KCNK2 (NM_001017424) Human 3' UTR Clone

CAT#: SC217168

3`UTR clone of potassium channel subfamily K member 2 (KCNK2) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: KCNK2
• Synonyms: hTREK-1c; hTREK-1e; K2p2.1; TPKC1; TREK; TREK-1; TREK1
• ACCN: NM_001017424
• Insert Size: 1971 bp
• Sequence Data:
  Insert Sequence

  >SC217168 3'UTR clone of NM_001017424
  The sequence shown below is from the reference sequence of NM_001017424. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ACACTGTGCTGGTGAAGAGATTGCTGTGATTGAGAACATCAAATAGCCCTCTCTTTAAATAACCTTAGGC
  ATAGCCATAGGTGAGGACTTCTCTATGCTCTTTATGACTGTTGCTGGTAGCATTTTTTAAATTGTGCATG
  AGCTCAAAGGGGGAACAAAATAGATACACCCATCATGGTCATCTATCATCAAGAGAATTTGGAATTCTGA
  GCCAGCACTTTCTTTCTGATGATGCTTGTTGAACGGTCCACTTTCTTTGATGAGTGGAATGACAAGCAAT
  GTCTGATGCCTTTTTGTGCCCAGACTGTTTTCCTCTCTCTTTCCCTAATGTGCCATAAGGCCTCAGAATG
  AATGAGAATTGTTTCTGGTAACAATGTAGCTTTGAGGGATCAGTTCTTAACTTTTCAGGGTCTACCTAAC
  TGAGCCTAGATATGGACCATTTATGGATGACAACAATTTTTTTTTTGTAAATGACAAGAAATTCTTATGC
  AGCCTTTTACCTAAGAAATTTTCTGTCAGTGCCTTATCTTATGAAGAAACAGAACCTCTCTAGCTAATGT
  GTGGTTTCTCCTTCCCTGCCCCCACCCCTAGGCTCACCTCTGCAGTCTTTTACCCCAGTTCTCCCATTTG
  AATACCATACCTTGCTGGAAACAGTGTGTAAAATGACTGAAGTGATGATGCCCGAAGATGAAATAGATGC
  CAAATTAGATGGACATTGAAGCAACACTCAGCGTTGCCTAGCGTTAAAGGCACTGCAGAGAAATGAGGTG
  CAGAGGTGGCCCCTCTGAGTATTTATTTGACTCAGGTACCAGTGGTACATATATACAGTGTAATTATGAC
  CAGGCTGGTAAAATTGGCTGCTCGCAAACAATCCCCTTTTTTCCTGGCAGTATTTGGAATTTATCATTTA
  TTAATAACTATACATTTTTAAAGGCAGAAGAAGAAAATCTATCTATCTATCTATCTATCTATCTATCTAT
  CTATCTATCTATCTATCTAAATGACCTGACAGAAGAAAACTGTTAAAAATGGATATTATTGGAGGGGATT
  TAAAACAGTGGGTGTGAATTATCATTCTGATGGAAAGAAAATAGCAAAACAATGTGTTACAAGTATTTGC
  TAATAAACAGTATACTGCCAGCTTCTAATTGCTTTTTGATGTATGAAAGGCTTATATAATTTTCTTTTCG
  TTGGGTGACTTTTGCCAGATGAGAGGAGGTGGCACAGTGGTGAGTGCAGGGCACAGTCCTAGCCTTCTGT
  GGGTATACTTTTGGAGTTGTGACTTGGCTGTGAGGGCAGAAGTTGAAGTTGGGATCACTGTGACTTTGCA
  CATGGAAAAATGCAGATTGCAGGCATAATTCATCTCTGACATTAGAGAAAAAGCTGTTATAGCACAATTT
  AAATTTTGAGAGTTTGCTGTGTTTTTTTTTCACATAAAAGAGGCTGATTATTCTTTTTAGTTTAATTTTA
  TATCCTGTAATTCTTTGGATGGTTCCAAGATTCAGAAAAAATTCAGTAAATGCACCCCGTAAATTGCTAC
  CCTTTCCTTTATTTTCATACTTAGATCTGCTGTACATTGTATATATATATAATTTTTAAAATGCAGAAAG
  AAAATAATTTCCCTAAATATAATTGCAAACTGATTTCTTTTACTTTTTTGTGTGTGGGGGTGGGAGCTGT
  ATCTGAATAAGTGGCATTCAGATTAGGGTCTTGAAAAATAAACCCAGAATCTTTAAAAGAAGCAAATAAA
  CTAATAGACGCTTATTTTCCAAAATTTAAATTTAAGCTAGAAATGTAAATATTCAATTAATTTGTTAAAA
  GTACTTTTATAAAGTTAAAAAAAATCCAACCAAAATTTTAGAAAGTCAGGCTCTTTTAGAAAGAAAGCTA
  CACCCATTTCCTCAAATAACTGTTCCGAAAATTTATATGGTGGAATGCGCCATGTATAAACTGTGAATTG
  TATTGACAAAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001017424.2
• Summary: 'This gene encodes one of the members of the two-pore-domain background potassium channel protein family. This type of potassium channel is formed by two homodimers that create a channel that leaks potassium out of the cell to control resting membrane potential. The channel can be opened, however, by certain anesthetics, membrane stretching, intracellular acidosis, and heat. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
• Locus ID: 3776