GJA1 (NM_000165) Human 3' UTR Clone

CAT#: SC216210

3`UTR clone of gap junction protein alpha 1 43kDa (GJA1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: GJA1
• Synonyms: AVSD3; CMDR; CX43; EKVP; EKVP3; GJAL; HLHS1; HSS; ODDD; PPKCA
• ACCN: NM_000165
• Insert Size: 1726
• Sequence Data:
  Insert Sequence

  >SC216210 3'UTR clone of NM_000165
  The sequence shown below is from the reference sequence of NM_000165. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCCTGATGACCTGGAGATCTAGATACAGGCTTGAAAGCATCAAGATTCCACTCAATTGTGGAGAAGAAAA
  AAGGTGCTGTAGAAAGTGCACCAGGTGTTAATTTTGATCCGGTGGAGGTGGTACTCAACAGCCTTATTCA
  TGAGGCTTAGAAAACACAAAGACATTAGAATACCTAGGTTCACTGGGGGTGTATGGGGTAGATGGGTGGA
  GAGGGAGGGGATAAGAGAGGTGCATGTTGGTATTTAAAGTAGTGGATTCAAAGAACTTAGATTATAAATA
  AGAGTTCCATTAGGTGATACATAGATAAGGGCTTTTTCTCCCCGCAAACACCCCTAAGAATGGTTCTGTG
  TATGTGAATGAGCGGGTGGTAATTGTGGCTAAATATTTTTGTTTTACCAAGAAACTGAAATAATTCTGGC
  CAGGAATAAATACTTCCTGAACATCTTAGGTCTTTTCAACAAGAAAAAGACAGAGGATTGTCCTTAAGTC
  CCTGCTAAAACATTCCATTGTTAAAATTTGCACTTTGAAGGTAAGCTTTCTAGGCCTGACCCTCCAGGTG
  TCAATGGACTTGTGCTACTATATTTTTTTATTCTTGGTATCAGTTTAAAATTCAGACAAGGCCCACAGAA
  TAAGATTTTCCATGCATTTGCAAATACGTATATTCTTTTTCCATCCACTTGCACAATATCATTACCATCA
  CTTTTTCATCATTCCTCAGCTACTACTCACATTCATTTAATGGTTTCTGTAAACATTTTTAAGACAGTTG
  GGATGTCACTTAACATTTTTTTTTTGAGCTAAAGTCAGGGAATCAAGCCATGCTTAATATTTAACAATCA
  CTTATATGTGTGTCGAAGAGTTTGTTTTGTTTGTCATGTATTGGTACAAGCAGATACAGTATAAACTCAC
  AAACACAGATTTGAAAATAATGCACATATGGTGTTCAAATTTGAACCTTTCTCATGGATTTTTGTGGTGT
  GGGCCAATATGGTGTTTACATTATATAATTCCTGCTGTGGCAAGTAAAGCACACTTTTTTTTTCTCCTAA
  AATGTTTTTCCCTGTGTATCCTATTATGGATACTGGTTTTGTTAATTATGATTCTTTATTTTCTCTCCTT
  TTTTTAGGATATAGCAGTAATGCTATTACTGAAATGAATTTCCTTTTTCTGAAATGTAATCATTGATGCT
  TGAATGATAGAATTTTAGTACTGTAAACAGGCTTTAGTCATTAATGTGAGAGACTTAGAAAAAATGCTTA
  GAGTGGACTATTAAATGTGCCTAAATGAATTTTGCAGTAACTGGTATTCTTGGGTTTTCCTACTTAATAC
  ACAGTAATTCAGAACTTGTATTCTATTATGAGTTTAGCAGTCTTTTGGAGTGACCAGCAACTTTGATGTT
  TGCACTAAGATTTTATTTGGAATGCAAGAGAGGTTGAAAGAGGATTCAGTAGTACACATACAACTAATTT
  ATTTGAACTATATGTTGAAGACATCTACCAGTTTCTCCAAATGCCTTTTTTAAAACTCATCACAGAAGAT
  TGGTGAAAATGCTGAGTATGACACTTTTCTTCTTGCATGCATGTCAGCTACATAAACAGTTTTGTACAAT
  GAAAATTACTAATTTGTTTGACATTCCATGTTAAACTACGGTCATGTTCAGCTTCATTGCATGTAATGTA
  GACCTAGTCCATCAGATCATGTGTTCTGGAGAGTGTTCTTTATTCA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_000165.3
• Summary: This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia, autosomal recessive craniometaphyseal dysplasia and heart malformations. [provided by RefSeq, May 2014]
• Locus ID: 2697