Glycerol kinase (GK) (NM_000167) Human 3' UTR Clone

CAT#: SC216798

3`UTR clone of glycerol kinase (GK) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: GK
• Synonyms: GK1; GKD
• ACCN: NM_000167
• Insert Size: 1902 bp
• Sequence Data:
  Insert Sequence

  >SC216798 3'UTR clone of NM_000167
  The sequence shown below is from the reference sequence of NM_000167. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGTTACAACTCAATCTCCAGAAAGTGGTATTCCATAAAACCTACCAACTCATGGATTCCCAAGATGTGAG
  CTTTTTACATAATGAAAGAACCCAGCAATTCTGTCTCTTAATGCAATGACACTATTCATAGACTTTGATT
  TTATTTATAAGCCACTTGCTGCATGACCCTCCAAGTAGACCTGTGGCTTAAAATAAAGAAAATGCAGCAA
  AAAGAATGCTATAGAAATATTTGGTGGTTTTTTTTTTTTTTAAACATCCACAGTTAAGGTTGGGCCAGCT
  ACCTTTGGGGCTGACCCCCTCCATTGCCATAACATCCTGCTCCATTCCCTCTAAGATGTAGGAAGAATTC
  GGATCCTTACCATTGGAATCTTCCATCGAACATACTCAAACACTTTTGGACCAGGATTTGAGTCTCTGCA
  TGACATATACTTGATTAAAAGGTTATTACTAACCTGTTAAAAATCAGCAGCTCTTTGCTTTTAACAGACA
  CCCTAAAAGTCTTCTTTTCTACATAGTTGAAGACAGCAACATCTTCACTGAATGTTTGAATAGAAACCTC
  TACTAAATTATTAAAATAGACATTTAGTGTTCTCACAGCTTGGATATTTTTCTGAAAAGTTATTTGCCAA
  AACTGAAATCCTTCAGATGTTTTCCATGGTCCCACTAATTATAATGACTTTCTGTCTGGATCTTATAGGA
  AAAGATACTTTCTTTTTTCTTCCATCTTTCCTTTTTATATTTTTTACTTTGTATGTATAACATACATGCC
  TATATATTTTATACACTGAGGGTAGCCCATTTATAAATTAAGAGCACATTATATTCAGAAGGTTCTAACA
  GGGCTGGTCTTAAGTGAACCACTGTGTATATAAATATGTTGGAAAACAGCTGTATACATTTTTGGGCAAC
  GGTTATGCATAATATTTACCAGGAGAATTTTTTTCTTAACAAGCCAACATTTAAAATTTATGTTTTATGT
  CAATAAAAGAAAATATACTTTATTGTGACTTCAACTATATTTCTTATCCCTTACATTTTTATTTAATTGT
  CTTAGCTTAAAAAAAGAAGAAACTGTGGAATACTACAGTAAATATTGTTTTCAAACACAAGCAATAATTC
  AAATAGTTATTTTTCTTTTGAATTAATTTTAGACATATTTTGGATCCTATTGAGGGGATAAGAGGATGTC
  AAAAAAGTTAAATACCTAAGTAGAAAAAAATATAGAAATAAAGCCAAGAATCTCTTTCAGTTCAAATGTT
  ATCAATTGTTAATAAGAAATTGCTATCTGGGATGACAGAATTACCTCTGCTTAGTATCTCATTATAACTG
  AAAGAAGGTTTATCATTACAAATACCTTCCAATGAAACCAAGAATTTCTCAAAATATTTAATGTCACATA
  TTATAAGAAGTTACCTAATCCTGCTTCTTAACATCAATTTTTAAAAATATCTTAAAATTACTTTGTTTTG
  TAGTAAACAGTGAAGAAAAGATTGCCTCCTAATTATTTTTTTCAATGAGTGCTGAATGGGAAAACATTTA
  TATCTTACTATAAAAGGTTCTGTTTTGTTTGGAATCAATGGTAGCTTTATTGACTGTTCTGATTGTGCTG
  TTTCTAATTTATTGAATCTGCTAGGTTTTATTGATGCAGCCACCACTTAAGTGACATAAATATTATAGAA
  AGGTACTGTGAAATGATCACTTTGTGGCAGGGGTACTTTTAAACATAAATGTTTCTACAAAAGTAGGTTG
  AGTTCATTGTAAATAATTGTGAAAGCCACTGTTCAAATAATTTTAAGATTACATTAATTTTTCTATAAAT
  TGGAAGATTTATAAATGTTTGAAATTGTACACATTGATATTTAATGACAAATTTACTTAAAATAAATTGA
  CCCCTTGTTCTT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000167.4
• Summary: 'The protein encoded by this gene belongs to the FGGY kinase family. This protein is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Mutations in this gene are associated with glycerol kinase deficiency (GKD). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]'
• Locus ID: 2710