CD73 (NT5E) (NM_002526) Human 3' UTR Clone

CAT#: SC216555

3`UTR clone of 5'-nucleotidase ecto (CD73) (NT5E) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: NT5E
• Synonyms: CALJA; CD73; E5NT; eN; eNT; NT; NT5; NTE
• ACCN: NM_002526
• Insert Size: 1789 bp
• Sequence Data:
  Insert Sequence

  >SC216555 3'UTR clone of NM_002526
  The sequence shown below is from the reference sequence of NM_002526. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ACTTTGGGCAGTGATCTTTGTTTTATACCAATAGCCAAAAATTCTCCTTGCCTTTAATGTGTGAAACTGC
  ATTTTTTCAAGTGAGATTCAAATCTGCCTTTTAGGACCTGGCTTTGTGACAGCAAAAACCATCTTTACAG
  GCTCCTAGAAGCTGAAGGTTAGAGCATTATAAAATGAAGAGACAGACATGATTACTCAGGGTCAGCAACC
  TAGTGAGTTAGAAAAAAAATTAACATAGGGCCCTATAAGGAGAAAGCCAACTATGTTAAGTTTACGTGTC
  CAAATTTTAATGAAATTTTACTAACAATTTTAAACCATATTTTTCTTCTTCATATCCATTTCTAATCCAT
  CAAACAGCTTATGTTTACATAAAATTTTATCATTCACAAGGAAGTTTTAAGCACACTGTCTCATTTGATA
  TCCACAACTTATTTTTGGTAGGAAAGAGAGATGTTTTTCCCACCTGTCAGATGAAAAAACTGAAGCTCAA
  AAAGGGTTGACTTGACCATACAGCTAATGCTGACAGATCCAAGACCTAGACCTAGGTCTTTTGAACTCAA
  GTCCAGCATTCTCAACTATATCAAGTTACTGTTCAGAATACTTAATATCTCCTCTCTTCATAATTATCAA
  TAGCCCCAAGCTCATGGATGACAAATCTCTGCTTTATTTCTTGTCTCTATTTTTTCACTTTATAGCTCCT
  GTTATAATAGCAAGTTTAATGGTATAAACACAGGATACCATCCTCTCTTGCAACACCCATGTGCCTTTGA
  TGAGTCAGGTAGCAAGCTGTAGTAGATAATGAGAAAGGCCAGAGGCTGCAAAAGACAGTCAAAGGACACG
  AGAGAAAGGAAGGGGAAGAACAGGACTCCAGGACTGTTTTATATTATAGAAAAGCAAGAGCTAAAGAGCA
  TTTACACATGTTAAACAGATACTTGTTAAGCATAGTGCCTGACACACGGCATTAGCTGTTATTTTATGAG
  ATTCCATCAGCTCTGCCTCTGTCCTCTTTCTTCTAACATGAAGGTATCATGAGAAGAGAACCTTCTAACA
  TAAGCTGTAATTCTAAACCTGCACTTGTCCCTCTCCAGCAAGAGGCTAGCACTGAATTCATTCTACTCAT
  ACTACACACCCAGTTATGGAATGTCCAGAGTTCTCGAAGAAAATAAATGACTTTAGGAAGAGGTATACAT
  TTTTTAAGTCGCTCTGCCTCCAAATCTGAACAGTCACTGTAAATCATTCTTAAGCCCAGATATGAGAACT
  TCTGCTGGAAAGTGGGACCCTCTGAGTGGGTGGTCAGAAAATACCCATGCTGATGAAATGACCTATGCCC
  AAAGAACAAATACTTAACGTGGGAGTGGAACCACATGAGCCTGCTCAGCTCTGCATAAGTAATTCAAGAA
  ATGGGAGGCTTCACCTTAAAAACAGTGTGCAAATGGCAGCTAGAGGTTTTGATAGGAAGTATGTTTGTTT
  CTTAGTGTTTACAAATATTAAGTACTCTTGATACAAAATATACTTTTAAACTTCATAACCTTTTTATAAA
  AGTTGTTGCAGCAAAATAATAGCCTCGGTTCTATGCATATATGGATTAGCTATAAAAAATGTCAATAAGA
  TTGTACAAGGAAAATTAGAGAAAGTCACATTTAGGGTTTATTTTTTACACTTGGCCAGTAAAATAGGGTA
  AATCCTATTAGAATTTTTTAAAGAACTTTTTTTAAGTTTCCTAAATCTGTGTGTGTATTGTGAAGTGGTA
  TAAGAAATGACTTTGAACCACTTTGCAATTGTAGATTCC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002526.2
• Summary: 'The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]'
• Locus ID: 4907