ARFGAP1 (NM_175609) Human 3' UTR Clone

CAT#: SC217021

3`UTR clone of ADP-ribosylation factor GTPase activating protein 1 (ARFGAP1) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ARFGAP1
• Synonyms: ARF1GAP; HRIHFB2281
• ACCN: NM_175609
• Insert Size: 1924
• Sequence Data:
  Insert Sequence

  >SC217021 3'UTR clone of NM_175609
  The sequence shown below is from the reference sequence of NM_175609. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ATGATGGCTGGGACAACCAGAACTGGTAGGGCCCACTGCGCCCCCGTCCCCAGCGCCCCCGGGCGACTTC
  GTGTTTGCACTCTGCCCTCGTCGTTCCTCCTCCTTCCATTTGACCCAAGAATCAGCAACTGCAGTGTGAG
  GACAGCGTCTCGGGAGGCAGGACCCTAGGGAGACCCGGGTGTGCGCCGCCTGCGCGTGGGGAGTCTTCGG
  TGCGTGGGGGCGGCTTGCTGTCCAGCCTGTGTGGGGGCCGTCCCGTCCCACACTCCCCTGGGCATTCTTG
  GACTCAAGGCCGGGGCTCTGCGTGGCTTGCTGGGAGGTGGGCTGCAGCACAGAGGCCTGTGACTGCGTTC
  CAGCGGCCAGTTCACTACGCAGTATCTCTGGGGCCTGGGACCAGCCACGTGCCGAGCTGTCAGCGACGTG
  AGGTGTCCCTTCTCGTTGAGATATTTAACTTTGGTTTTGCTCTAGTTCTTTCTTTTTGAAGAGAGTGACT
  GGAGTGGTAAAGATGGAAATGCTGGAAATGATACTGGCGCTCACGCTGCCATCCGACCACCCTCGGCTCC
  CGAGTCCACGCCTGCCTGGGCCTGTGCTGTCAGACCCGCGTCGGTCGTAACCCTCTGTGGCTCCCCTGCA
  TCAGCACCGTCCCACCACCAAGTTCACCAGGTTCACCAGACACGGCCTCCACAATAGCCACACCCACACC
  TGAGCTGTTCTCAGTGCTGGAACTTGACCATCCTGGAACACCCTGGAAGAAAAAGGAGCGCAGGGTGGGC
  CCTCGGCCCTGATGCAGGAGGGTGCGATAGCGGACGTGGCCAGGCAGGAGGGGCCGGGTTCAGGAGCTGA
  GCAGGGGATGCCTGTGCGTGGTGCCTGGGTCTAGGGAAGCTCCAGCCCCAGGATGGGGCTGCCCTGCACA
  CCGGTGCCCGCCACATGCCAACCCTCACCTCCCCGAGGACTGGATGATGTGCTGCCACGTGTGACTCGTC
  TCCCTTGTCTGCCCTGTGTGACCCTCAGTCTTGGCCAGCCATGCATGCGCCCGAAGCTCGTGCAGTTTGT
  ACGTGAGGTGCTCTCCTCCCTGCCACCATGCTCATCACTCTGGCCTTGGCCATGCTCCCTGGTCACCCCA
  CTTCCCGGTCGCCGTCTGCAGCACTCCTGGAGCAGCCTGGGCCCTTCAGCCCCTGTGCTCGTCCCACCCT
  AGGGACTCAGCCACTTGCAGAACAGGATGGGACCGAGATTTCAGCGAGCCCTCCTGGCGCCCGGTCCTCC
  CTGTGGGCACCAGCCCTCTTGGTAGCTGGTGTGGAGGGCCGGTGTCCTTGGCTGCCACGGAGGGATTTGA
  TCACCGAAGCAGCCACCTGCTGTAGTTGGACCTGAGGTCAGAGGCGGGGCATCAGAGGCTCAAGGTGCTG
  AGAAGCCACCGGGAAAGCAGCCAGCACAAAGGGCCCAGGAAGCCAGCCCCCGAGAGCTGAGCGTGGGGGT
  CTTTGAGTGTCTTTCTCCAAGCTGAGACGTGGGCGGCCGCGTGGTATCTCCCGAGGGCTGCTTGGACCCT
  GGTGGGCTGAGTGCTCCGAGGAGGGGTGGACTCCACCTTGGACAGTGGGATGTGGTGTTCCACATGTGCC
  TGTTTCCACGCCAGCACCTTGACTTGGCAGCATGGAGCCAAGGTCTGTCCCCGCCCAGGAGGGTGCCTTC
  CTCGGGGGTAGGGGGACGGCCCACTCTGCCCCAGGGAGTCCCTTTTGATGGGAAGTGCAGTCAGCAGCGT
  GGAGGTGTCTGGGCCACCTTCAGAAGGTGGATGTGGTGGCCGAGACCCCGTCCACGGAGGGTGATGGCCT
  TTCCCTTCTGCAGGTGCGGGCAGGTGGGCCTGGGACCGGTGCTGGGGCCTCTCCTTGCTGTGTGTGAGGG
  CCCAGGTGGAAGGCGCGGACCTGACAGCATTCCA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_175609.1
• Summary: The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
• Locus ID: 55738