INSIG2 (NM_016133) Human 3' UTR Clone

CAT#: SC216121

3`UTR clone of insulin induced gene 2 (INSIG2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: INSIG2
• Synonyms: MGC26273
• ACCN: NM_016133
• Insert Size: 1707
• Sequence Data:
  Insert Sequence

  >SC216121 3'UTR clone of NM_016133
  The sequence shown below is from the reference sequence of NM_016133. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CGCAGAAAAATCTCATCAGGAATGAAGAAGGCAAAAAATATCTTTTGTACAGAAAAGCAAGATGAAAAGG
  ATGTGAAATGGTAGATATACCAACAAAACTTCAGACTGTAAAATTGCCAGGATGCAGTTTTCCCCTTGAT
  TGGCGTGTGTGTATATATGGATAAATATATATATACACACACACATATTACTGCAATCTGTGATTGCTTC
  ATCTGTAAATCAGTTGTAAACCTTTACATATTTGACTTAAATAACTGTAAGATATATATGTACTACATTA
  AAAAGTGTTGATTAATAGATGAAATTTTTAAATTAATTTTTTAAAACATGCCATACATTGTATCACAATG
  TTAATGTGCCAAGATATTGTTCCTGTCATGCAGAGTATAAGAATGCTTTGAACAATTTGTAGACTTAGTG
  AAATAAAATAAGAGGAAAGCCAAAAACAAACAAACAAAAAGCATATGGGGAGCTGGTATTTTCTCTTTAG
  CTTACTGTTGTGCCTTTTTATTTTTCTAATCACAGCAGTATGAGTTATGAGTGCCCTAATTTGTGGTTAG
  TTTCTAATTTAATGTTGTTTCATAGAGTTTGGAGTGTTTTGATACAGGGTGAAAATGAACTTCTGGTTTC
  AAACCTGCGTTACTGGAGACAGCCCAAAGAGTAATTTTCTGTTTTGACAGGTTTTACTGGAAGTATATGT
  GATGAGCAGAAGAGGTTATCAGCATTAAATTGTTTTGGTTCTAAATTTGGAACAGTATATATAATTAAAA
  GTAAGGAACATTAGAGGATTTAATTAGAATAAATACATGTTTTGGAAATACAGTGACCTCTTGCAGTGTC
  ACAAAAGTGCAAAGTGATATTAGCTGTCATCTGCAATACAGAATCTCATTGCTTTTGCACATGGAGCATA
  TAGGAAACTCCAAACAGATCACAATGAGGTTTCTAAATCTGTTGGGTTCTGTCTTCTATTGGGTTCTGTG
  AAGCAAACCACTGTAGCTTTAGCTGGGTTCAGTCATATGACTCGTTGGTGGAATGCCTAGGTTTTTCATC
  TTACATGCAGTCTTGGGGGTGGATGAATACATAATTTCTTATGTATTCGTGTATCCATTAGTGAATAGTT
  CAAGTCTGTTTAAGAGTGTATTGAGATGGCATTCTCTGCATGTTAAAGATCTTAATGGCAACCAGCACCT
  CTTAAGTATGGTTTAAACATATTCTTAGCTAATTTTTTCCATTAGTTTTTGAAATTGGTGGCAGTTGTCT
  GATCCACAAGGGCAAGATCTTCTGAGTACTCTGGGGTGTGAGTATGTGTGCACACGTGTGTGTTGGAGTG
  AGTGAGAGAATGTGTCTGTGCATGTGGCCATGCTTTCCTAGAATGTCAAGTAGATATTTTTACACTTTGA
  GTTTTAAAGCAATTACTATCAGACTGAGATCTTGTATGCCAAACTTTAATCTGCTTTTATGTTTTCAGGC
  TGAAGGTGTGAAAATCCTAAGAGGATTTCATATTGAATATGTGTACACAATCTTAACTATCGTGGTGGAA
  AACATACTACTATAATTTATTATTATATCTTCCAGATAATGTTATTCATTTAGAACAAATAAGGTATATT
  TTTTAGAATCAACTTTGTAAGCACTATAAAATCTTTAATAAGTTATAAGGTCTATGATGTGTTTACTTTA
  AAAATTGCTGTTAAAAGCAACACGTAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_016133.2
• Summary: The protein encoded by this gene is highly similar to the protein product encoded by gene INSIG1. Both INSIG1 protein and this protein are endoplasmic reticulum proteins that block the processing of sterol regulatory element binding proteins (SREBPs) by binding to SREBP cleavage-activating protein (SCAP), and thus prevent SCAP from escorting SREBPs to the Golgi. [provided by RefSeq, Jul 2008]
• Locus ID: 51141