NFAT2 (NFATC1) (NM_172389) Human 3' UTR Clone

CAT#: SC216669

3`UTR clone of nuclear factor of activated T-cells cytoplasmic calcineurin-dependent 1 (NFATC1) transcript variant 5 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: NFATC1
• Synonyms: NF-ATC; NF-ATc1.2; NFAT2; NFATc
• ACCN: NM_172389
• Insert Size: 1799 bp
• Sequence Data:
  Insert Sequence

  >SC216669 3'UTR clone of NM_172389
  The sequence shown below is from the reference sequence of NM_172389. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCCCTGCTGCCACAGCAGTAAATGAAATAATACGAAATGACCTCTCCAGCACGAGCACCCACTCCTAGTT
  GCCACATTGGAGCACTCAGTTCAGCAGGGGTATGCTGACTTCAGCAGACAAAGACTTTTGAATAAATAAA
  CTGAACTCACACCTGGTACCACTCAGAACCTCCAACTGACTGAATGCCAGGAGCTGAACATTAATATGTG
  CAAAGATTGGCTCTCCAACAAGAAGGAAAGCAGGGAGGAAGGGAGACCACTGTGTCACCTGGAGGAGAAG
  TCATCTCATGACAACAGAAGGGAGGTGGCCGGGCTGAGCACGGGAGACCCACCGTGCAGGGGCCTTTCAT
  GGGAACGGCCCACACGCAGTTTGACCCCACGCCCAGCCCTTCTGGCACCCCTGGGGTTCAATACTGGAAG
  TGCCTTATTTAACCAGACCATCAGGGCATCATAGAATTGAGCATTGAATTTGCTACTGTAGGAGTATTTT
  TAGGAGCAGAAACTGCAAACACATTTCATTGTGAGGTTTTACCCTCTGTATGAATGAAGAGAACGCTGGA
  AGGCTGCGAGAGGACTCTAGTATGAGTCTCCAACATTTGGAACGTTTCCTGGGCTGTCACGTACACTCCT
  GCTGCCTTACACAGTGCATTTTAGAATCTTCCAGTCTGTCATCTCAGCTCTTTTGTAACATGCTTCCCTT
  GTCTGCGCGGTTGAAACCGTAGGCTTGTTCATAGTCGCATGCTCGCATCTTTGTTTTTAATCTGGCTTCG
  AACATAGCACAAGTAACTTGAATAGCACATCAATAGGTTACTGGACAAAAGCAGAAAAACCTGTTACAGG
  ATAGCCTGCATTTGCATGTGTGTACATATCTAGGCATCTATTTATGTATAAATAATAACAGAGCCGACGT
  GTCCTCGCCCAGGAGGGCTTCCCTGTCAGCAATAACCGGCATCCGTTTTGGAACCTGCGTCTGGGGCTCC
  AGTCGCTGCTCTTGCTGGCGTCCATCGCCGCCTCGGACGGCCGTGCATTTTCTCGTCTCACGCAGTTCGA
  GGAGGACCCTAGAAAGCCAGGAGCTGTGATTGACAGTAGCTGTAGGTTACCAGACGGCAACATTAGAAAG
  TGATTGTAAATAACATGCAACCTAAGTGTAATATATTTGTTCAGTTATAAGATGATTGTTTCACAGAAGC
  CTTACCACTCTCTGCTTCATCTAAGAAAACCAATACCAAAAACGCCACTTTAATGCTCAGCCCTGCGTTG
  TGTGTTTTCAGATGAGTTACTGTTAACAGGTAGGTTTGTGTAGGCCTTGCTGGGCACTCTGTACAATTAG
  TTGCTTATTACGTATGATTACTCACAGCGATCTATTGTTCCATATAACCAAAAAGCATGGTTTATTCATT
  GAAACACGGTTGACCTGAACTCGTGCCTTAGGAATTAATGCCCCCTTATGGAACCTGCCTGAATTGCACC
  TGCGGGTGGAGGCTCCGGCTGTGAAGTCACTGAACAGAACGTCGCTGATGGAGAAAGGGCTCCCGCAGAA
  GGAACGGCCTGTACCGTGCGCTCCGGCACAATCGCGTCTCTTGTGTCTCACTCACGGAAAGAAACAACCT
  GAAGGCCATCCCGTCGGTCTGCACGTAACCGTGAAGACGTGTGGCCGCGTCCCACCTGCGGCTGGGTACC
  CTGCACCCGGCACTGTAGGAGTCACGTGCAGCCTTTCTCAGGGGACTGTCATTGAAAAGGAAACGTTTGA
  TGTCTGTGTCAGCTGTCTTTGTAGTTAGGAAATAGATCCAATAAAGCCG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_172389.1
• Summary: 'The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes. [provided by RefSeq, Jul 2013]'
• Locus ID: 4772