GCLM (NM_002061) Human 3' UTR Clone
CAT#: SC217196
3`UTR clone of glutamate-cysteine ligase modifier subunit (GCLM) for miRNA target validation
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Specifications
Product Data | |
Vector | pMirTarget |
Species | Human |
Transfection Reporter | RFP |
Assay Reporter | Luciferase |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Symbol | GCLM |
Synonyms | GLCLR |
ACCN | NM_002061 |
Insert Size | 1987 |
Sequence Data |
>SC217196 3'UTR clone of NM_002061
The sequence shown below is from the reference sequence of NM_002061. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon CAATTGGCAGAGCTCAGAATTCAAGCGATCGC AAAAGGCTACATTTTACAAGCTAAAAGAAGGGGTTCTTAACTGACTTAGGAGCATAACTTACCTGTAATT TCCTTCAATATGAGAGAAAATTGAGATGTGTAAAAATCTAGTTACTGCCTGTAAATGGTGTCATTGAGGC AGATATTCTTTCGTCATATTTGACAGTATGTTGTCTGTCAAGTTTTAAATACTTATCTTGCCTCCATATC AATCCATTCTCATGAACCTCTGTATTGCTTTCCTTAAACTATTGTTTTCTAATTGAAATTGTCTATAAAG AAAATACTTGCAATATATTTTTCCTTTATTTTTATGACTAATATAAATCAAGAAAATTTGTTGTTAGATA TATTTTGGCCTAGGTATCAGGGTAATGTATATACATATTTTTTATTTCCAAAAAAAATTCATTAATTGCT TCTTAACTCTTATTATAACCAAGCAATTTAATTACAATTGTTAAAACTGAAATACTGGAAGAAGATATTT TTCCTGTCATTGATGAGATATATCAGAGTAACTGGAGTAGCTGGGATTTACTAGTAGTGTAAATAAAATT CACTCTTCAATACATGAATGGAAACTTAAATTTTTTTTTATGTGTCCTTGCTTATAGTTTAGCTGTAATA ATTTAACCTTGTATTCTTGTGCCATATTCTGTCTTTTTATTACTTATAAAGACAAACCAAAGTAAATCTG AAAGGAGACTAGAAGCTTTGAAATTATTGTTTGGGGGTTTTATAAAAGCAACTACTGTCACCTCCATCCA GATTCTTTTAAATTATTGATCCATCCATAGTATATATTGCTACTCATTCAAGAATCCTCAATAAGTATTG AGTATTTACCATATGTTGGGATACTGTGGGCTCTGGAGAGAGGAGGGGGCAATAGAGCTAGGAATTAAGA ATCAGTTGAGTAAAATGTGTAATATTTATTCCCCATTAATAACTGACTAGGAAGGACTAAAAGCCAGAAA GGGGATGAAAAAAAAATCCTTAATTCAGGGCCGACATTATCTACTTAAACAACTTTGAGATATGGTCTTA ATTATTTTAAAGCAGAATAATATAATTGAAAGTTTATAGCTAAAAGAGACTATATAGGTCATTTAGTATA ATTCTTCATTAGTTTACGAACCACAAAATTGCAAATAAATAAGCTATGAACTTTGATGTACACTATAAAT CTCCTTAATTCTATAAATTTGTGTCTGTAACCTGAATAGTTTGAAAACTTCTTTAAAAATCTCTTGTATT TCATCCGGGCGCAGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCCGAGGTGGGCAGATCACGAGG TCAGGAGTTTGAGACCAGCCTGACCAACATGGTAAAACCCCATCTCTACTAAAATACAAAAATTGGCTGG GCGTGGTGGCACTCGCCTGTAATCTCAGCTACTTGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGA GGCGGAGGTTACAGTGAGCCGAGATCACATCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCCATCT CAAAAAAAAAAAAAAACTCTTGTATCTCAATATTTTTAAACCACAGGCCTAAATAAAACTAATTTTGCTC AAGTTTTCTCAACCTAGGGAAAAAGAACTATGGTTCCATATTCAAAATAAATATTATAGACCCTTTTCCT AAGTAGGATTTTGTGGTTTACTGATTGGGTAATTTGATCATTAAAATTATGTGAAATCTGCCCGGGCACA CCTCATGCCTGTAATCCCAGCACTCTGGGAGGCCAAGGCAGATGATCACCTGAGGTCAGGAGTTCTAGAC CAGCCTGGCTAACATGGTGAAACCCTGTATCTGCTAAAAATACAAAAATTAGCCAGGCGTGGTGGCGGGC TCCTGTAATCCCAGCTACTTTGGAGGCGAGGCACGAGAATCGCTTGAACCTGGGAGGCGGAGTTTGCAGT GAGCCGAGATCACGCCATTGCACTCCA ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG |
Restriction Sites | SgfI-MluI |
OTI Disclaimer | Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs). |
Reference Data | |
RefSeq | NM_002061.2 |
Summary | Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015] |
Locus ID | 2730 |
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