CYB5R3 (NM_001129819) Human 3' UTR Clone

CAT#: SC217089

3`UTR clone of cytochrome b5 reductase 3 (CYB5R3) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CYB5R3
• Synonyms: B5R; DIA1
• ACCN: NM_001129819
• Insert Size: 1932 bp
• Sequence Data:
  Insert Sequence

  >SC217089 3'UTR clone of NM_001129819
  The sequence shown below is from the reference sequence of NM_001129819. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CGCTGCTTCGTCTTCTGAGGGCCGGGCACGGTCACACGGCCACCCGCCCCGCGCACCCCACGCCCTGTTC
  ACGCTCACCCAGTCACCTCCCCACATCGCACACTGGGGCCCCGGGTTCAGCCTGGCCTGCCCGTGCCCTG
  GTGAATCACCTGGCTGAGCAGTTCCCCTGGAGCCCCTTCGGGAGCAGGGCTGTGTCCCAGATGGGCCACG
  GCTGAGCCTTCAGAGTACGTCCTGCCTGGCACTTACTGGTCCTTACCAGAGACGCCCAGCCCCATCCCTG
  TCCTCATGACCCCTCGTCCACCCCCCACACACACTATAAGGCTGAGGGCTGCCAGCAGCCCCGTCTGCCC
  ACCATTCCCGGCCGTGGACCATAGTCGGGATGTCAGCAGACACACATGGGCAGCCCAAAGCTGCAGGTGC
  CAGGGCCCACCCCAGCCTCGCCTGTCACCCCCACTCCCGCCTCAGGGCCAGGCCCAGGCCTCACCACCTG
  ACGCTGCATGAGACATTGACACCAGAAAGCCCTCTTGGGGGCACTGCTCCCTACCCCAGGGCCCTGGCCA
  GCCGGGAGCTTGGCTCTCCTCTGGCTAGAGTGGGAAGAGGGGGCTGGCCATGGGGCCCTCCCAGAACCTC
  AGCATTTCCTTCCAGCCCATCCAAACACTGAGGCAGCCTTGGGGAACCCCGAGCTGGGGGGTTGGCAGCC
  CACTGCACCGCCTCAGGGTTTTGGGGTCCTGGGCTGGGGCCACCATCCCTGATGGCAGAACTCCCACAAC
  CACATGTATTTATTCCTCTGTCCTAAACCGTCCCCTCCTTCCCTCACCCCCAGCACAGGGGGATTCTGAG
  CAGTGCCTCTTGTCTGAGGGACATATCAGTGACCTCGACGTTGCCTTTAGACTACAGTTGTGTTAGCCTC
  TTGCGTATTGGCTTTTTCAGAGTCATTTATGAGCAGAAAAAAAAAAAGTAAAACTTTGCTAATATTAACC
  CTTCTCTAGCTCCTCGAGGGTCTGTGACCTGCAACACAAGGGGTGGGGTCAGGAAAGGGCTGGGGAAGAC
  CTAGCATTTTTTTTTTCTTTTTTTTTTTTTTTTGAGACGGAGTCTCCCTTTGTCACCCAGGCTGGAGTGG
  CATGATCTCAGCTCACTACAACCTCCACCTCTCGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTA
  GCTGGGACTAAAAGTGCCCACCACCACACCCAGCTAGTTTTTGTATTTTTTTTTTTTTTTTTTTGAGACG
  GAGTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGGGATCTCGGCTCACTGCAAGCTCCGCCTCCCG
  GGTTCACGCCATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGACTACAGGCGCCCGCCACTACGCCCGGC
  TAATTTTTTGTATTTTTAGTAGAGACGGGGTTTCACCGTTTTAGCCGGGATGGTCTCGATCTCCTGACCT
  CGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACTGCGCCCGGCTCCAGC
  ATTTATTTCTGATGTATCTTTGTGGTAGAAAATTTGGAAAGTGCAGAGAAGTATACACAGGAAGAAAAAT
  TCCCAACCCCCAGAGGCAAACCAGCTGAAACCACGCAACCCCAGTCACCCCAATGCACCGCGAGGCTGCT
  GCCTCCTGTCAGGGTCAGATGAGCCTCGAGGCTCAGGAAAGTCAGAGGATGCCATCTGCATGGTGGTAAA
  TTACAGAGGTGATGAGGCAAGGTGGGTGTGGGGCTGTTCTTAAAACGGGGCAGCAGGAAGGCCCCAAGGA
  GATGGATTTGGGCTGGGACGGGAAGAGAGAGCTGGCCATGCTGGGGTGGGTGGGTGTTCAAATGGTGGAA
  ACAGCAGACGCAAAGGCCCTGCCGTTGGAACCAGCTTGTGGA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001129819.2
• Summary: 'This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias. [provided by RefSeq, Jan 2010]'
• Locus ID: 1727