Prostaglandin dehydrogenase 1 (HPGD) (NM_000860) Human 3' UTR Clone

CAT#: SC216485

3`UTR clone of hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: HPGD
• Synonyms: 15-PGDH; PGDH; PGDH1; PHOAR1; SDR36C1
• ACCN: NM_000860
• Insert Size: 1820 bp
• Sequence Data:
  Insert Sequence

  >SC216485 3'UTR clone of NM_000860
  The sequence shown below is from the reference sequence of NM_000860. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CAAGACTATGATACAACTCCATTTCAAGCAAAAACCCAATGAACAGCTTATGTGTTAGCCATAGCTGAAA
  ATAAGCACAAATAGCTTATATTCAGATCCTATCTTCATTTGAATATAGCTTTTAAATGAAATGTTACAGT
  TTGAAGTTTTCCTTCATGCACTTGGTGATAAACGTTTTCTAAATTTTTAGTTAAGTATATGGATAAAAAG
  TTATGAACTATTAAAAATGTGATGTGGACCAAAGGCTAGGTTGTAATCTTGATAGTCTAAAAAATGATCA
  AAACAAATGATTTTCAAGGAATATTCAATATTCTGCCTTTCAGAAAGTGTATTTATATCTGTGCTTCATA
  AATATTAATGTTCTTCAGAACATCATTTTAAAGGAGATACTTGAATTGTTATTTAAATCAAACCAGATGT
  AAAACACTCACATACAAGTTCATACTTTAAAAGAGGAAAGCTACTTAACAATGACAAATATTTCACAATA
  ATAATTTTTACTTATATACCATCTTTCAACTGAACATTTCAGTTCTTCCAAGAGCTTCTTAGAGTAGTAT
  ATTTTGGGGGCAGTCAAGGAATAAACTACAGTGTAAACATATCCCAGATGAAAACTGCTGTATGGAAAAA
  TGACAGAAAGTAACTGATTGACACTGTTGATTCACAGTTCAGCCTCCTATCTGGGAAAGACATTTCTTTC
  CTCTGCTCACTTTAAGAACTTTTACCGACTCCAAAAATCTCAGGAATTAAACTTTTAACAGTTACAGCAA
  TAAAGAATAGTTAGTACTCCAAAAATATTATATTTAAGATGCTCAACAAGAAAAAAATGCAAATGTAATA
  TTTTTTTCAAATTACTTCTTTATTGACTTGTCCAAATTTCAAAAGTGCCTACCCTTCAATAAAACTTTTT
  TATTCTGATCTCCATAAATTACTTAGTCTTCTATGTATAGCTATCAAGGAAATAAAACCAATTTTGCCAC
  AGCCACAACTGTAAATGTTTTTGTACCCATGCTGAAACTCATAACAACACAGACATAAAAATAGCTGTGA
  GGTTTTGCTTTTTTTGTTGTCAGCTATCTTAAGAATCATTAAATACACCTGCTTTGGGTAAAACTCTTTG
  CAAGCAGTAATTAACACTAGTAACAGTGAAAGCACAAGATTTCCAAATCAGTCGTTTTCTCAAAAAAATA
  TCGTATAAGTGACTCATCCTGTCTGCTAACTCCAGACCTCCCAGCTTGAAGCCAAATCTTTCCATGTGAG
  ATTGATATGGATTTCCTAGAAGTACTGGAATGTTGTCATATCTTGCCCTATTTTAATTCTGCTATAGAAA
  ACAATTGCCTTCACTTTTAAGGAGTAATTTGAATATTAATAACTCTGGTCTAGATTTTCATATAATGTAT
  TAAAGACAAAGTAGTGAACATCAATGAACATCTGATAGAGATAAACTGTAATCAGGCATAAGCTTGTTTG
  TATGTTCTGGCAGTGACTAATCAGTAAATGATGTCGGTTTGCCCAGTATCACTTATCTTCTGTATTTTTC
  CTCTGTCGTGTAAATAGTATAACCTTTTCATTTATGGACAATTTTTTGGACTAGTAGCCTTCAATATACA
  TTCTGCTTTGAATTAATTTTTTCAAATCAATAAATTATGTAGACATTTAAAATCAAATATCAAGTAGAAT
  TGAAAAATGTGAGTTACATAAGTTAAAAACTTACTTTAAATCTTACCTTCTATAGGTAGCTCTAAATAAA
  TTCATATGGTTATATGGCATCTCTGGTGTATACTGATTGAGAAAATAATTAAACTGAAGTTAGGGGAGGG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000860.4
• Summary: 'This gene encodes a member of the short-chain nonmetalloenzyme alcohol dehydrogenase protein family. The encoded enzyme is responsible for the metabolism of prostaglandins, which function in a variety of physiologic and cellular processes such as inflammation. Mutations in this gene result in primary autosomal recessive hypertrophic osteoarthropathy and cranioosteoarthropathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]'
• Locus ID: 3248