ACSL3 (NM_004457) Human 3' UTR Clone

CAT#: SC216323

3`UTR clone of acyl-CoA synthetase long-chain family member 3 (ACSL3) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ACSL3
• Synonyms: ACS3; FACL3; LACS 3; LACS3; PRO2194
• ACCN: NM_004457
• Insert Size: 1761 bp
• Sequence Data:
  Insert Sequence

  >SC216323 3'UTR clone of NM_004457
  The sequence shown below is from the reference sequence of NM_004457. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ATTACCAGGCGGACATTGAGCGAATGTATGGAAGAAAATAATTATTCTCTTCTGGCATCAGTTTGCTACA
  GTGAGCTCAGATCAAATAGGAAAATACTTGAAATGCATGTCTCAAGCTGCAAGGCAAACTCCATTCCTCA
  TATTAAACTATTACTTCTCATGACGTCACCATTTTTAACTGACAGGATTAGTAAAACATTAAGACAGCAA
  ACTTGTGTCTGTCTCTTCTTTCATTTTCCCCGCCACCAACTTACTTTACCACCTATGACTGTACTTGTCA
  GTATGAGAATTTTTCTGAATCATATTGGGGAAGCAGTGATTTTAAAACCTCAAGTTTTTAAACATGATTT
  ATATGTTCTGTATAATGTTCAGTTTGTAACTTTTTAAAAGTTTGGATGTATAGAGGGATAAATAGGAAAT
  ATAAGAATTGGTTATTTGGGGGCTTTTTTACTTACTGTATTTAAAAATACAAGGGTATTGATATGAAATT
  ATGTAAATTTCAAATGCTTATGAATCAAATCATTGTTGAACAAAAGATTTGTTGCTGTGTAATTATTGTC
  TTGTATGCATTTGAGAGAAATAAATATACCCATACTTATGTTTTAAGAAGTTGAGATCTTGTGAATATAT
  GCCTGTCAGTGTCTTCTTTATATATTTATTTTTTATTAGAAAAAATGAAGTTTGGTTGGTGATGCATGAA
  ACAAAATAGCAAGAGAGGGTTATAGTTTAATAGTAAGGGAGATAACACAGCATGTGTAGCACCAGTTGAT
  AATTGGTCTCTAGTAGCTTACTGTCAAAATGTTCAATGAAGTCTTCTGTTCATCTGTTGAAACTAGGAAA
  ATACCCAAACTTAAATGGAAGAATTCTGAAAGAGAGGATAGAATTTAAAGAACAAGAGTATATAAAGTTA
  TTCTTTGAATATTTCGTTGACTATATGTACATTGAGTTATCTATATTTGTAAACAAATTAGTCATGGAAA
  ATTATTCTATCTCAAAGTCTCCTTTTAGTCTAGATAATCATTATTTCATTTTAAAATTAGTGTTTTTCCT
  AGTTTGCACTGATGCGTGTATGGATGTGTGTGAGTCAGTGGTAGCTTATTTAAAAAGCACCTTATCCTTT
  CTCCCATAACCTTTGTACACTAAAAAATGAAAGAATTTAGAATGTATTTGATGATAGCATTCTCACTAAG
  ACACATGAGAATTTAACTTTATAACCGCGTGAGTTAAGATTTAATTCATAGGTTTTGATGTCATTGTTGA
  AGTTATTTGTAATTCAGAAACCTTGCTTGTGTGATACATAGTCTCTTCATTTATTACTGCTTGTCTGTTG
  TTATATCTGGATTATCAAAAGCAATAGTGCACCAATTAAGATGTGCTCAAATCAGGACTTAAATCATAGG
  CACCACATTTTTCATGTCAGACTAGTTACTTTGTTGATTCTCAGTTACTGTAGGCATCAAAAGGCAAAAA
  TCAAAAAAAAAAAAAACAAAAACAAAAAAAAAGATGAACCTAGGTCTGTGTAAAGTAAGGGGAGTGTTAG
  GAGCAGCCAGGACTGTGTAGTGTGTGTTTGGTTGCATCACAAACATCGTATGTGGAGACATTGCAATACA
  GTGTTTTTTGTTTTCAACTTTTCTTGTATTGTATATTTGTATTATGTTTTGAATGCTTTTCTCTTTTCAT
  AATTAAATATTAATGTTTGGGATAACTGCCAAGAAGAAGTAAAAATATTGAATGGAACTTCTATATGAGG
  ATGCTGTGATC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004457.3
• Summary: 'The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]'
• Locus ID: 2181