGCAGACCAACCTAAATTTGGGGAAATTAGGAGA
  GGCAGAGATAGAACCTGGAGCCACTTCTATCTGGGCTGTTGCTAATATTGAGGAGGCTTGCCCCACCCAA
  CAAGCCATAGTGGAGAGAACTGAATAAACAGGAAAATGCCAGAGCTTGTGAACCCTGTTTCTCTTGAAGA
  ACTGACTAGTGAGATGGCCTGGGGAAGCTGTGAAAGAACCAAAAGAGATCACAATACTCAAAAGAGAGAG
  AGAGAGAAAAAAGAGAGATCTTGATCCACAGAAATACATGAAATGTCTGGTCTGTCCACCCCATCAACAA
  GTCTTGAAACAAGCAACAGATGGATAGTCTGTCCAAATGGACATAAGACAGACAGCAGTTTCCCTGGTGG
  TCAGGGAGGGGTTTTGGTGATACCCAAGTTATTGGGATGTCATCTTCCTGGAAGCAGAGCTGGGGAGGGA
  GAGCCATCACCTTGATAATGGGATGAATGGAAGGAGGCTTAGGACTTTCCACTCCTGGCTGAGAGAGGAA
  GAGCTGCAACGGAATTAGGAAGACCAAGACACAGATCACCCGGGGCTTACTTAGCCTACAGATGTCCTAC
  GGGAACGTGGGCTGGCCCAGCATAGGGCTAGCAAATTTGAGTTGGATGATTGTTTTTGCTCAAGGCAACC
  AGAGGAAACTTGCATACAGAGACAGATATACTGGGAGAAATGACTTTGAAAACCTGGCTCTAAGGTGGGA
  TCACTAAGGGATGGGGCAGTCTCTGCCCAAACATAAAGAGAACTCTGGGGAGCCTGAGCCACAAAAATGT
  TCCTTTATTTTATGTAAACCCTCAAGGGTTATAGACTGCCATGCTAGACAAGCTTGTCCATGTAATATTC
  CCATGTTTTTACCCTGCCCCTGCCTTGATTAGACTCCTAGCACCTGGCTAGTTTCTAACATGTTTTGTGC
  AGCACAGTT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_006889.3
• Summary: 'This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]'
• Locus ID: 942