GPC1 (NM_002081) Human 3' UTR Clone

CAT#: SC216447

3`UTR clone of glypican 1 (GPC1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: GPC1
• Synonyms: glypican
• ACCN: NM_002081
• Insert Size: 1793 bp
• Sequence Data:
  Insert Sequence

  >SC216447 3'UTR clone of NM_002081
  The sequence shown below is from the reference sequence of NM_002081. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCCCTTACAGTAGCCAGGCCCCGGTGGCGGTAACTGCCCCAAGGCCCCAGGGACAGAGGCCAAGGACTGA
  CTTTGCCAAAAATACAACACAGACGATATTTAATTCACCTCAGCCTGGAGAGGCCTGGGGTGGGACAGGG
  AGGGCCGGCGGCTCTGAGCAGGGGCAGGCGCAGAGGTCCCAGCCCCAGGCCTGGCCTCGCCTGCCTTTCT
  GCCTTTTAATTTTGTATGAGGTCCTCAGGTCAGCTGGGAGCCAGTGTGCCCAAAAGCCATGTATTTCAGG
  GACCTCAGGGGCACCTCCGGCTGCCTAGCCCTCCCCCCAGCTCCCTGCACCGCCGCAGAAGCAGCCCCTC
  GAGGCCTACAGAGGAGGCCTCAAAGCAACCCGCTGGAGCCCACAGCGAGCCTGTGCCTTCCTCCCCGCCT
  CCTCCCACTGGGACTCCCAGCAGAGCCCACCAGCCAGCCCTGGCCCACCCCCCAGCCTCCAGAGAAGCCC
  CGCACGGGCTGTCTGGGTGTCCGCCATCCAGGGTCTGGCAGAGCCTCTGAGATGATGCATGATGCCCTCC
  CCTCAGCGCAGGCTGCAGAGCCCGGCCCCACCTCCCTGCGCCCTTGAGGGGCCCCAGCGTCTGCAGGGTG
  ACGCCTGAGACAGCACCACTGCTGAGGAGTCTGAGGACTGTCCTCCCACAGACCTGCAGTGAGGGGCCCT
  CCATGCGCAGATGAGGGGCCACTGACCCACCTGCGCTTCTGCTGGAGGAGGGGAAGCTGGGCCCAAAGGC
  CCAGGGAGGCAGCGTGGGCTCTGCCAATGTGGGCTGCCCCTCGCACACAGGGCTCACAGGGCAGGCCTTG
  CTGGGGTCCAGGGCTGTTGGAGGACCCCGAGGGCTGAGGAGCAGCCAGGACCCGCCTGCTCCCATCCTCA
  CCCAGATCAGGAACCAGGGCCTCCCTGTTCACGGTGACACAGGTCAGGGCTCAGAGTGACCCTCAGCTGT
  CACCTGCTCACAGGGATGCTGGTGGCTGGTGAGACCCCGCACTGCAGACGGGAATGCCTAGGTCCCTTCC
  CGACCCAGCCAGCTGCAGGGCACGGGGACCTGGATAGTTAAGGGCTTTTCCAAACATGCATCCATTTACT
  GACACTTCCTGTCCTTGTTCATGGAGAGCTGTTCGCTCCTCCCAGATGGCTTCGGAGGGCCGCAGGGCCC
  ACCTTGGACCCTGGTGACCTCCTGTCACTCACTGAGGCCATCAGGGCCCTGCCCCAGGCCTGGACGGGCC
  CTCCTTCCCTCCTGTGCCCCAGCTGCCAGGCGGCCCTGGGGAGGGGTGGTGTGGTGTTGGGAAGGGGTCC
  TGCAGGGGGAGGAGGACTTGGAGGGTCTGGGGGCAGCTGTCCTGAACCGACTGACCCTGAGGAGGCCGCT
  TAGTGCTGCTTTGCTTTTCATCACCGTCCCGCACAGTGGACGGAGGTCCCCGGTTGCTGGTCAGGTCCCC
  ATGGCTTGTTCTCTGGAACCTGACTTTAGATGTTTTGGGATCAGGAGCCCCCAACACAGGCAAGTCCACC
  CCATAATAACCCTGCCAGTGCCAGGGTGGGCTGGGGACTCTGGCACAGTGATGCCGGGCGCCAGGACAGC
  AGCACTCCCGCTGCACACAGACGGCCTAGGGGTGGCGCTCAGACCCCACCCTACGCTCATCTCTGGAAGG
  GGCAGCCCTGAGTGGTCACTGGTCAGGGCAGTGGCCAAGCCTGCTGTGTCCTTCCTCCACAAGGTCCCCC
  CACCGCTCAGTGTCAGCGGGTGACGTGTGTTCTTTTGAGTCCT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002081.2
• Summary: 'Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq, Jul 2008]'
• Locus ID: 2817