GNB1 (NM_002074) Human 3' UTR Clone

CAT#: SC216471

3`UTR clone of guanine nucleotide binding protein (G protein) beta polypeptide 1 (GNB1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: GNB1
• Synonyms: MRD42
• ACCN: NM_002074
• Insert Size: 1801 bp
• Sequence Data:
  Insert Sequence

  >SC216471 3'UTR clone of NM_002074
  The sequence shown below is from the reference sequence of NM_002074. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGGATAGCTTCCTCAAGATCTGGAACTAACGCCAGTAGCATGTGGATGCCATGGAGACTGGAAGACCATT
  CCAACTTGGACGCGTTACCATGAGAGCATATCCTATCCAACCGTACTAACGTGGACACCCTACACCTCCC
  CTCAGAACTTCAAAAGGGCAAGATCTTTTTTCCTTCACTTATTGCTGAAACCAAGAGCACAATTCCCATT
  GAGAGAAAGATCTCTGTGCTGTAAACTAAAACAAATTGTGCATTCCTTCCGGGGCCATCGTCTTTGTTTT
  CTTTTTTGTCTTGAATGAATTTTAAAAGGAAATATATAATAAAAATGTTAACCAGAAGGTAAACTTGAGT
  GTAATTGTCAGACAGACACACTTTTCCACCAGTGTATTTGAATTTTAGACCAGTGACCCTGTTTTGTGGC
  ATTCATGCAAAACATGCTGAGGGCTTTGTTCATCTGGTCATCGTGTCCAAATTTCAGTCATGTTTGTAGC
  AAGATTTTGGAAGCATTCATATTTCCTTTTTAAAATGTATTCCTTTGTGTTCAACAGTTAATCAAAACCA
  GAGAGTCTAGGGCAGCCTCTCTGATGTTGTCAATGATGTAAATTCAGTCCCTGGTTTTTAATTTTCTGTC
  TGATGTCACAGATCATTGTTGCACACAAACGTGGCATAGAAAAGAACATGTTCAGAAGCCATGGGGCCAA
  GCACATGCGGGGACGGTCTCAAATGCGTGATCAGAGAATCCTTCACCTTTGCTGAAAAGTGAGCTCAGAT
  CCAGCACCATGTTCCTCCTGACCCATCCTGTCTATCTTCTCAGTTGAGTTTTTAATCTCACTTTGGGTTT
  CCTTGTGAAGTTGGAGGGAAGTTTATAATAGCCTAACACTACCCCACCCCCAACTAGGAGGAACCTCTGT
  TTTCAAGAGAGATGCCTGTCCTGTGCTTGGATAGTCAGTCAATTATTTGTGTATGAAACAATGTACAAAT
  CAATGTTTTGAAAATAATGATCTCAGACTTTCTAAGTTAAATTTTAAAAATTTTGATTGTTTGCCATATT
  GGGTGGGTTTACTCTTAGAATCGCATGCTGTAGAAATGCTCAAAAGTGCATATGGGACTCAGTCCTTAGG
  TGTTCTTTTTCTTTTAAGAAATAACCTCTTACAGTTGTAACCATTGCGGCTCTGTCCACTTCTCGTTGCT
  GCTCTGTGGCACATATCGGAAGCAGTACAGCGCGCGGCTCTACACGCTTGGGTAGCGGGATAAGTCACTG
  TTTTCTTTATTTCTTTAAAAAAAAAAAAGTTCTGTTGCAAACGACTGCTGTTGGATTCTGAGGGTGGGGA
  GGGAGAGAGAGGGAGGGAGAGGGAGTGAAGAGCCTGCCCTCCTATATGGATTCTTCAGGGCCCTCCACAT
  CTGAGGTGGCTCATTCCCATCACACACAGATTGTCCTGGTGTTCATTTCAAGGCCAGTGTTCAGCAGCAG
  CGTTTGGAAAGCAGGTTCTGTGGGACCCCCCGCCCCGCCCCCCGCACTCCTTCATAGCAGCAGTAGTGGC
  TTCTCCATCCTGTTTTCTGCAACATTCTATACAAAACTGTGCTGTGACCTTGCGGTAGGCCTGGATCTGG
  CAAAGAGAATACAAATGAAACCCCTTCTTTCTCTTTCCGTCCAACAACTCTGTAGAGCTCTCTGCACCCT
  TACCCCTTTCCACCTTTTGTATTTAATTTTAAAGTCAGTGTACTGCAAGGAAGCTGGATGCAAGATAGAT
  ACTATATTAAACTGTACTGTTATTTAAGATGTAATAAAGCAGTTTGACATG
  
  CGGACCGTTACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-RsrII
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002074.2
• Summary: 'Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]'
• Locus ID: 2782