UBE3A (NM_000462) Human 3' UTR Clone

CAT#: SC216901

3`UTR clone of ubiquitin protein ligase E3A (UBE3A) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: UBE3A
• Synonyms: ANCR; AS; E6-AP; EPVE6AP; HPVE6A
• ACCN: NM_000462
• Insert Size: 1905 bp
• Sequence Data:
  Insert Sequence

  >SC216901 3'UTR clone of NM_000462
  The sequence shown below is from the reference sequence of NM_000462. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCATCACGTATGCCAAAGGATTTGGCATGCTGTAAAACAAAACAAAACAAAATAAAACAAAAAAAAGGAA
  GGAAAAAAAAAGAAAAAATTTAAAAAATTTTAAAAATATAACGAGGGATAAATTTTTGGTGGTGATAGTG
  TCCCAGTACAAAAAGGCTGTAAGATAGTCAACCACAGTAGTCACCTATGTCTGTGCCTCCCTTCTTTATT
  GGGGACATGTGGGCTGGAACAGCAGATTTCAGCTACATATATGAACAAATCCTTTATTATTATTATAATT
  ATTTTTTTGCGTGAAAGTGTTACATATTCTTTCACTTGTATGTACAGAGAGGTTTTTCTGAATATTTATT
  TTAAGGGTTAAATCACTTTTGCTTGTGTTTATTACTGCTTGAGGTTGAGCCTTTTGAGTATTTAAAAAAT
  ATATACCAACAGAACTACTCTCCCAAGGAAAATATTGCCACCATTTGTAGACCACGTAACCTTCAAGTAT
  GTGCTACTTTTTTGTCCCTGTATCTAACTCAAATCAGGAACTGTATTTTTTTTAATGATTTGCTTTTGAA
  ACTTGAAGTCTTGAAAACAGTGTGATGCAATTACTGCTGTTCTAGCCCCCAAAGAGTTTTCTGTGCAAAA
  TCTTGAGAATCAATCAATAAAGAAAGATGGAAGGAAGGGAGAAATTGGAATGTTTTAACTGCAGCCCTCA
  GAACTTTAGTAACAGCACAACAAATTAAAAACAAAAACAACTCATGCCACAGTATGTCGTCTTCATGTGT
  CTTGCAATGAACTGTTTCAGTAGCCAATCCTCTTTCTTAGTATATGAAAGGACAGGGATTTTTGTTCTTG
  TTGTTCTCGTTGTTGTTTTAAGTTTACTGGGGAAAGTGCATTTGGCCAAATGAAATGGTAGTCAAGCCTA
  TTGCAACAAAGTTAGGAAGTTTGTTGTTTGTTTATTATAAACAAAAAGCATGTGAAAGTGCACTTAAGAT
  AGAGTTTTTATTAATTACTTACTTATTACCTAGATTTTAAATAGACAATCCAAAGTCTCCCCTTCGTGTT
  GCCATCATCTTGTTGAATCAGCCATTTTATCGAGGCACGTGATCAGTGTTGCAACATAATGAAAAAGATG
  GCTACTGTGCCTTGTGTTACTTAATCATACAGTAAGCTGACCTGGAAATGAATGAAACTATTACTCCTAA
  GAATTACATTGTATAGCCCCACAGATTAAATTTAATTAATTAATTCAAAACATGTTAAACGTTACTTTCA
  TGTACTATGGAAAAGTACAAGTAGGTTTACATTACTGATTTCCAGAAGTAAGTAGTTTCCCCTTTCCTAG
  TCTTCTGTGTATGTGATGTTGTTAATTTCTTTTATTGCATTATAAAATAAAAGGATTATGTATTTTTAAC
  TAAGGTGAGACATTGATATATCCTTTTGCTACAAGCTATAGCTAATGTGCTGAGCTTGTGCCTTGGTGAT
  TGATTGATTGATTGACTGATTGTTTTAACTGATTACTGTAGATCAACCTGATGATTTGTTTGTTTGAAAT
  TGGCAGGAAAAATGCAGCTTTCAAATCATTGGGGGGAGAAAAAGGATGTCTTTCAGGATTATTTTAATTA
  ATTTTTTTCATAATTGAGACAGAACTGTTTGTTATGTACCATAATGCTAAATAAAACTGTGGCACTTTTC
  ACCATAATTTAATTTAGTGGAAAAAGAAGACAATGCTTTCCATATTGTGATAAGGTAACATGGGGTTTTT
  CTGGGCCAGCCTTTAGAACACTGTTAGGGTACATACGCTACCTTGATGAAAGGGACCTTCGTGCAACTGT
  AGTCATCTTAAAGGCTTCTCATCCACTGTGCTTCTTAATGTGTAATTAAAGTGAGGAGAAATTAAATACT
  CTGAGGGCGTTTTAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000462.2
• Summary: 'This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined. [provided by RefSeq, Jul 2008]'
• Locus ID: 7337