CACNB1 (NM_000723) Human 3' UTR Clone

CAT#: SC216235

3`UTR clone of calcium channel voltage-dependent beta 1 subunit (CACNB1) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CACNB1
• Synonyms: CAB1; CACNLB1; CCHLB1
• ACCN: NM_000723
• Insert Size: 1733 bp
• Sequence Data:
  Insert Sequence

  >SC216235 3'UTR clone of NM_000723
  The sequence shown below is from the reference sequence of NM_000723. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GAGGCGTCTACATTCGCTGAGAGGCAGGGGCCACACGGCGGGAGGAAGGGCTCTGAGCCCAGGGGAGGGG
  AGGGAGCGAGGGGCTCACACCTGACATGTATTCGCCTCCAGGGGGCGCTGTCTCCCTCCTTTCAGATGCC
  TTTGCTCAAAGCTTGGGGTTTCTTTGGTGTTACCATCCCAGCTCCCGGGAGGCCCTTAAGCCCCAGCTGT
  CGGTTTTTACCTGCCTGTTGTGGATGGATGGGGGATACCCACCTTTCTGAAGTGTCCCCTTTCTCCCATC
  TTAAGGGGCTCTCCTCCCTCACCCTCCTAGAGAAAAGGTGCACTTCCTTAACTCTTTCTACTCGGGGCCC
  TAAGTGACGGTCCTAAGTGGGATGGCTCTCCTTCTCCCAAGCTGCAGTACTGGGGAAGGGCTGGGCGCTT
  TTCCTGGAAAGGGAGGCCACAGATTCTTTCCCATGGGGGCTCTCTTCCCCAGACCCCAGATCCAAGGTCC
  CTCACCCTGCCTGCCCCTTCCTCCCAGCTTCCTGGCAGCATCGTCTGGTCGGTGAAAGCCATAGCATGGA
  CACCCCATGGGGAGCTTGTCTTGGGGAGGGTTCTGGGTGGAAGCTGGCAGGCATACAGCACCCTCTACCC
  TCCGTGGCCATGGCAACGTCCAGGGCCCAGAACCCTGAGGAGTGAGCGGCCGAGACGCTGCTCCCCACCC
  CCCACCTCCATGCCTCAGCCTTTGCCTACCCCAGGAATGAGCTTGGCCTCCAACATCCCTTGCCTGCTGC
  CATTAGTAGAGGGGGCCCCTCTGCATCTGAGCCCCCCATCCCTGTGCCACCTGGGTGTGGAGCCCATGGA
  ACACTCTGGTCCGCCTCATTTTAAACCAAAAAACTGCTCCTTCACCCTCACCCTGAGGCCCCAGGGGAGA
  GGACCCGTGGGATGGTGCCCAGGGGTTGCCTTGGGACCTCGGATCTCCTCTGGGGGGCTTGGCTGCTGCT
  GTTGCTGCTCTGTATTTGCCTCTCGTGATTCTGTTTGTTACCCATGTTCACTTCCCCCAGGAGAGGCCTT
  GGTACCCCCTCTCCCCTGGGGCATCCCTTTGCCTTGGCATCCCTGTAGCCCAGCAACCCTGCCCCTCCCC
  AGCATCCCAGCTGGGCCAGAGAGAGCCGAGTGTGCCAACAAGGACTGGGGCCTGCCCGGCTGCCCGCCTC
  AGGGATGGGCACCTCATGCCTGTCTCGCCACCTCCTGTGCCAATGTCCCACCCTCCACCTGGGGGTGGGG
  TGCAGCTTCCACTTACTGATTAGAAGACACCACTGCCCTCCCTTCCCCCCTCCCTGTCTGGTGTCCTGTG
  CCCCCATCTGTCTGTCTATATTTGTCTGTACTCCCCTAGGAGAAGTATTTTGCCATATATAAAACCACTG
  TCCTGTCCTTTGTGGCTGCCTCCCAAGCCTGCTTCTTTGTCCTCGCCACATAGTCGTCAGCGTAGGCACC
  TGGGAGCTGCTGATATGCACGGGGAGTTGAAAGGGTGGGTGCCTGAAGATGTTGTGCCCTGAGTCATTGA
  CTCAAAAGAAAAGATGATCCTTTGATTTTGGCCCTCTGATGTATTGTGCCCAAGCCAGGAGCTGCTTGGG
  CAGTCCCAGCTCCACACTGGCCCTGAGCCCCTTCACTTACCTGTCTCTCCACAAGTAGAGCCAAAGGCAA
  TGGGAAGCTCAATGTTGCTCAGTGGGTGAGATCCAGACCCACTGGTGCAATGT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000723.3
• Summary: 'The protein encoded by this gene belongs to the calcium channel beta subunit family. It plays an important role in the calcium channel by modulating G protein inhibition, increasing peak calcium current, controlling the alpha-1 subunit membrane targeting and shifting the voltage dependence of activation and inactivation. Alternative splicing occurs at this locus and three transcript variants encoding three distinct isoforms have been identified. [provided by RefSeq, Jul 2008]'
• Locus ID: 782