ROCK2 (NM_004850) Human 3' UTR Clone

CAT#: SC216436

3`UTR clone of Rho-associated coiled-coil containing protein kinase 2 (ROCK2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ROCK2
• Synonyms: ROCK-II
• ACCN: NM_004850
• Insert Size: 1827
• Sequence Data:
  Insert Sequence

  >SC216436 3'UTR clone of NM_004850
  The sequence shown below is from the reference sequence of NM_004850. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ATTAGACGGCCAAGTCGACAGCTTGCCCCAAACAAACCTAGCTAACTGCCTTCTATGAAAGCAGTCATTA
  TTCAAGGTGATCGTATTCTTCCAGTGAAAACAAGACTGAAATATGATGGCCCAAAATTTATTAAAAAGCT
  ATATTTTCCTGAGAGACTGATACATACACTCATACATATATGTGTTCCCCTTTTCCCTGTAATATAAATT
  ACAAATCTGGGCTCCTTTGAAGCAACAGGTTGAACCAACAATGATTGGTTGATAGACTAAGGATATATGC
  AACTCTTCCAGACTTTTCCATAAAGCTCTCTCGGCAGTCGCTCACACTACAATGCACACAAGGATTGAGA
  AGAGTTAAAGGCTAAAGAAAACATCTTTTCTAGCTTCAACAGAGAGGTTTCACCAGCACATTTACCAGAA
  GAATCTGGGAATGGATTCCACTACAGTGATATTGACTGCATCTTTAAGAAGTGACCATTATACTGTGTAT
  ATATATATAAACACACACACATATATATATATATATATAGTACTCTAATACTGCAAGAAGGTTTTTTAAA
  CTTCCCACTTTATTTTTTATACACATTAATCAGATATCATTACTTGCTGCAGTTGCAACTATGCACTTGT
  ATAAAGCCATAATGTTGGAGTTTATATCACTCATTCCTGTGTACCTGATGGAAGTTGCATGTTCATGTTT
  AAGCAGTTACTGTAACAAGAAGTTTAAAGTTAATTATATCAGTTTCCTAATGCTTCATGATAGGCAACTT
  TACCCATTTTGAATGCCTTAATTTAATTTTTTTCAAAGTCTCAGCCCTGTCTGTATTAAAAAACAAAAAA
  AGCGTTTACCAGCTCTTAGGATGTAAACTAGCTTTGTGGAAGATAAATCGTGCACTATTTTTACACATAA
  ATAGTTATATCAATGTCAGCCTATTTTGATTAACAAATGTTTTTAAAGTATTATTGGTTATAGAAACAAT
  AATGGATGGTGTTGGAACTAATATATCCTTGATGTCTGTCTATTATTCATTCAACTCTTTTTACAGACCT
  CAGTATTAGTCTGTGACTACAAAATATTTTATTTGCTTTAAATTTGCTGGCTACCCTAGATGTGTTTTTA
  TTCCTGGTAAAGACATTTGTGATTACATTTTCACACTTAAGATTCAAAATTTTTCCCAAATATAAAGAAA
  ACTAAGACAGACTGTAGATGCATTTTAAATATTTAAATATGATCCTCAGACATGCAGCTGTGTGTGGCAG
  TATTTTAGTACCGGGTTAAGAAAACTGGCAACTGGGAAGAAGTGGCCTCAAAGGCACTTAATTTGATTTT
  TATTTTTTAAATGCTGTCAAAGTTACAGTTTACGCAGGACATTCTTGCCGTATTCTCATGATCCCAGATA
  AGTGTGTGTTTTATACTGCAACAATATGCAGCAATGGTAAGCGTAAAGTTTTTTTTTTGTTTTTGTTTTT
  TTTTATATTATGAAGTCTTTTAACAGTCTCTCTTTATATAAATACACAGAGTTTGGTATGATATTTAAAT
  ACATCATCTGGCCAGGCATGGTGGCTTACGCCTGTAATCCTAGCACTTTGGGAGGCCAAGACGGGCGGAT
  CACCTGAGGTGAGGAGTTCAAGACCAGCCTGCCCAACATAGTGAAACTCCGTCTCTACCAATATACAAAA
  ATTAGCCGGGCATGATGGTGGTGGCCTGTAATCCCAGCTACTTGGGAGGCTGAGACAGGAGAATCGCTTG
  AACCCAGGAGACGGTGGTTGCAGTGAGCGAAGATCGAGCCACTGCACTCCAGCCTGGGCAGCTGAACAAG
  ACTCCGT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_004850.3
• Summary: The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008]
• Locus ID: 9475