ACACB (NM_001093) Human 3' UTR Clone

CAT#: SC216841

3`UTR clone of acetyl-Coenzyme A carboxylase beta (ACACB) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ACACB
• Synonyms: ACC2; ACCB; HACC275
• ACCN: NM_001093
• Insert Size: 1883 bp
• Sequence Data:
  Insert Sequence

  >SC216841 3'UTR clone of NM_001093
  The sequence shown below is from the reference sequence of NM_001093. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TCGTTCACCTGCTGTCTACCATGGACAGCCCGGCCTCCACCTGACCGTGGCCCGCCCAGCCACTCCCGGG
  ACCACGGCAAAAGGAACCACCCAGACCCACCACCCGTACACCCTCAGCAGACCCTGAAGACTTGCTTTTA
  AACAAAGAAAATCCTGGGCACTTCTGCAGGGCTGCTGGTTCCGAGCTGACACCCGTCTTAACAAAAGGCC
  CAGGAGTGCCTCTTCCAAACAAAAACAGCCTCCTCTCCATAGCTGGGAAGTTTATTTTGTTTTGTCTCTG
  AAGACAGCAGTTTTATTGCATCACTAAATCTAATCAAGCTAAAACATCCCTGTTTCCTTTTGCAAAACAG
  TGCCTGGCATGTGGGATCCAGGCGTTCTTTAGGATCCTTGGATACCACATCGTGAAATCTTTTATTTTTT
  TACTCTGAGACCAGCACCAGATGTAAGTAAGCATCTCATATATTTCAGCCAAATAAATGGGCCAAGGGAA
  AAAAATATATATATATAGACAGGACTAGAGAAAACCTATTTTTGTAATGATGTTTCTTTGGATACTGTCT
  AGTCACCCAGAAAAATGTATGGATGAATTTTTTTTTTTTTTTTTGAGACAAAGTCTCACTGTGTCATGCA
  GGCTGGAGTGCAGTGGCATGATCTCACTGCAACCTCCATCTCCTGTCTCAGCCTCCTAGATAACTGGGAT
  TACAGGTGCCCACCACCATGCCCGGCTAATTTTTGTATTTTTGGTAGAGACAGAGTTTCACCAGGTTGGT
  CAGGCTGGTCTCAAACTCTTGACTTCAGGTAATCCACCCACCTTGGCCTCCCAAAGTGCTGGGATTACAG
  GCATGAGCCACCATCTTCAGCCAGATGATTTTTTTATTGAGAGAGTGAAATGCTATTTTGTTCCCCAAAT
  GGCGCTAGTGAATCACTAGGAGGGTCCCACTGATAGGCCATGTTTAGCACTGGTTGCCAGGGATTCTCTT
  TTTGAGAGAGGGAAAGCAAAATGAATGGAAGTACCCAGCTGGAGGTTTCAGGGCTTCTGGAGGATGCTCT
  CGCATAGCTCGAGGTCCTCTGCCCACCTCTTCTCTCCAAGGAAAATGAGGACTGCCCCTTCCCCCTGCAG
  GATTGGCCCCCAGCCTGCGCATGCACCCTCCTCTTGCCCAAGTGGGGAGCACAGAGGCGGAGAGGAATCC
  CTTACCACACCCACGGCCCAGCTTGCTCACGAGTGTCACCTCTGTGACGGTCACCACTGCTCCCTTGGAG
  GGCCACTTGAGTTACTGTTGCTTCCTCGCCTGCTGGCTTGATGAGCACCGATGGTGGGATCTGACCCCGA
  GGGGCAGAGCTGTCGGTGACTGAGGACTGGACTGTGGTGACCATGCCGATTTGCTCAGGGAGAACGTTGC
  AATGCACCCAGCAGCTCCTGGCTCTGCAGGCGGCACAGCCTGGGGCCCTGTGATCCTCTGGTTTCTTCCA
  TTGGGGCGGAGTCGGGGATGGAGGGAGCTGGCCACAACCCACTGCTCTGATGGGTGGTTTGTCCAAGGAT
  GCTGAATGTAATGCCTGGTCAATGTGGAAGCCCATGAGGTTGCCCAGGGAAGCCTCCAAAAGCTGGGATG
  CTTGAGGGTATCCAAGTTGAAAAAGACAAAATCTGACCATCAGCCAGTGACAGTCCTGGCAAATGAAGGT
  GGGGCGGGGCAGTGAGGGGTGGGAGAAGGTGAATGATTCATTATTCCACCCCGAGGTTTGCTGGGGTGAG
  GGGAAGAATCGATGCTGCTTTGGGAACTGAAGGTTTTTCTGTTGGGAAGGCCCTCTTGGTTTTGGAGAGA
  AAGACAAGTTATGAGTAGCTGCTACCCTGGAACGGTGGGCAGAGAGCCTACTAGGAAATGTGC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001093.3
• Summary: ' Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. ACC-beta is thought to control fatty acid oxidation by means of the ability of malonyl-CoA to inhibit carnitine-palmitoyl-CoA transferase I, the rate-limiting step in fatty acid uptake and oxidation by mitochondria. ACC-beta may be involved in the regulation of fatty acid oxidation, rather than fatty acid biosynthesis. There is evidence for the presence of two ACC-beta isoforms. [provided by RefSeq, Jul 2008]'
• Locus ID: 32