PTP1B (PTPN1) (NM_002827) Human 3' UTR Clone

CAT#: SC216694

3`UTR clone of protein tyrosine phosphatase non-receptor type 1 (PTPN1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PTPN1
• Synonyms: PTP1B
• ACCN: NM_002827
• Insert Size: 1847 bp
• Sequence Data:
  Insert Sequence

  >SC216694 3'UTR clone of NM_002827
  The sequence shown below is from the reference sequence of NM_002827. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTCTGCTACAGGTTCCTGTTCAACAGCAACACATAGCCTGACCCTCCTCCACTCCACCTCCACCCACTGT
  CCGCCTCTGCCCGCAGAGCCCACGCCCGACTAGCAGGCATGCCGCGGTAGGTAAGGGCCGCCGGACCGCG
  TAGAGAGCCGGGCCCCGGACGGACGTTGGTTCTGCACTAAAACCCATCTTCCCCGGATGTGTGTCTCACC
  CCTCATCCTTTTACTTTTTGCCCCTTCCACTTTGAGTACCAAATCCACAAGCCATTTTTTGAGGAGAGTG
  AAAGAGAGTACCATGCTGGCGGCGCAGAGGGAAGGGGCCTACACCCGTCTTGGGGCTCGCCCCACCCAGG
  GCTCCCTCCTGGAGCATCCCAGGCGGGCGGCACGCCAACAGCCCCCCCCTTGAATCTGCAGGGAGCAACT
  CTCCACTCCATATTTATTTAAACAATTTTTTCCCCAAAGGCATCCATAGTGCACTAGCATTTTCTTGAAC
  CAATAATGTATTAAAATTTTTTGATGTCAGCCTTGCATCAAGGGCTTTATCAAAAAGTACAATAATAAAT
  CCTCAGGTAGTACTGGGAATGGAAGGCTTTGCCATGGGCCTGCTGCGTCAGACCAGTACTGGGAAGGAGG
  ACGGTTGTAAGCAGTTGTTATTTAGTGATATTGTGGGTAACGTGAGAAGATAGAACAATGCTATAATATA
  TAATGAACACGTGGGTATTTAATAAGAAACATGATGTGAGATTACTTTGTCCCGCTTATTCTCCTCCCTG
  TTATCTGCTAGATCTAGTTCTCAATCACTGCTCCCCCGTGTGTATTAGAATGCATGTAAGGTCTTCTTGT
  GTCCTGATGAAAAATATGTGCTTGAAATGAGAAACTTTGATCTCTGCTTACTAATGTGCCCCATGTCCAA
  GTCCAACCTGCCTGTGCATGACCTGATCATTACATGGCTGTGGTTCCTAAGCCTGTTGCTGAAGTCATTG
  TCGCTCAGCAATAGGGTGCAGTTTTCCAGGAATAGGCATTTGCCTAATTCCTGGCATGACACTCTAGTGA
  CTTCCTGGTGAGGCCCAGCCTGTCCTGGTACAGCAGGGTCTTGCTGTAACTCAGACATTCCAAGGGTATG
  GGAAGCCATATTCACACCTCACGCTCTGGACATGATTTAGGGAAGCAGGGACACCCCCCGCCCCCCACCT
  TTGGGATCAGCCTCCGCCATTCCAAGTCAACACTCTTCTTGAGCAGACCGTGATTTGGAAGAGAGGCACC
  TGCTGGAAACCACACTTCTTGAAACAGCCTGGGTGACGGTCCTTTAGGCAGCCTGCCGCCGTCTCTGTCC
  CGGTTCACCTTGCCGAGAGAGGCGCGTCTGCCCCACCCTCAAACCCTGTGGGGCCTGATGGTGCTCACGA
  CTCTTCCTGCAAAGGGAACTGAAGACCTCCACATTAAGTGGCTTTTTAACATGAAAAACACGGCAGCTGT
  AGCTCCCGAGCTACTCTCTTGCCAGCATTTTCACATTTTGCCTTTCTCGTGGTAGAAGCCAGTACAGAGA
  AATTCTGTGGTGGGAACATTCGAGGTGTCACCCTGCAGAGCTATGGTGAGGTGTGGATAAGGCTTAGGTG
  CCAGGCTGTAAGCATTCTGAGCTGGGCTTGTTGTTTTTAAGTCCTGTATATGTATGTAGTAGTTTGGGTG
  TGTATATATAGTAGCATTTCAAAATGGACGTACTGGTTTAACCTCCTATCCTTGGAGAGCAGCTGGCTCT
  CCACCTTGTTACACATTATGTTAGAGAGGTAGCGAGCTGCTCTGCTATATGCCTTAAGCCAATATTTACT
  CATCAGGTCATTATTTTTTACAATGGC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002827.2
• Summary: 'The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013]'
• Locus ID: 5770